Arenibacter aquaticus sp. nov., a marine bacterium isolated from surface sea water in the South China Sea

Abstract

A Gram-stain-negative and facultatively anaerobic bacterial strain, designated GUOT, was isolated from surface water collected from the South China Sea. Cells were non-flagellate, yellow, non-spore-forming and rod-shaped. The 16S rRNA gene sequence comparisons with species in the genus Arenibacter showed that strain GUOT shares the highest similarity of 97.5 % with Arenibacter echinorum and Arenibacter palladensis . Average nucleotide identity and digital DNA–DNA hybridization values between strain GUOT and its related type strains were 77.1–78.4% and 20.8–26.2 % respectively. Growth of strain GUOT occurred at 15–50°C (optimum, 20–25°C), pH 5–7.5 (pH 6) and in media containing 0–7 % NaCl (optimum, 0–1 %). Cells contained methanol-soluble yellow-coloured pigments but flexirubin-type pigments were absent. The major fatty acids (>5 %) were iso-C17 : 0 3-OH, iso-C15 : 0, anteiso-C15 : 0, C16 : 0, summed feature 3, iso-C15 : 1 G and iso-C15 : 0 3-OH. The dominant polar lipids comprised phosphatidylethanolamine and some unidentified polar lipids. The main respiratory quinone was menaquinone-6. The DNA G+C content of strain GUOT was 40.1 %. Based on the presented data, we consider strain GUOT to represent a novel species of the genus Arenibacter , for which the name Arenibacter aquaticus sp. nov. is proposed. The type strain is GUOT (=KCTC 62629T=MCCC 1K03559T)

Pseudopontixanthobacter vadosimaris gen. nov., sp. nov., isolated from shallow sea near Kueishan Island

“…The genome was sequenced by an Illumina HiSeq 4000 system (Shanghai Hanyu Bio-Tech). Raw data were filtered, evaluated and then de novo assembled as described previously [25]. Average amino acid identity (AAI) values between strain JL3514 T and related strains from the family Erythrobacteraceae were calculated with the Kostas lab AAI Calculator (http://enve-omics.ce.gatech.edu/aai/) [26].…”

Arenibacter amylolyticus sp. nov., an amylase-producing bacterium of the family Flavobacteriaceae isolated from marine water in India

“…The type species is Arenibacter latericius [1]. The genus Arenibacter presently comprises ten validly described species (https://lpsn.dsmz.de/genus/arenibacter) and species have been isolated from different substrates and geographic locations such as green alga Ulva fenestrata [2, 3], sea urchin Strongylocentrotus intermedius [4], surface sea water [5], marine tidal flat sediment [6, 7], marine bottom sediment [8, 9] and marine sandy sediment [1, 10]. In the present study, we focused on the characterization and classification of strain AK53 T [11], which was isolated from a 5 m depth marine water sample collected from Visakhapatnam, India, and based on our polyphasic taxonomic results assign the novel strain to the genus Arenibacter .…”

Arenibacter arenosicollis sp. nov., isolated from a sand dune

“…Individual polar lipids were identified by spraying reagents as described Park et al [24]. The predominant isoprenoid quinone detected in strain BSSL-BM3 T was menaquinone-6 (MK-6), which is consistent those of members of the family Flavobacteriaceae [1] as well as Arenibacter species [2,[4][5][6][7]. The major fatty acids (>10 % of the total fatty acids in all growth phases) found in strain BSSL-BM3 T were iso-C 17 : 0 3-OH, summed feature 3 (C 16 : 1 ω7c and/or C 16 : 1 ω6c) and iso-C 15 : 1 G ( Table 2).…”

“…At the time of writing, the genus Arenibacter comprises 10 species with validly published names (https://lpsn.dsmz.de/genus/arenibacter [3]). Members of the genus Arenibacter are characterized to be aerobic, Gram-stain-negative, oxidase-positive and by having DNA G+C contents of 36.8–40.1 mol%, menaquinone-6 as predominant isoprenoid and phosphatidylethanolamine as only identified major phospholipid [2, 4–7]. Members assigned into the genus Arenibacter have been isolated from marine organisms and marine environments including marine sediments, seawater and tidal flat [2, 4–12].…”

“…Members of the genus Arenibacter are characterized to be aerobic, Gram-stain-negative, oxidase-positive and by having DNA G+C contents of 36.8-40.1 mol%, menaquinone-6 as predominant isoprenoid and phosphatidylethanolamine as only identified major phospholipid [2,[4][5][6][7]. Members assigned into the genus Arenibacter have been isolated from marine organisms and marine environments including marine sediments, seawater and tidal flat [2,[4][5][6][7][8][9][10][11][12]. Recently, in the course of screening novel bacteria from a sand dune near the Yellow Sea, Republic of Korea, many novel bacterial strains have been isolated and characterized taxonomically.…”