Are neutralizing anti–GM-CSF autoantibodies present in all healthy persons?

Meager, Anthony; Cludts, Isabelle; Thorpe, Robin; Wadhwa, Meenu

doi:10.1182/blood-2009-08-241018

Cited by 12 publications

(4 citation statements)

References 13 publications

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“…However, other studies found GM-CSF autoantibodies only in the serum of patients with pulmonary alveolar proteinosis or myasthenia gravis, but none or a very low percentage was found in healthy controls [61–64]. These different results may reflect differences in assay design, and the specificity of the detection methods [65]. Importantly, increased levels of GM-CSF autoantibodies have been found also in pediatric and adult patients with CD with ileal or ileocolonic involvement, compared with those with colon involvement only, UC patients and healthy controls.…”

Section: Gm-csf In Gastrointestinal Diseasementioning

confidence: 99%

GM-CSF: a role in immune and inflammatory reactions in the intestine

Egea

Hirata

Kagnoff

2010

Expert Review of Gastroenterology & Hepatology

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Granulocyte macrophage colony-stimulating factor (GM-CSF) is a cytokine that promotes myeloid cell development and maturation, and dendritic cell differentiation and survival in vitro. Growing evidence supports the notion that GM-CSF has a major role in some inflammatory and autoimmune reactions and in the host’s response to pulmonary infection, but few studies have addressed its functions and importance in the GI tract. Recent studies demonstrated that administration of GM-CSF can result in clinical improvement in patients with Crohn’s disease. Mice deficient in GM-CSF (GM-CSF−/−) developed more severe intestinal and systemic infection after an enteric infection, and more severe colitis in response to enteric exposure to dextran sodium sulfate. Both the severity of infection and colitis were largely prevented by GM-CSF administration. Such studies indicate that GM-CSF has an important role in the regulation of intestinal immune and inflammatory responses.

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Section: Gm-csf In Gastrointestinal Diseasementioning

confidence: 99%

GM-CSF: a role in immune and inflammatory reactions in the intestine

Egea

Hirata

Kagnoff

2010

Expert Review of Gastroenterology & Hepatology

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“…Prelavage and postlavage CT scans showed a decrease in density, which would be expected secondary to clearing of the PAS-positive material (figure 1F, G). In a few cases, GM-CSF therapy has been used,37 38 and the overall response rate appears nearly 50%; this is in contrast to the ability of WLL to produce rapid improvements in symptoms, radiological images and lung function in all patients 17 19 26. GM-CFS therapy was not considered in the reference centre where anti-GM-CSF testing was performed.…”

Section: Discussionmentioning

confidence: 99%

Autoimmune pulmonary proteinosis in a Chilean teenager, a rare aetiology of interstitial lung disease

Strickler¹,

Boza

Koppmann

et al. 2014

BMJ Case Reports

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Interstitial lung disease (ILD) is rare and encompasses a heterogeneous group of diseases, and is even rarer in children than in adults. ILDs compromise more than 100 different entities, including pulmonary alveolar proteinosis (PAP). There are many causes of PAP in children, including surfactant protein gene mutations (SFTPB, SFTPC, ABCA3, TTF-1), GMCSF receptor mutations and antigranulocyte-macrophage colony-stimulating factor autoantibodies. We report a case of a 13-year-old Chilean girl who presented with an 8-month history of progressive exercise intolerance, fatigability and diminished school performance. Physical examination revealed resting tachypnoea, a few basal bilateral inspiratory crackles, and hypoxaemia on minimal exertion. Clinical suspicion and evaluation, including international collaboration, led to the diagnosis of autoimmune PAP and specific therapy for the condition.

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“…In that study, we did not mention about nonspecific binding of IgG other than GMAbs with GM-CSF coated on the ELISA plate. In this regard, Meager et al submitted a criticism to our report that nonspecific binding of IgG other than GMAbs might affect the results of measuring GMAbs concentrations in the sera of normal subjects [12]. According to their criticism, we evaluated the nonspecific binding of IgG other than GMAbs.…”

Section: Discussionmentioning

confidence: 99%

Estimation of the True Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Autoantibody Concentration in the Sera of Normal Subjects

Shiiya¹,

Iizuka²,

Ito³

et al. 2017

J Bioanal Biomed

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According to our previous studies, Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) autoantibodies (GMAbs) are normally present in healthy people. To date, antigen-antibody capture assay has measured the concentrations. However, nonspecific IgG other than GMAbs and immune complex formation with intrinsic GM-CSF prevents us from measuring the accurate concentrations. To solve these problems, we developed a liquid phase high-sensitivity assay using biotinylated GM-CSF (bGM-CSF) and streptavidin coated plate, which could measure the concentration of GMAbs over 0.02 ng/ml. Using this method, the true GMAbs concentration in 30 normal subjects was estimated to be 52.6 ng/ml for the median value, ranging 10.6 to 799.5 ng/ml. Thus, we propose a novel method that enables us to estimate the true concentration of GMAbs in the sera of healthy subjects.

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Are neutralizing anti–GM-CSF autoantibodies present in all healthy persons?

Cited by 12 publications

References 13 publications

GM-CSF: a role in immune and inflammatory reactions in the intestine

GM-CSF: a role in immune and inflammatory reactions in the intestine

Autoimmune pulmonary proteinosis in a Chilean teenager, a rare aetiology of interstitial lung disease

Estimation of the True Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Autoantibody Concentration in the Sera of Normal Subjects

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