ARF2 positively regulates flavonols and proanthocyanidins biosynthesis in Arabidopsis thaliana

Jiang, Wenbo; Yin, Xia; Su, Xiaojia; Pang, Yongzhen

doi:10.1007/s00425-022-03936-w

Cited by 18 publications

(4 citation statements)

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“…A study revealed that auxin may impact flavonol accumulation through the regulation of ARF protein on CHS, CHI, FLS , and F3’H [ 78 ]. In Arabidopsis thaliana, ARF2 directly regulates the expression of MYB12 and FLS genes , and indirectly regulates MYB11 and MYB111 genes increased flavonol content [ 22 ]. Additionally, it was discovered that a WDR gene (AN11) may control flavonol production with bHLH and R2R3-MYB proteins in Entada phaseoloides [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

“…Flavonoids are initially synthesized catalyzed by phenylalanine ammonia-lyase (PAL), followed by cinnamate 4-hydroxylase (C4H), 4-coumarate: CoA ligase (4CL), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), flavonol synthase (FLS), and UDP-glucosyltransferase (UGT) [20]. Transcription factors (TFs) such as bZIP, WRKY, MADSbox, ARF, NAC, and especially bHLH, MYB, and WD40 controlled flavonoid biosynthesis through regulating the biosynthetic genes involved in flavonoid biosynthesis pathway [21][22][23]. Among these, bHLH TFs can control anthocyanin, PA, and flavonol biosynthesis within the flavonoid pathway [24].…”

Section: Introductionmentioning

confidence: 99%

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Integrated transcriptomic and WGCNA analyses reveal candidate genes regulating mainly flavonoid biosynthesis in Litsea coreana var. sinensis

Xie,

Guo,

et al. 2024

BMC Plant Biol

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Litsea coreana Levl. var. sinensis (Allen) Yang et P. H. Huang is a popular ethnic herb and beverage plant known for its high flavonoid content, which has been linked to a variety of pharmacological benefits and crucial health-promoting impacts in humans. The progress in understanding the molecular mechanisms of flavonoid accumulation in this plant has been hindered due to the deficiency of genomic and transcriptomic resources. We utilized a combination of Illumina and Oxford Nanopore Technology (ONT) sequencing to generate a de novo hybrid transcriptome assembly. In total, 126,977 unigenes were characterized, out of which 107,977 were successfully annotated in seven public databases. Within the annotated unigenes, 3,781 were categorized into 58 transcription factor families. Furthermore, we investigated the presence of four valuable flavonoids—quercetin-3-O-β-D-galactoside, quercetin-3-O-β-D-glucoside, kaempferol-3-O-β-D-galactoside, and kaempferol-3-O-β-D-glucoside in 98 samples, using high-performance liquid chromatography. A weighted gene co-expression network analysis identified two co-expression modules, MEpink and MEturquoise, that showed strong positive correlation with flavonoid content. Within these modules, four transcription factor genes (R2R3-MYB, NAC, WD40, and ARF) and four key enzyme-encoding genes (CHI, F3H, PAL, and C4H) emerged as potential hub genes. Among them, the R2R3-MYB (LcsMYB123) as a homologous gene to AtMYB123/TT2, was speculated to play a significant role in flavonol biosynthesis based on phylogenetic analysis. Our findings provided a theoretical foundation for further research into the molecular mechanisms of flavonoid biosynthesis. Additionally, The hybrid transcriptome sequences will serve as a valuable molecular resource for the transcriptional annotation of L. coreana var. sinensis, which will contribute to the improvement of high-flavonoid materials.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Integrated transcriptomic and WGCNA analyses reveal candidate genes regulating mainly flavonoid biosynthesis in Litsea coreana var. sinensis

Xie,

Guo,

et al. 2024

BMC Plant Biol

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“…Surprisingly, the gene correlation network analysis showed that genes in the auxin-related pathway and the calmodulin-binding protein pathway (Zosma01g26740, Zosma05g26860, Zosma06g11260, and Zosma06g17940) and flavonoid biosynthesis (Zosma06g08180 and Zosma06g25630) had a strong positive correlation ( Supplementary Figure 5C ). Studies in Arabidopsis have shown that auxin response factor 2 plays a positive regulatory role in the synthesis and accumulation of flavonoids ( Jiang et al., 2022 ). This revealed that similar to terrestrial plants, NO also regulates antioxidant substances such as flavonoids in response to high-salt environments in eelgrass leaves.…”

Section: Discussionmentioning

confidence: 99%

NO enhances the adaptability to high-salt environments by regulating osmotic balance, antioxidant defense, and ion homeostasis in eelgrass based on transcriptome and metabolome analysis

Wang,

et al. 2024

Front. Plant Sci.

