Arginine Catabolism in the Cyanobacterium
            <i>Synechocystis</i>
            sp. Strain PCC 6803 Involves the Urea Cycle and Arginase Pathway

Quintero, Marı́a José; Muro‐Pastor, Alicia M.; Herrero, Antonia; Flores, Enrique

doi:10.1128/jb.182.4.1008-1015.2000

Cited by 73 publications

(93 citation statements)

References 49 publications

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“…In Synechocystis, an arginine catabolic pathway has been described that combines the arginase pathway and urea cycle (11). All proteins in this pathway were detected in our study.…”

Section: Alternate Pathway For Assimilation Of Nitrogen and Carbon Unmentioning

confidence: 54%

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Global Proteomics Reveal an Atypical Strategy for Carbon/Nitrogen Assimilation by a Cyanobacterium Under Diverse Environmental Perturbations

Wegener

Singh

Jacobs

et al. 2010

Molecular & Cellular Proteomics

111

133

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“…In Synechocystis, an arginine catabolic pathway has been described that combines the arginase pathway and urea cycle (11). All proteins in this pathway were detected in our study.…”

Section: Alternate Pathway For Assimilation Of Nitrogen and Carbon Unmentioning

confidence: 54%

“…There are at least five known pathways for the catabolism of arginine in prokaryotes. Among them, a pathway utilizing arginase and the urea cycle has been shown to be active in Synechocystis (11).…”

mentioning

confidence: 99%

Global Proteomics Reveal an Atypical Strategy for Carbon/Nitrogen Assimilation by a Cyanobacterium Under Diverse Environmental Perturbations

Wegener

Singh

Jacobs

et al. 2010

Molecular & Cellular Proteomics

111

133

View full text Add to dashboard Cite

“…Other examples include Quintero et al (2000), who generated mutants showing an impaired Arg synthesis as a result of a knockout of gene sll0902, coding for an Orn carbamoyltransferase (ArgF). Also, an insertion in the gene (slr0661) coding a pyrroline-5-carboxylate reductase (ProC) resulted in impaired Pro synthesis.…”

Section: Comparison Of In Silico Knockout Mutants and In Vivo Measurementioning

confidence: 99%

The Metabolic Network of Synechocystis sp. PCC 6803: Systemic Properties of Autotrophic Growth

et al. 2010

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and Manchester Interdisciplinary Biocentre, University of Manchester, M1 7DN Manchester, United Kingdom (R.S.) Unicellular cyanobacteria have attracted growing attention as potential host organisms for the production of valuable organic products and provide an ideal model to understand oxygenic photosynthesis and phototrophic metabolism. To obtain insight into the functional properties of phototrophic growth, we present a detailed reconstruction of the primary metabolic network of the autotrophic prokaryote Synechocystis sp. PCC 6803. The reconstruction is based on multiple data sources and extensive manual curation and significantly extends currently available repositories of cyanobacterial metabolism. A systematic functional analysis, utilizing the framework of flux-balance analysis, allows the prediction of essential metabolic pathways and reactions and allows the identification of inconsistencies in the current annotation. As a counterintuitive result, our computational model indicates that photorespiration is beneficial to achieve optimal growth rates. The reconstruction process highlights several obstacles currently encountered in the context of large-scale reconstructions of metabolic networks.Cyanobacteria are among the evolutionarily oldest organisms and are the only known prokaryotes capable of plant-like oxygenic photosynthesis. As primary producers in aquatic environments, they play an important role in global CO 2 assimilation and oxygen recycling. Recently, cyanobacteria have also attracted growing attention for economic purposes, including drug discovery and as prolific producers of natural products (Sielaff et al., 2006;Tan, 2007). In particular their ability to directly convert atmospheric CO 2 into biomass and organic compounds, driven by sunlight, offers considerable potential as a novel and renewable resource for bioenergy (Deng and Coleman, 1999;Atsumi et al., 2009;Mascarelli, 2009;Lindberg et al., 2010).Among the diverse cyanobacterial strains, Synechocystis sp. PCC 6803 is one of the most extensively studied model organisms for the analysis of photosynthetic processes. With a rich compendium of genomic, biochemical, and physiological data available, Synechocystis sp. PCC 6803, therefore, offers an ideal starting point to obtain insights into the systemic properties of phototrophic metabolism. The prerequisite for such a systemic description is a detailed reconstruction of the metabolic network of the organism: that is, a reconstruction of the comprehensive set of enzyme-catalyzed reactions required to support cellular growth and maintenance. Once a metabolic reconstruction is available, the vast array of methods developed by computational systems biology over the past decades allows us to dissect the functioning and interplay of possible metabolic routes and biochemical interconversions. In this respect, constraint-based modeling, most notably flux-balance analysis (FBA), has become a quasi-standard in the field. FBA is increasingly utilized to elucidate and characterize largescale network...

