Are neopolyploids a likely route for a transgene walk to the wild? The Aegilops ovata × Triticum turgidum durum case

David, Jacques; Benavente, Elena; Bres-Patry, Cécile; Dusautoir, Jean-Claude; Echaide, Mercedes

doi:10.1111/j.1095-8312.2004.00336.x

Cited by 29 publications

(33 citation statements)

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“…crassa (2n = 42, DDDDMM), Ae. triuncialis (2n = 28, UUCC), Hynaldia villosa (2n = 14, VV) and rye (Secale cereale L., 2n = 14, RR) as well as in haploid plants of T. t. durum (Blanco et al 1983;Stefani et al 1983;Liu et al 1986;Xu and Dong 1992;Balatero and Darvey 1993;Pignone 1993;Xu and Joppa 2000;Jauhar et al 2000;David et al 2004). Obviously, meiotic restitution usually takes place in a plant with a haploid genome as a result of failure of either the first or the second meiotic division.…”

Section: Introductionmentioning

confidence: 99%

Meiotic restriction in emmer wheat is controlled by one or more nuclear genes that continue to function in derived lines

et al. 2007

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Highly fertile F 1 hybrids were made between Triticum turgidum L. ssp. turgidum (2n = 28, AABB) and Aegilops tauschii Coss. (2n = 14, DD) without embryo rescue and hormone treatment. The F 1 plants had an average seed set of 25%. Approximately 96% of the F 2 seeds were able to germinate normally and about 67% of the F 2 plants were spontaneous amphidiploid (2n = 42, AABBDD). Cytological analysis of male gametogenesis of the F 1 plants showed that meiotic restitution is responsible for the high fertility. A mitosis-like meiosis led to meiotic restitution at either of the two meiotic divisions resulting in unreduced gametes. Test crosses of the T. t. turgidum-Ae. tauschii amphidiploid with Ae. variabilis and rye suggested that the mitosis-like meiosis is controlled by one or more nuclear genes that continue to function in derived lines. This discovery indicates a potential application of such genes in producing double haploids.

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Section: Introductionmentioning

confidence: 99%

Meiotic restriction in emmer wheat is controlled by one or more nuclear genes that continue to function in derived lines

et al. 2007

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“…Aegilops hybrids are highly sterile, but descendents can be found after back-crossing to any of the parents or spontaneous amphiploidy. Thus, these hybrid-derived forms are acknowledged as the most likely bridges for wheat gene flow in nature [David et al, 2004].…”

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A Comparative Analysis of Chromosome Pairing at Metaphase I in Interspecific Hybrids between Durum Wheat (Triticum turgidum L.) and the Most Widespread Aegilops Species

Cifuentes

Garcia-Agüero

Benavente

2010

Cytogenet Genome Res

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Homoeologous metaphase I (MI) associations in hybrids between durum wheat and its wild allotetraploid relatives Aegilops neglecta, Ae. triuncialis and Ae. ventricosa have been characterized by a genomic in situ hybridization procedure that allows simultaneous discrimination of A, B and wild species genomes. Earlier results in equivalent hybrids with the wild species Ae. cylindrica and Ae. geniculata have also been considered to comparatively assay the MI pairing pattern of the durum wheat × Aegilops interspecific combinations more likely to occur in nature. The general picture can be drawn as follows. A and B wheat genomes pair with each other less than the 2 wild constituent genomes do in any of the hybrid combinations examined. Interspecific wheat-wild associations account for 60–70% of total MI pairing in all hybrids, except in that derived from Ae. triuncialis, but the A genome is always the wheat partner most frequently involved in MI pairing with the wild homoeologues. Hybrids with Ae. cylindrica, Ae. geniculata and Ae. ventricosa showed similar reduced levels of MI association and virtually identical MI pairing patterns. However, certain recurrent differences were found when the pattern of homoeologous pairing of hybrids from either Ae. triuncialis or Ae. neglecta was contrasted to that observed in the other durum wheat hybrid combinations. In the former case, a remarkable preferential pairing between the wild species constituent genomes U^t and C^t seems to be the reason, whereas a general promotion of homoeologous pairing, qualitatively similar to that observed under the effect of the ph1c mutation, appears to occur in the hybrid with Ae. neglecta. It is further discussed whether the results reported here can be extrapolated to the corresponding bread wheat hybrid combinations.

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“…There is clear evidence that viable seeds are produced by functional 2n gametes in durum wheat haploids (Jauhar et al, 2000;Jauhar, 2003). Similarly, David et al (2004) observed that fertile Ae. geniculata × T. turgidum durum hybrids always produced total or partial amphiploids in their offspring.…”

Section: Discussionmentioning

confidence: 97%

“…In the amphiploids every chromosome of the original hybrids is represented twice and each chromosome has a homologous partner to pair at meiosis. Thus, meiosis is regular and fertile derivatives can be obtained from hybrids whose sterility was caused by chromosomal unbalance and irregularity (Bretagnolle and Thompson, 1995;Ramsey and Schemske, 1998;David et al, 2004). In their review on hybridization between wheat and its relatives, Zaharieva and Monneveux (2006) mentioned that Tschermak and Bleier (1926) were the first to obtain an amphiploid species as a result of the spontaneous doubling of the chromosomes of a wheat hybrid produced by the cross of T. turgidum dicoccoides with Ae.…”

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Spontaneous wheat-Aegilops biuncialis, Ae. geniculata and Ae. triuncialis amphiploid production, a potential way of gene transference

Loureiro

Escorial

García-Baudín

et al. 2009

Span. j. agric. res.

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Some F1 hybrid plants between three species of the Aegilops genus and different hexaploid wheat Triticum aestivum cultivars show certain self-fertility, with averages of F1 hybrids bearing F2 seeds of 8.17%, 5.12% and 48.14% for Aegilops biuncialis, Aegilops geniculata and Aegilops triuncialis respectively. In the Ae. triuncialis-wheat combination with ";Astral" wheat cultivar, the fertility was higher than that found in the other combinations. All the F2 seeds studied were spontaneous amphiploids (2n=10x=70). The present study evidences the possibility of spontaneous formation of amphiploids between these three Aegilops species and hexaploid wheat and discusses their relevance for gene transference. Future risk assessment of transgenic wheat cultivars needs to evaluate the importance of amphiploids as a bridge for transgene introgression and for gene escape to the wild.

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Are neopolyploids a likely route for a transgene walk to the wild? The Aegilops ovata × Triticum turgidum durum case

Cited by 29 publications

References 34 publications

Meiotic restriction in emmer wheat is controlled by one or more nuclear genes that continue to function in derived lines

Meiotic restriction in emmer wheat is controlled by one or more nuclear genes that continue to function in derived lines

A Comparative Analysis of Chromosome Pairing at Metaphase I in Interspecific Hybrids between Durum Wheat <i>(Triticum turgidum </i>L<i>.)</i> and the Most Widespread <i>Aegilops</i> Species

Spontaneous wheat-Aegilops biuncialis, Ae. geniculata and Ae. triuncialis amphiploid production, a potential way of gene transference

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