Are pancarcinoma T and Tn differentiation antigens?

Barr, Nancy J.; Taylor, Clive R.; Young, Terrance; Springer, Georg F.

doi:10.1002/1097-0142(19890815)64:4<834::aid-cncr2820640413>3.0.co;2-a

Cited by 41 publications

(25 citation statements)

References 21 publications

(4 reference statements)

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“…The TF antigen is, however, expressed by fetal epithelia and mesothelia (Barr et al 1989). In addition it is also found on transferrin isolated from human amniotic fluid (van Rooijen et al 1998) and produced by the trophoblast (Streu et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Expression of the Thomsen-Friedenreich antigen and of its putative carrier protein mucin 1 in the human placenta and in trophoblast cells in vitro

Jeschke

Richter

Hammer

et al. 2002

Histochem Cell Biol

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The Thomsen-Friedenreich (TF) antigen (or, more precisely, epitope Galbeta1-3GalNAcalpha-O-) has been known for a long time as a carcinoma-associated antigen. In normal tissues the occurrence of TF antigen is restricted to a few immunologically privileged areas. Here we report on the identification of the TF epitope and its putative carrier protein mucin 1 (MUC1) in human placental tissue, on isolated trophoblast cells in vitro and on trophoblast tumour cell lines BeWo and Jeg3. Cryosections of placental and decidual tissues of the first, second and third trimester were double stained with monoclonal antibodies directed against the TF epitope (IgM) and against MUC1 (IgG). In the first trimester of pregnancy we found strong expression of TF antigen and MUC1 at the apical side of the syncytiotrophoblast directed towards the maternal blood. This expression was consistent in the second trimester of pregnancy, and to a lesser degree in the third trimester. In addition, we found positive staining for TF antigen and MUC1 on extravillous trophoblast cells in the decidua during the first and second trimester of pregnancy. Trophoblast tumour cells of the cell line BeWo, which form a syncytium in vitro, were also positive for TF antigen and MUC1, whereas Jeg3 cells, which are unable to form a syncytium, expressed only MUC1. Freshly isolated trophoblast cells from first trimester placentas showed strong staining for MUC1; however, only a few of these cells (less than 1%) were positive for TF antigen, and might consist of digested fragments of the syncytium. In summary, TF antigen and MUC1 are expressed by the syncytiotrophoblast at the feto-maternal interface and by extravillous trophoblast cells invading the decidua, whereas villous cytotrophoblast cells in situ as well as freshly isolated trophoblast cells from first trimester placentas only express MUC1 but not TF antigen.

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Section: Introductionmentioning

confidence: 99%

Expression of the Thomsen-Friedenreich antigen and of its putative carrier protein mucin 1 in the human placenta and in trophoblast cells in vitro

Jeschke

Richter

Hammer

et al. 2002

Histochem Cell Biol

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“…Unmodified Gal␤1-3GalNAc occurs in ϳ90% of all human cancers, including those of the colon, breast, bladder, prostate, liver, ovary, and stomach (56). Besides its remarkable tumor specificity, the Gal␤1-3GalNAc disaccharide is a blood group-related stage-specific oncofetal antigen present during the early fetal phase (5,51,41). It has been shown that Gal␤1-3GalNAc disaccharide and its carrier protein mucin 1 (MUC1) are expressed by the syncytiotrophoblast at the maternal-fetal interface and by extravillous trophoblast cells invading the decidua (20).…”

Section: Discussionmentioning

confidence: 99%

Oxygen governs Galβ1–3GalNAc epitope in human placenta

Ermini

Bhattacharjee

Spagnoletti

et al. 2013

American Journal of Physiology-Cell Physiology

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It is becoming increasingly apparent that the dynamics of glycans reflect the physiological state of cells involved in several cell functions including growth, response to signal molecules, migration, as well as adhesion to, interaction with, and recognition of other cells. The presence of glycoconjugates in human placenta suggests their major role in maternal-fetal exchanges, intercellular adhesion, cellular metabolism, and villous vessel branching. Although several studies have described glycoconjugate distribution in the human placenta descriptions of their physiological function and control mechanisms during placental development are lacking. In this study we investigated the developmental distribution and regulation of placental core 1 O- and N-glycans focusing on early and late first trimester human pregnancy. To define the control mechanisms of the oligosaccharide chains during early placentation process, chorionic villous explants and human trophoblast cell lines were exposed to various oxygen levels. We found that oxygen tension regulates changes in core-1 O-glycan (the disaccharide Galβ1-3GalNAc) epitope expression levels. Moreover, by double affinity chromatography and subsequent analysis with mass spectrometry, we identified in the heat shock protein 90-α (HSP90α) a good candidate as carrier of the Galβ1-3GalNAc epitope at low oxygen tension. Our results support a fundamental role of oxygen tension in modulating glycosylation of proteins during placental development.

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“…In normal adult human tissues TF epitopes are only expressed in limited amounts and are restricted to a few immunologically privileged sites (Cao et al 1996). The TF antigen is, however, expressed on fetal epithelia and mesothelia (Barr et al 1989), on transferrin from human amniotic fluid (van Rooijen et al 1998) and on trophoblast cells (Richter et al 2000). The TF antigen and its carrier protein mucin 1 (MUC1) are expressed by syncytiotrophoblasts at the materno-fetal interphase and by extravillous trophoblast cells invading the decidua.…”

Section: Introductionmentioning

confidence: 99%

Binding of galectin-1 (gal-1) to the Thomsen–Friedenreich (TF) antigen on trophoblast cells and inhibition of proliferation of trophoblast tumor cells in vitro by gal-1 or an anti-TF antibody

Jeschke

Karsten

Wiest

et al. 2006

Histochem Cell Biol

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Galectin-1 (gal-1), a member of the mammalian beta-galactoside-binding proteins, recognizes preferentially Galbeta1-4GlcNAc sequences of several cell surface oligosaccharides. We demonstrate histochemically that the lectin recognizes appropriate glycotopes on the syncytiotrophoblast and extravillous trophoblast layer from second trimester human placenta and on BeWo chorion carcinoma cells. Gal-1 binding to BeWo cells was diminished by the Thomsen-Friedreich (TF)-disaccharide (Galbeta1-3GalNAc-) conjugated to polyacrylamide (TF-PAA). Gal-1 also inhibited BeWo cell proliferation in a concentration-dependent manner. Similar antiproliferative effects were also observed with an anti-TF monoclonal antibody (mAb, A78-G/A7). Therefore, we conclude that ligation of Galbeta1-4GlcNAc and Galbeta1-3GalNAc epitopes on BeWo cells may have regulatory effects on cell proliferation.

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Are pancarcinoma T and Tn differentiation antigens?

Cited by 41 publications

References 21 publications

Expression of the Thomsen-Friedenreich antigen and of its putative carrier protein mucin 1 in the human placenta and in trophoblast cells in vitro

Expression of the Thomsen-Friedenreich antigen and of its putative carrier protein mucin 1 in the human placenta and in trophoblast cells in vitro

Oxygen governs Galβ1–3GalNAc epitope in human placenta

Binding of galectin-1 (gal-1) to the Thomsen–Friedenreich (TF) antigen on trophoblast cells and inhibition of proliferation of trophoblast tumor cells in vitro by gal-1 or an anti-TF antibody

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