Are the mitochondrial cox1 and cob genes suitable markers for species of Dinophysis Ehrenberg?

Raho, Nicolás; Rodrı́guez, Francisco; Reguera, B.; Marı́n, Irma

doi:10.1016/j.hal.2013.05.012

Cited by 20 publications

(14 citation statements)

References 46 publications

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“…, as in Raho et al. ), which is similar to the location of the pseudogene insert recovered by Raho et al. () from D. saccula (GenBank Acc.…”

Section: Resultssupporting

confidence: 84%

“…Interestingly, Raho et al. () previously reported two pseudogene inserts in the cox1 gene of D. saccula , both of which were located similarly to the insert we observed; however, unlike this study, we were unable to recover the true cox1 gene along with the pseudogene.…”

Section: Discussioncontrasting

confidence: 66%

“…However, more recent work by Raho et al. () found that neither mt cox1 nor cytochrome b (cob) genes provided sufficient resolution to identify six Dinophysis species (field isolates and cultures of Dinophysis tripos Gourett, Dinophysis caudata Saville‐Kent and D. saccula ).…”

mentioning

confidence: 99%

“…Molecular methods have been developed to identify many dinoflagellate species and clades, but the taxonomic assignment of Dinophysis species using molecular characters is still unresolved due to extremely low interspecific variability within their nuclear ribosomal genes and intergenic regions, the typical targets of such studies (Raho et al 2013). Phylogenetic analysis of large subunit ribosomal DNA (LSU rDNA) sequences identified two major clades of photosynthetic Dinophysis, but could not discriminate among D. acuminata, Dinophysis dens Pavillard, Dinophysis saccula, and Dinophysis acuta (Edvardsen et al 2003).…”

mentioning

confidence: 99%

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Characterization and comparison of toxin‐producing isolates of Dinophysis acuminata from New England and Canada

Tong

Smith

Richlen

et al. 2014

Journal of Phycology

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Following the identification of the first toxic isolate of Dinophysis acuminata from the northwestern Atlantic, we conducted detailed investigations into the morphology, phylogeny, physiology, and toxigenicity of three isolates from three sites within the northeastern U.S./Canada region: Eel Pond and Martha’s Vineyard, Massachusetts, and the Bay of Fundy. Another isolate, collected from the Gulf of Mexico, was grown under the same light, temperature, and prey conditions for comparison. Despite observed phenotypic heterogeneity, morphometrics and molecular evidence classified the three northwestern Atlantic isolates as Dinophysis acuminata Claparède & Lachmann, whereas the isolate from the Gulf of Mexico was morphologically identified as D. cf. ovum. Physiological and toxin analyses supported these classifications, with the three northwestern Atlantic isolates being more similar to each other with respect to growth rate, toxin profile, and diarrhetic shellfish poisoning (DSP) toxin content (okadaic acid + dinophysistoxin 1/cell) than they were to the isolate from the Gulf of Mexico, which had toxin profiles similar to those published for D. cf. ovum F. Schütt. The DSP toxin content, 0.01 – 1.8 pg okadaic acid (OA) + dinophysistoxin (DTX1) · cell−1, of the three northwestern Atlantic isolates was low relative to other D. acuminata strains from elsewhere in the world, consistent with the relative scarcity of shellfish harvesting closures due to DSP toxins in the northeastern U.S. and Canada. If this pattern is repeated with analyses of more geographically and temporally dispersed isolates from the region, it would appear that the risk of significant DSP toxin outbreaks in the northwestern Atlantic is low to moderate. Finally, the morphological, physiological, and toxicological variability within D. acuminata may reflect spatial (and/or temporal) population structure, and suggests that sub-specific resolution may be helpful in characterizing bloom dynamics and predicting toxicity.

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“…, as in Raho et al. ), which is similar to the location of the pseudogene insert recovered by Raho et al. () from D. saccula (GenBank Acc.…”

Section: Resultssupporting

confidence: 84%

Section: Discussioncontrasting

confidence: 66%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Characterization and comparison of toxin‐producing isolates of Dinophysis acuminata from New England and Canada

Tong

Smith

Richlen

et al. 2014

Journal of Phycology

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“…Our 100% sequence similarity between P. belizeanum and P. hoffmanianum compares favorably with the results obtained by Raho et al. () where the cob gene was tested to discriminate six Dinophysis species, and it showed very poor resolution. In contrast, Lin et al.…”

Section: Discussionsupporting

confidence: 87%

Are Prorocentrum hoffmannianum and Prorocentrum belizeanum (DINOPHYCEAE, PROROCENTRALES), the same species? An integration of morphological and molecular data

Herrera-Sepúlveda

Medlin

Murugan

et al. 2015

Journal of Phycology

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The taxonomic assignment of Prorocentrum species is based on morphological characteristics; however, morphological variability has been found for several taxa isolated from different geographical regions. In this study, we evaluated species boundaries of Prorocentrum hoffmannianum and Prorocentrum belizeanum based on morphological and molecular data. A detailed morphological analysis was done, concentrating on the periflagellar architecture. Molecular analyses were performed on partial Small Sub-Unit (SSU) rDNA, partial Large Sub-Unit (LSU) rDNA, complete Internal Transcribed Spacer Regions (ITS1-5.8S-ITS2), and partial cytochrome b (cob) sequences. We concatenated the SSU-ITS-LSU fragments and constructed a phylogenetic tree using Bayesian Inference (BI) and maximum likelihood (ML) methods. Morphological analyses indicated that the main characters, such as cell size and number of depressions per valve, normally used to distinguish P. hoffmannianum from P. belizeanum, overlapped. No clear differences were found in the periflagellar area architecture. Prorocentrum hoffmannianum and P. belizeanum were a highly supported monophyletic clade separated into three subclades, which broadly corresponded to the sample collection regions. Subtle morphological overlaps found in cell shape, size, and ornamentation lead us to conclude that P. hoffmanianum and P. belizeanum might be considered conspecific. The molecular data analyses did not separate P. hoffmannianum and P. belizeanum into two morphospecies, and thus, we considered them to be the P. hoffmannianum species complex because their clades are separated by their geographic origin. These geographic and genetically distinct clades could be referred to as ribotypes: (A) Belize, (B) Florida-Cuba, (C1) India, and (C2) Australia.

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Dual quantitative PCR assay for identification and enumeration of Karlodinium veneficum and Karlodinium armiger combined with a simple and rapid DNA extraction method

et al. 2018

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Are the mitochondrial cox1 and cob genes suitable markers for species of Dinophysis Ehrenberg?

Cited by 20 publications

References 46 publications

Characterization and comparison of toxin‐producing isolates of Dinophysis acuminata from New England and Canada

Characterization and comparison of toxin‐producing isolates of Dinophysis acuminata from New England and Canada

Are Prorocentrum hoffmannianum and Prorocentrum belizeanum (DINOPHYCEAE, PROROCENTRALES), the same species? An integration of morphological and molecular data

Dual quantitative PCR assay for identification and enumeration of Karlodinium veneficum and Karlodinium armiger combined with a simple and rapid DNA extraction method

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