Are they in or out? The Elusive Interaction between Qtracker
            <sup>®</sup>
            800 Vascular Labels and Brain Endothelial Cells

Radu, Beatrice Mihaela; Mihai, Radu; Tognoli, Cristina; Benati, Donatella; Merigo, Flavia; Assfalg, Michael; Solani, Erika; Stranieri, Chiara; Ceccon, Alberto; Pasini, Anna Maria Fratta; Cominacini, Luciano; Bramantı, Placido; Osculati, Francesco; Bertini, Giuseppe; Fabene, Paolo F.

doi:10.2217/nnm.15.120

Cited by 4 publications

(2 citation statements)

References 56 publications

(48 reference statements)

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“…Balb/c mouse primary brain endothelial microvascular cells BMVECs (#PB-BALB-5023, PELOBiotech, Germany; passages 3–6) and cells from the Balb/c mouse cell line bEnd.3 (#ATCC® CRL-2299™, ATCC, USA; passages 20–30) were cultivated as previously described 62 . Both endothelial cell types were tested for mycoplasma contamination twice per year using the Hoechst (#B2883, Sigma) DNA staining protocol.…”

Section: Methodsmentioning

confidence: 99%

“…Fura-2 AM-based Ca 2+ imaging experiments were performed on brain microvascular endothelial cells as previously described 62 . Measurements were performed using a cooled CCD camera (Clara, Andor, Northern Ireland) and a Polychrome V monochromator (Till Photonics, Germany) coupled to a SliceScope (Scientifica, UK) upright fluorescence microscope with a 40x-water immersion objective (Olympus, Japan).…”

Section: Methodsmentioning

confidence: 99%

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All muscarinic acetylcholine receptors (M1-M5) are expressed in murine brain microvascular endothelium

Radu

Osculati

Suku

et al. 2017

Sci Rep

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Clinical and experimental studies indicate that muscarinic acetylcholine receptors are potential pharmacological targets for the treatment of neurological diseases. Although these receptors have been described in human, bovine and rat cerebral microvascular tissue, a subtype functional characterization in mouse brain endothelium is lacking. Here, we show that all muscarinic acetylcholine receptors (M1-M5) are expressed in mouse brain microvascular endothelial cells. The mRNA expression of M2, M3, and M5 correlates with their respective protein abundance, but a mismatch exists for M1 and M4 mRNA versus protein levels. Acetylcholine activates calcium transients in brain endothelium via muscarinic, but not nicotinic, receptors. Moreover, although M1 and M3 are the most abundant receptors, only a small fraction of M1 is present in the plasma membrane and functions in ACh-induced Ca2+ signaling. Bioinformatic analyses performed on eukaryotic muscarinic receptors demonstrate a high degree of conservation of the orthosteric binding site and a great variability of the allosteric site. In line with previous studies, this result indicates muscarinic acetylcholine receptors as potential pharmacological targets in future translational studies. We argue that research on drug development should especially focus on the allosteric binding sites of the M1 and M3 receptors.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

All muscarinic acetylcholine receptors (M1-M5) are expressed in murine brain microvascular endothelium

Radu

Osculati

Suku

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

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Ca2+ homeostasis in brain microvascular endothelial cells

Stoica

Rusu

Staicu

et al. 2021

International Review of Cell and Molecular Biology

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The relationship between the secondary vascular system and the lymphatic vascular system in fish

Panara,

Varaliová,

Wilting

et al. 2024

Biological Reviews

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New technologies have resulted in a better understanding of blood and lymphatic vascular heterogeneity at the cellular and molecular levels. However, we still need to learn more about the heterogeneity of the cardiovascular and lymphatic systems among different species at the anatomical and functional levels. Even the deceptively simple question of the functions of fish lymphatic vessels has yet to be conclusively answered. The most common interpretation assumes a similar dual setup of the vasculature in zebrafish and mammals: a cardiovascular circulatory system, and a lymphatic vascular system (LVS), in which the unidirectional flow is derived from surplus interstitial fluid and returned into the cardiovascular system. A competing interpretation questions the identity of the lymphatic vessels in fish as at least some of them receive their flow from arteries via specialised anastomoses, neither requiring an interstitial source for the lymphatic flow nor stipulating unidirectionality. In this alternative view, the ‘fish lymphatics’ are a specialised subcompartment of the cardiovascular system, called the secondary vascular system (SVS). Many of the contradictions found in the literature appear to stem from the fact that the SVS develops in part or completely from an embryonic LVS by transdifferentiation. Future research needs to establish the extent of embryonic transdifferentiation of lymphatics into SVS blood vessels. Similarly, more insight is needed into the molecular regulation of vascular development in fish. Most fish possess more than the five vascular endothelial growth factor (VEGF) genes and three VEGF receptor genes that we know from mice or humans, and the relative tolerance of fish to whole‐genome and gene duplications could underlie the evolutionary diversification of the vasculature. This review discusses the key elements of the fish lymphatics versus the SVS and attempts to draw a picture coherent with the existing data, including phylogenetic knowledge.

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Are they in or out? The Elusive Interaction between Qtracker ^® 800 Vascular Labels and Brain Endothelial Cells

Abstract: Qtracker(®)800 nanoparticles elicit endothelial functional responses, which prompts biomedical safety evaluations and may bias the interpretation of experimental studies involving vascular imaging.

Cited by 4 publications

References 56 publications

All muscarinic acetylcholine receptors (M1-M5) are expressed in murine brain microvascular endothelium

All muscarinic acetylcholine receptors (M1-M5) are expressed in murine brain microvascular endothelium

Ca2+ homeostasis in brain microvascular endothelial cells

The relationship between the secondary vascular system and the lymphatic vascular system in fish

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Are they in or out? The Elusive Interaction between Qtracker ® 800 Vascular Labels and Brain Endothelial Cells

Abstract: Qtracker(®)800 nanoparticles elicit endothelial functional responses, which prompts biomedical safety evaluations and may bias the interpretation of experimental studies involving vascular imaging.

Cited by 4 publications

References 56 publications

All muscarinic acetylcholine receptors (M1-M5) are expressed in murine brain microvascular endothelium

All muscarinic acetylcholine receptors (M1-M5) are expressed in murine brain microvascular endothelium

Ca2+ homeostasis in brain microvascular endothelial cells

The relationship between the secondary vascular system and the lymphatic vascular system in fish

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Product

Resources

About

Are they in or out? The Elusive Interaction between Qtracker ^® 800 Vascular Labels and Brain Endothelial Cells