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IntroductionEelgrass is a typical marine angiosperm that exhibits strong adaptability to high-salt environments. Previous studies have shown that various growth and physiological indicators were significantly affected after the nitrate reductase (NR) pathway for nitric oxide (NO) synthesis in eelgrass was blocked.MethodsTo analyze the molecular mechanism of NO on the adaptability to high-salt environment in eelgrass, we treated eelgrass with artificial seawater (control group) and artificial seawater with 1 mM/L Na2WO4 (experimental group). Based on transcriptomics and metabolomics, we explored the molecular mechanism of NO affecting the salt tolerance of eelgrass.ResultsWe obtained 326, 368, and 859 differentially expressed genes (DEGs) by transcriptome sequencing in eelgrass roots, stems, and leaves, respectively. Meanwhile, we obtained 63, 52, and 36 differentially accumulated metabolites (DAMs) by metabolomics in roots, stems, and leaves, respectively. Finally, through the combined analysis of transcriptome and metabolome, we found that the NO regulatory mechanism of roots and leaves of eelgrass is similar to that of terrestrial plants, while the regulatory mechanism of stems has similar and unique features.DiscussionNO in eelgrass roots regulates osmotic balance and antioxidant defense by affecting genes in transmembrane transport and jasmonic acid-related pathways to improve the adaptability of eelgrass to high-salt environments. NO in eelgrass leaves regulates the downstream antioxidant defense system by affecting the signal transduction of plant hormones. NO in the stems of eelgrass regulates ion homeostasis by affecting genes related to ion homeostasis to enhance the adaptability of eelgrass to high-salt environments. Differently, after the NO synthesis was inhibited, the glyoxylate and dicarboxylate metabolism, as well as the tricarboxylic acid (TCA) cycle, was regulated by glucose metabolism as a complementary effect to cope with the high-salt environment in the stems of eelgrass. These are studies on the regulatory mechanism of NO in eelgrass, providing a theoretical basis for the study of the salt tolerance mechanism of marine plants and the improvement of terrestrial crop traits. The key genes discovered in this study can be applied to increase salt tolerance in terrestrial crops through cloning and molecular breeding methods in the future.

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“…3 ). Recently, AtARF2 was identified as a positive regulator of flavonol biosynthesis through directly activating transcription of the AtMYB12 and AtFLS genes [ 131 ]. Another study indicated that auxin-induced flavonol accumulation also depended on the ARF pathway [ 5 ] ( Fig.…”

Section: Cascade Transcriptional Regulation Of Flavonol Biosynthesismentioning

confidence: 99%

Transcriptional regulation of flavonol biosynthesis in plants

Cao,

Mei,

Zhang

et al. 2024

Horticulture Research

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Summary Flavonols are a class of flavonoids that play a crucial role in regulating plant growth and promoting stress resistance. They are also important dietary components in horticultural crops due to their benefits for human health. In past decades, research on the transcriptional regulation of flavonol biosynthesis in plants has increased rapidly. This review summarized recent progress in the flavonol-specific transcriptional regulation in plants, encompassing characterization of different categories of transcription factors (TFs) and microRNAs as well as elucidation of different transcriptional mechanisms including direct and cascade transcriptional regulation. The direct transcriptional regulation consists of TFs, such as MYB, AP2/ERF, WRKY, which can directly target the key flavonol synthase gene or other early genes in the flavonoid biosynthesis. In addition, different regulation modules in the cascade transcriptional regulation involve microRNAs targeting TFs, regulation between activators, interaction between activators and repressors, and degradation of activators or repressors induced by UV-B light or plant hormones. Such sophisticated regulation of the flavonol biosynthetic pathway in response to UV-B radiation or hormones may allow plants to fine-tune flavonol homeostasis, thereby balances plant growth and stress responses in a timely manner. Based on the orchestrated regulation, molecular design strategies will be applied to breed horticultural crops with excellent health-promoting effects and high resistance.

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ARF2 positively regulates flavonols and proanthocyanidins biosynthesis in Arabidopsis thaliana

Cited by 18 publications

References 73 publications

Integrated transcriptomic and WGCNA analyses reveal candidate genes regulating mainly flavonoid biosynthesis in Litsea coreana var. sinensis

Integrated transcriptomic and WGCNA analyses reveal candidate genes regulating mainly flavonoid biosynthesis in Litsea coreana var. sinensis

NO enhances the adaptability to high-salt environments by regulating osmotic balance, antioxidant defense, and ion homeostasis in eelgrass based on transcriptome and metabolome analysis

Transcriptional regulation of flavonol biosynthesis in plants

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