Area-Wide Mosquito Management in Lee County, Florida, USA

Foley, E. W.; Morreale, Rachel; Hoel, David F.; Lloyd, Aaron

doi:10.1201/9781003169239-18

Cited by 4 publications

(5 citation statements)

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“…technique is being currently analysed in different environments with promising results (Bouyer et al, 2020;Foley et al, 2021); however, its regulatory status still needs to be defined. B. germanica is also the responsible intermediate host of Gongylonema pulchrum, a nematode of the digestive system of livestock (Brenner & Kramer, 2019).…”

Section: The Possible Role Of Sterile Insect Technique and Incompatib...mentioning

confidence: 99%

“…Insecticide resistance among mosquito species vectoring human and animal diseases is seriously threatening the control of such vectors (Airs & Bartholomay, 2017). The possible role of sterile insect technique and incompatible insect technique is being currently analysed in different environments with promising results (Bouyer et al, 2020; Foley et al, 2021); however, its regulatory status still needs to be defined. The most practical way to apply dsRNA‐based bioinsecticides could be directed to those areas where water stagnation can occur, for example, drinking troughs, washing tanks, irrigation pumps and wells, slurry collection tanks, and so forth.…”

Section: Developing Delivery Systems For Dsrna In Animal Farmsmentioning

confidence: 99%

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The use of RNA interference for the management of arthropod pests in livestock farms

Bonina,

Arpaia

2023

Medical Vet Entomology

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Pest management in farm animals is an important action to contain economic damage to livestock production and prevent transmission of severe diseases to the stock. The use of chemical insecticides is still the most common approach followed by farmers; however, avoiding possible toxic effects on animals is a fundamental task for pest control measures compatible with animal well‐being. Moreover, legal constraints and insurgence of resistance by target species to the available insecticidal compounds are increasingly complicating farmers' operations. Alternatives to chemical pesticides have been explored with some promising results in the area of biological control or the use of natural products as sprays. The application of RNA interference techniques has enabled the production of new means of pest control in agriculture, and it is opening a promising avenue for controlling arthropod pests of livestock. Transcript depletion of specific target genes of the recipient organisms is based on the action of double‐strand RNAs (dsRNA) capable of impairing the production of fundamental proteins. Their mode of action, based on the specific recognition of short genomic sequences, is expected to be highly selective towards non‐target organisms potentially exposed; in addition, there are physical and chemical barriers to dsRNA uptake by mammalian cells that render these products practically innocuous for higher animals. Summarising existing literature on gene silencing for main taxa of arthropod pests of livestock (Acarina, Diptera, Blattoidea), this review explores the perspectives of practical applications of dsRNA‐based pesticides against the main pests of farm animals. Knowledge gaps are summarised to stimulate additional research in this area.

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Section: The Possible Role Of Sterile Insect Technique and Incompatib...mentioning

confidence: 99%

Section: Developing Delivery Systems For Dsrna In Animal Farmsmentioning

confidence: 99%

The use of RNA interference for the management of arthropod pests in livestock farms

Bonina,

Arpaia

2023

Medical Vet Entomology

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“…aegypti since 2017, with the goal of achieving an in-house sterile insect production facility capable of producing enough sterile male Ae. aegypti for an area-wide control program (Foley et al 2021, Carvalho et al 2022). In a multi-year pilot program, LCMCD has successfully produced 400,000 sterile males per week for continuous release, but developmental synchronization of larvae into pupae for irradiation remains an issue that periodically delays irradiation and release, resulting in scheduling conflicts and increased costs of labor.…”

Section: Introductionmentioning

confidence: 99%

Developing methods for chilling, compacting, and sterilizing adult Aedes aegypti (Diptera: Culicidae) and comparing mating competitiveness between males sterilized as adults versus pupae for sterile male release

