Are ovine and porcine carotid arteries equivalent animal models for experimental cardiac surgery: A quantitative histological comparison

Grajciarová, Martina; Turek, Daniel; Malečková, Anna; Pálek, Richard; Liška, Václav; Tomášek, Petr; Králíčková, Milena; Tonar, Zbyněk

doi:10.1016/j.aanat.2022.151910

Cited by 5 publications

(2 citation statements)

References 67 publications

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“…The design of the quantitative analysis was based on previously published studies on various vessels morphology [40][41][42]. The area fractions of elastin, collagen and smooth muscle actin were quanti ed using the stereological point grid and Cavalieri principle [43].…”

Section: Stereological Quanti Cationmentioning

confidence: 99%

“…The fractions were estimated as the ratio of the evaluated microscopic structures to the reference area. The settings of the used point grid were in agreement with the standards of stereological methods [44].The two-dimensional densities of nuclear pro les, vascular pro les and MAC387-positive cells were evaluated using the unbiased counting frame [40,45]. These densities were estimated as the number of the evaluated structures per reference area.…”

Section: Stereological Quanti Cationmentioning

confidence: 99%

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Decellularized porcine vena cava grafts are fully repopulated after orthotopic implantaiton

Palek,

Massaro,

Cervenkova

et al. 2024

Preprint

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Tissue-engineered organs, based on native extracellular matrix (ECM) scaffolds, could be a game changer in regenerative medicine applications. Decellularization technology provides such scaffolds with organ-typic ECM composition and architecture. Despite limitations such as the requirement of huge cell numbers and finding the optimal route of entry, recellularized scaffolds provide alternative grafts for transplantation. In this study we assessed whether decellularized scaffolds, when implanted, are repopulated from the adjacent tissue. Since the vasculature plays an important role in tissue functionality, our main focus was to evaluate in situ repopulation of decellularized veins in a pig model. For this, porcine inferior vena cava grafts were decellularized and orthotopically implanted in recipient pigs (n=12). To evaluate possible immune responses to the scaffolds and to assess potential thrombus formation, cellular allogeneic vena cava grafts were transplanted in control pigs (n=8). Within 28 days after implantation, the decellularized veins were fully recellularized with endothelial cells and smooth muscle cells. Quantitative histological analysis showed a comparable amount of smooth muscle actin in the repopulated decellularized grafts similar to the native IVC. Lymphocyte infiltrates representing signs of graft rejection were not detected in the pigs, as opposed to the control group that received the allogeneic grafts. The decellularized grafts provoked a higher incidence of thrombosis in comparison with allogeneic grafts (33.3 vs. 12.5%). With this study, we show efficient in situ repopulation of decellularized vein grafts. These findings are insightful and promising to further explore the use of decellularized tissue without the need for full pre-transplant recellularization.

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Section: Stereological Quanti Cationmentioning

confidence: 99%

Section: Stereological Quanti Cationmentioning

confidence: 99%

Decellularized porcine vena cava grafts are fully repopulated after orthotopic implantaiton

Palek,

Massaro,

Cervenkova

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

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Regeneration of Blood Vessels

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Wei,

Midgley

et al. 2023

Polymeric Biomaterials for Tissue Regeneration

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Decellularized porcine vena cava grafts are fully repopulated after orthotopic implantation

Palek,

Massaro,

Cervenkova

et al. 2024

Discov Appl Sci

View full text Add to dashboard Cite

Tissue-engineered organs, based on native extracellular matrix (ECM) scaffolds, could be a game changer in regenerative medicine applications. Decellularization technology provides such scaffolds with organ-typic ECM composition and architecture. Despite limitations such as the requirement of huge cell numbers and finding the optimal route of entry, recellularized scaffolds provide alternative grafts for transplantation. In this study we assessed whether decellularized scaffolds, when implanted, are repopulated from the adjacent tissue. Since the vasculature plays an important role in tissue functionality, our main focus was to evaluate in situ repopulation of decellularized veins in a pig model. For this, porcine inferior vena cava grafts were decellularized and orthotopically implanted in recipient pigs (n = 12). To evaluate possible immune responses to the scaffolds and to assess potential thrombus formation, cellular allogeneic vena cava grafts were transplanted in control pigs (n = 8). Within 28 days after implantation, the decellularized veins were fully recellularized with endothelial cells and smooth muscle cells. Quantitative histological analysis showed a comparable amount of smooth muscle actin in the repopulated decellularized grafts similar to the native IVC. Lymphocyte infiltrates representing signs of graft rejection were not detected in the pigs, as opposed to the control group that received the allogeneic grafts. The decellularized grafts provoked a higher incidence of thrombosis in comparison with allogeneic grafts (33.3 vs. 12.5%). With this study, we show efficient in situ repopulation of decellularized vein grafts. These findings are insightful and promising to further explore the use of decellularized tissue without the need for full pre-transplant recellularization. Graphical abstract

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Are ovine and porcine carotid arteries equivalent animal models for experimental cardiac surgery: A quantitative histological comparison

Cited by 5 publications

References 67 publications

Decellularized porcine vena cava grafts are fully repopulated after orthotopic implantaiton

Decellularized porcine vena cava grafts are fully repopulated after orthotopic implantaiton

Regeneration of Blood Vessels

Decellularized porcine vena cava grafts are fully repopulated after orthotopic implantation

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