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“…The efficiency of CO 2 fixation and assimilation is, at least partially, under the control of a carbon concentration mechanism, which increases the CO 2 concentration around the Rubisco catalytic site (Price et al, 1998), allowing it to operate close to its V max (Badger et al, 2006). Synechocystis can take up and metabolize different nitrogen sources (nitrate, nitrite, ammonium, urea, and some amino acids; Quintero et al, 2000;Valladares et al, 2002;Muro-Pastor et al, 2005). Intriguingly, the control of the expression and activity of transporters and enzymes related to nitrogen metabolism is regulated by a complex network involving 2-oxoglutarate, NtcA (nitrogen control factor of cyanobacteria), PII (nitrogen regulatory protein PII), and PipX (PII-interacting protein X; Alfonso et al, 2001;Herrero et al, 2001;Llácer et al, 2010;Zhao et al, 2010;Espinosa et al, 2014;Lüddecke and Forchhammer, 2015).…”

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confidence: 99%

A Novel Mechanism, Linked to Cell Density, Largely Controls Cell Division in Synechocystis

et al. 2017

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ORCID IDs: 0000-0001-7618-4748 (M.I.); 0000-0001-8931-7722 (T.O.); 0000-0002-6113-9570 (R.S.).Many studies have investigated the various genetic and environmental factors regulating cyanobacterial growth. Here, we investigated the growth and metabolism of Synechocystis sp. PCC 6803 under different nitrogen sources, light intensities, and CO 2 concentrations. Cells grown on urea showed the highest growth rates. However, for all conditions tested, the daily growth rates in batch cultures decreased steadily over time, and stationary phase was obtained with similar cell densities. Unexpectedly, metabolic and physiological analyses showed that growth rates during log phase were not controlled primarily by the availability of photoassimilates. Further physiological investigations indicated that nutrient limitation, quorum sensing, light quality, and light intensity (self-shading) were not the main factors responsible for the decrease in the growth rate and the onset of the stationary phase. Moreover, cell division rates in fed-batch cultures were positively correlated with the dilution rates. Hence, not only light, CO 2 , and nutrients can affect growth but also a cell-cell interaction. Accordingly, we propose that cell-cell interaction may be a factor responsible for the gradual decrease of growth rates in batch cultures during log phase, culminating with the onset of stationary phase.

show abstract

Arginine Catabolism in the Cyanobacterium Synechocystis sp. Strain PCC 6803 Involves the Urea Cycle and Arginase Pathway

Cited by 73 publications

References 49 publications

Global Proteomics Reveal an Atypical Strategy for Carbon/Nitrogen Assimilation by a Cyanobacterium Under Diverse Environmental Perturbations

Global Proteomics Reveal an Atypical Strategy for Carbon/Nitrogen Assimilation by a Cyanobacterium Under Diverse Environmental Perturbations

The Metabolic Network of Synechocystis sp. PCC 6803: Systemic Properties of Autotrophic Growth

A Novel Mechanism, Linked to Cell Density, Largely Controls Cell Division in Synechocystis

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