Are Root Hydraulic Conductivity Responses to Salinity Controlled by Aquaporins in Broccoli Plants?

López-Berenguer, Carmen; García‐Viguera, Cristina; Carvajal, Micaela

doi:10.1007/s11104-005-7010-x

Cited by 55 publications

(49 citation statements)

References 48 publications

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“…3), was probably caused by closure of the stomata (Robinson et al 1997) or decreased water uptake through the roots. Similar results were reported in pepper (Cabañ ero et al 2004; Martínez-Ballesta -Jiménez et al 2003), and broccoli (Ashraf 2001;Ló pez-Berenguer et al 2006). A reduction in G S could indicate a certain level of acclimation to salinity stress (Munns and Termaat 1986).…”

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confidence: 83%

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The Phi Thickening in Roots of Broccoli Plants: An Acclimation Mechanism to Salinity?

López-Pérez

Fernández‐García

Olmos

et al. 2007

International Journal of Plant Sciences

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Although broccoli is moderately tolerant to salt stress, the tolerance mechanism is still unknown. Therefore, in this article, the uptake and transport of nutrients and water in relation to the changes in root anatomy (phi thickening appearance) caused by salinity stress have been studied. The effect of phi thickening in the response of these plants to salinity was studied by comparing two methods of measuring root hydraulic conductance, pressurizing roots and natural exudation, and analyzing the nutrient concentrations in the xylem. The permeability properties of phi thickening were tested by a tracer that moves only via the apoplastic pathway. Brassica oleracea L. var. Italica plants, grown under different levels of NaCl (0, 40, and 80 mM), showed modifications in the cell wall of the cortical layer bordering the endodermis, such as phi thickenings. The results also showed a decrease of the L 0 and G S of plants under salinity stress and an ionic imbalance in the xylem sap. Na + , Mg 2+ , SO 2À 4 , and Cl À concentrations increased, while Ca 2+ decreased. The fact that the proportion of apoplastic movement, which was higher when plants were measured with the Scholander chamber than with natural exudation, was lower for NaCl-treated plants whereas the alteration of nutrient uptake was similar suggests that the phi thickenings could be a physical barrier only to apoplastic water transport.

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confidence: 83%

“…Ló pez-Berenguer et al (2006) reported a decreased L 0 for broccoli plants 1 wk after applying different concentrations of NaCl. The effect of salinity on the L 0 in other species (Shalhevet et al 1976;Munns and Passioura 1984;Evlagon et al 1990;Azaizeh and Steudle 1991) has been suggested to be due to the osmotic effect of Na + and Cl À .…”

Section: Discussionmentioning

confidence: 97%

The Phi Thickening in Roots of Broccoli Plants: An Acclimation Mechanism to Salinity?

López-Pérez

Fernández‐García

Olmos

et al. 2007

International Journal of Plant Sciences

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“…Alternatively, photosynthetic and growth reductions could have occurred only initially following seedling inoculation with the ECM fungi. Salt inhibits growth and affects water relations and gas exchange in plants (Munns 1993;López-Berenguer et al 2006). In the present study, ECM fungi reduced leaf Na + uptake by elm seedlings when treated with NaCl in non-compacted soil (Fig.…”

Section: Discussionmentioning

confidence: 99%

Responses of ectomycorrhizal American elm (Ulmus americana) seedlings to salinity and soil compaction

2008

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American elm (Ulmus americana) seedlings were either non-inoculated or inoculated with Hebeloma crustuliniforme, Laccaria bicolor and a mixture of the two fungi to study the effects of ectomycorrhizal associations on seedling responses to soil compaction and salinity. The seedlings were grown in the greenhouse in pots containing noncompacted (0.4 g cm −3 bulk density) and compacted (0.6 g cm −3 bulk density) soil and subjected to 60 mM NaCl or 0 mM NaCl (control) treatments for 3 weeks. All three fungal inocula had similar effects on the responses of elm seedlings to soil compaction and salt treatment. In non-compacted soil, ectomycorrhizal fungi reduced plant dry weights, root hydraulic conductance, but did not affect leaf hydraulic conductance and net photosynthesis. When treated with 60 mM NaCl, ectomycorrhizal seedlings had severalfold lower leaf concentrations of Na + compared with the non-inoculated plants. Soil compaction reduced Na + leaf concentrations in non-ectomycorrhizal plants and decreased dry weights, gas exchange and root hydraulic conductance. However, in ectomycorrhizal plants, soil compaction had little effect on the leaf Na + concentrations and on other measured growth and physiological parameters. Our results demonstrated that ECM associations could be highly beneficial to plants growing in sites with compacted soil such as urban areas.

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“…Environmental temperature and humidity were controlled during the experiment. After 5 days, the seedlings were placed in 15 L containers and were supplied with a complete, modified-Hoagland nutrient solution [24]. The solution was replaced every week.…”

Section: Samplesmentioning

confidence: 99%

Supercritical fluid extraction of lipids from broccoli leaves

Arnáiz

Bernal

Martín

et al. 2011

Euro J Lipid Sci & Tech

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The supercritical fluid extraction (SFE) and fractionation of lipids from broccoli leaves is presented in this work. For this purpose the effect of the different variables on the extraction was studied, obtaining the best results at 608C, 300 bar and 3 mL/min. Two different fractions were obtained: First, the samples were extracted with pure CO 2 , and afterward the residual material was extracted using CO 2 modified with 15% of methanol. The total fatty acid content of the extracts was determined by GC-MS and compared with those results obtained by Soxhlet extraction with hexane and a chloroform/methanol (2:1) mixture. The SFE extracts presented a higher percentage of unsaturated fatty acids, especially the polyunsaturated 18:3 n À 3. The methodology was successfully applied to the analysis of the fatty acid composition of the leaves from five different cultivars of broccoli. In all the samples the main fatty acids were a-linolenic (18:3 n À 3), linoleic (18:2 n À 6), and palmitic (16:0). Among the different cultivars analyzed, Naxos variety presented the highest levels in fatty acids, while Parthenon and Viola the lowest. Practical applications:The proposed method allows the fractionation of lipids from broccoli leaves using a small volume of organic solvent and mild conditions. This is advantageous compared to conventional methods where large volumes or organic solvents are used, and the cost and time for the removal of these solvents, along with the possibility of degradation and toxicity, are the major disadvantages. The results obtained contribute to a better compositional characterization and a possible revaluation of this by-product as a source of biologically active compounds.

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Are Root Hydraulic Conductivity Responses to Salinity Controlled by Aquaporins in Broccoli Plants?

Cited by 55 publications

References 48 publications

The Phi Thickening in Roots of Broccoli Plants: An Acclimation Mechanism to Salinity?

The Phi Thickening in Roots of Broccoli Plants: An Acclimation Mechanism to Salinity?

Responses of ectomycorrhizal American elm (Ulmus americana) seedlings to salinity and soil compaction

Supercritical fluid extraction of lipids from broccoli leaves

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