Are we ready to inject? Individualized LC-CUSUM training in ICSI

Durbán, Mercé; García, Désirée; Obradors, Albert; Vernaeve, V.; Vassena, Rita

doi:10.1007/s10815-016-0686-4

Cited by 8 publications

(6 citation statements)

References 14 publications

(18 reference statements)

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“…Intracytoplasmic sperm injection is routinely performed on multiple oocytes from a single patient [ 13 , 14 ]. As a result, multiple oocytes are injected sequentially within the same microliter volume of medium.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication on the microscale: a two-photon polymerized device for oocyte microinjection

Yagoub

Thompson

Orth

et al. 2022

J Assist Reprod Genet

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Purpose Intracytoplasmic sperm injection (ICSI) addresses male sub-fertility by injecting a spermatozoon into the oocyte. This challenging procedure requires the use of dual micromanipulators, with success influenced by inter-operator expertise. We hypothesized that minimizing oocyte handling during ICSI will simplify the procedure. To address this, we designed and fabricated a micrometer scale device that houses the oocyte and requires only one micromanipulator for microinjection. Methods The device consisted of 2 components, each of sub-cubic millimeter volume: a Pod and a Garage. These were fabricated using 2-photon polymerization. Toxicity was evaluated by culturing single-mouse presumptive zygotes (PZs) to the blastocyst stage within a Pod, with several Pods (and embryos) docked in a Garage. The development was compared to standard culture. The level of DNA damage/repair in resultant blastocysts was quantified (γH2A.X immunohistochemistry). To demonstrate the capability to carry out ICSI within the device, PZs were microinjected with 4-μm fluorescent microspheres and cultured to the blastocyst stage. Finally, the device was assessed for oocyte traceability and high-throughput microinjection capabilities and compared to standard microinjection practice using key parameters (pipette setup, holding then injecting oocytes). Results Compared to standard culture, embryo culture within Pods and a Garage showed no differences in development to the blastocyst stage or levels of DNA damage in resultant blastocysts. Furthermore, microinjection within our device removes the need for a holding pipette, improves traceability, and facilitates high-throughput microinjection. Conclusion This novel device could improve embryo production following ICSI by simplifying the procedure and thus decreasing inter-operator variability.

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Section: Introductionmentioning

confidence: 99%

“…Intracytoplasmic sperm injection can be a technically challenging procedure with its success being influenced by human variability [ 12 , 14 , 15 ]. This procedural variation can induce mechanical stress on the oocyte, negatively impacting fertilization [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication on the microscale: a two-photon polymerized device for oocyte microinjection

Yagoub

Thompson

Orth

et al. 2022

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…A positive linear association has been previously demonstrated between the extent of an embryologist's skills with ICSI and enhanced oocyte survival and successful fertilization [18,19]. This finding might be attributed in part to the speed acquired with training and experience in performing necessary procedures.…”

Section: Technical Expertisementioning

confidence: 83%

The value of the modern embryologist to a successful IVF system: revisiting an age-old question

Choucair

Younis

Hourani

2021

Middle East Fertil Soc J

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Background The embryology laboratory remains one of the primary factors influencing the overall performance of the IVF clinic. Clinical embryology, however, has undergone remarkable advancements over the last decade. These developments in both practice and education have been driven together by science and technology. Main body The changes in clinical embryology practice have created a significant challenge for both the profession and the embryologist. New competencies for embryologists have subsequently emerged to provide and manage high-quality services for their patients and clinics. Embryologists’ efficacy behind the scenes reflects positively on the success of the fertility clinic. This framework will serve as the flagship document to shed light on the often-overlooked value of the embryologist as an intellectual and intangible asset for the clinic. Conclusion To fully leverage a modern embryologist’s skill set, significant investment is required from the IVF clinics’ leadership, which is associated with a substantial return on the IVF clinic’s success. From their side, embryologists should be up to date on further developments and innovations. On the other hand, mentors need to mend the curriculum to allow for better training of high-caliber embryologists.

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“…Similarly, a previous study highlighted the role of the ICSI operator as the most important predictor for fertilization rate [9]. The effect of ICSI training especially, but not exclusively, may impact all of the embryologist's skills linearly over time from oocyte survival to successful fertilization [10,11]. This finding might be attributed in part to the speed performance of the laboratory procedures.…”

Section: Technical Expertisementioning

confidence: 95%

The value of the modern embryologist to a successful IVF system: Revisiting an age-old question

Bitar¹,

Younis²,

Hourani³

2021

Preprint

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BackgroundThe embryology laboratory remains the major factor influencing the overall performance of the IVF clinic. Clinical embryology, however, has undergone a significant change and development over the last decade. The changes and development have been science and technology-driven, in both practice and education.Main bodyThe changes in clinical embryology practice have created a significant challenge for both the profession and the embryologist. Often under-valued in the eyes of the clinic’s leadership, IVF clinic leaders must understand that embryologists have a significant impact on clinical outcomes and patient retention. They also enable many services that are significant revenue generators and may add prestige to the clinic. This framework will serve as the flagship document to extract value from the embryologist role as an intellectual intangible asset for the clinic.ConclusionTo fully leverage a modern embryologist's skill set, significant investment is required from the IVF clinics’ leadership that is, however, associated with a substantial return on the investment. From their side, embryologists should be prepared for further developments and innovations and adapt themselves to survive practicing clinical embryology. The profession needs also to prepare itself for the future to properly train high-caliber embryologists.

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Are we ready to inject? Individualized LC-CUSUM training in ICSI

Cited by 8 publications

References 14 publications

Fabrication on the microscale: a two-photon polymerized device for oocyte microinjection

Fabrication on the microscale: a two-photon polymerized device for oocyte microinjection

The value of the modern embryologist to a successful IVF system: revisiting an age-old question

The value of the modern embryologist to a successful IVF system: Revisiting an age-old question

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