Tussey

Morreale

Carvalho

et al. 2023

Journal of Medical Entomology

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The yellow fever mosquito, Aedes aegypti L., can transmit several pathogens responsible for human diseases. With insecticide resistance development becoming a concern, alternative control strategies are needed for Ae. aegypti. Sterile insect technique (SIT) is an increasingly popular option being explored. However, logistical issues in mass production and sterilization make it difficult to maintain a SIT program. Male mosquitoes are typically irradiated as pupae because this is the earliest developmental point at which females can be separated from males, but asynchrony in pupation and high variability in pupal responses to irradiation based on pupal age make it difficult to sterilize mass quantities of pupae on a regular schedule in a rearing facility. Young adult mosquitoes have wider windows for irradiation sterilization than pupae, which can allow facilities to have fixed schedules for irradiation. We produced a workflow for adult Ae. aegypti irradiation in a mosquito control district with an operational SIT program that currently irradiates pupae. The impacts of chilling, compaction, and radiation dose on survival were all assessed before combining them into a complete adult irradiation protocol. Males chilled up to 16 h prior to compaction and compacted to 100 males/cm3 during radiation resulted in low mortality. Males irradiated as adults had increased longevity and similar sterility compared to males irradiated as pupae. Additionally, males sterilized as adults were more sexually competitive than males sterilized as pupae. Thus, we have shown that irradiating adult males can be a viable option to increase the efficiency of this operational mosquito SIT program.

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“…Established in 1958 as an independent special taxing district, LCMCD operates a robust program aimed at mosquito suppression. Using an area-wide integrated pest management approach, efforts include chemical suppression of both the larval and adult stages, disease surveillance, mosquito population surveillance, and an insecticide-resistance monitoring program [ 25 ]. In 2017, LCMCD initiated a SIT pilot program in Captiva Island, Florida, USA, a ~ 230-ha barrier island, with the aim of establishing an operational SIT program for Ae.…”

Section: Introductionmentioning

confidence: 99%

A sterile insect technique pilot trial on Captiva Island: defining mosquito population parameters for sterile male releases using mark–release–recapture

et al. 2022

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Background The sterile insect technique (SIT), which involves area-wide inundative releases of sterile insects to suppress the reproduction of a target species, has proven to be an effective pest control method. The technique demands the continuous release of sterilized insects in quantities that ensure a high sterile male:wild male ratio for the suppression of the wild population over succeeding generations. Methods For these releases, it is important to determine several ecological and biological population parameters, including the longevity of the released males in the field, the dispersal of the released males and the wild pest population size. The Lee County Mosquito Control District initiated a study in a 47-ha portion of Captiva Island (Florida, USA), an island with a total area of 230 ha, to define biological SIT parameters for Aedes aegypti (L.), an invasive disease-vectoring mosquito known to be difficult to control due to a combination of daytime biting activity, use of cryptic breeding habitats that are difficult to target with conventional night-time ultra-low volume methods, and emerging resistance to commonly used insecticides. Another goal was to assess patterns of dispersal and survival for laboratory-reared sterile Ae. aegypti males released over time in the pilot site. These parameters will be used to evaluate the efficacy of a SIT suppression program for Ae. aegypti on Captiva Island. Results Over the course of seven mark-release-recapture studies using single- and multiple-point releases, 190,504 sterile marked males were released, for which the recapture rate was 1.5% over a mean period of 12 days. The mean distance traveled by sterile males of the local strain of Ae. aegypti that has colonized Captiva Island was 201.7 m from the release point, with an observed maximum traveled distance of 404.5 m. The released sterile mosquitoes had a probability of daily survival of 0.67 and an average life expectancy of ~ 2.46 days. Conclusions These data together with the population size estimate and sterile:wild ratio provide a solid basis for planning the SIT operational phase which is aimed at mosquito population suppression. Graphical abstract

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Area-Wide Mosquito Management in Lee County, Florida, USA

Cited by 4 publications

References 1 publication

The use of RNA interference for the management of arthropod pests in livestock farms

The use of RNA interference for the management of arthropod pests in livestock farms

Developing methods for chilling, compacting, and sterilizing adult Aedes aegypti (Diptera: Culicidae) and comparing mating competitiveness between males sterilized as adults versus pupae for sterile male release

A sterile insect technique pilot trial on Captiva Island: defining mosquito population parameters for sterile male releases using mark–release–recapture

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