Arenaviruses

Salvato, María S.; Rodas, Juan David

doi:10.1002/9780470688618.taw0255

Cited by 1 publication

(1 citation statement)

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“…Since the RING domains of arenaviral Z proteins are highly conserved [36], it is likely that all arenaviruses will have similar effects on PML NB. In addition, PML is prominently expressed in reticuloendothelial cells [37], the most frequent targets of arenavirus infection [38]. The confocal images presented here clearly showed that PML is within discrete NB that are subject to dramatic reorganization during LCMV infection and that NSC20625 successfully inhibited these viral events.…”

Section: Discussionmentioning

confidence: 69%

An antiviral disulfide compound blocks interaction between arenavirus Z protein and cellular promyelocytic leukemia protein

Topisirović

Djavani

Borden

et al. 2010

Biochemical and Biophysical Research Communications

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The promyelocytic leukemia protein (PML) forms nuclear bodies (NB) that can be redistributed by virus infection. In particular, lymphocytic choriomeningitis virus (LCMV) influences disruption of PML NB through the interaction of PML with the arenaviral Z protein. In a previous report, we have shown that the disulfide compound NSC20625 has antiviral and virucidal properties against arenaviruses, inducing unfolding and oligomerization of Z without affecting cellular RINGcontaining proteins such as the PML. Here, we further studied the effect of the zinc-finger-reactive disulfide NSC20625 on PML-Z interaction. In HepG2 cells infected with LCMV or transientlytransfected with Z-protein constructs, treatment with NSC20625 restored PML distribution from a diffuse cytoplasmic pattern to punctate, discrete NB which appeared identical to NB found in control, uninfected cells. Similar results were obtained in cells transfected with a construct expressing a Z mutant in zinc-binding site 2 of the RING domain, confirming that this Z-PML interaction requires the integrity of only one zinc-binding site. Altogether, these results show that the compound NSC20625 suppressed Z-mediated PML NB disruption and may be used as a tool for designing novel antiviral strategies against arenavirus infection.

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Section: Discussionmentioning

confidence: 69%