Are Metabolic Disorders in Hypertensive Patients Associated With Gnb3 (Rs5443) Genetic Polymorphisms?

Abstract

Objective:Primary/essential hypertension (PH) is the most common cause of left ventricular hypertrophy (LVH) and is often associated with metabolic disorders. The aim of the study was to analyse dependence of metabolic parameters on the guanine nucleotide binding protein (G-protein) 3 subunit gene polymorphism (GN3, rs5443) in hypertensive patients.Design and method:A cross-sectional study involved 72 PH patients of high and very high cardiovascular risk (29.16% men and 70.84% women); the average age 59.87 ± 7.98 y. The control group included 48 healthy individuals (aged 49.13 ± 6.28 y) and sex distribution (62.5% women and 37.5% men). GN3 (C825T) polymorphism was investigated by Real Time PCR. LVH was assessed by EchoKG. Metabolic disorders were studied by lipids panel (Total cholesterol (TC), Triglycerides (TG), Low-, and High- density lipoprotein cholesterol (LDL-C, HDL-C) levels) and Glucose blood value. The atherogenic index (AI) was calculated by the formula: (TC – HDL-C)/ HDL-C.Results:In CC-genotype carriers of the GN3 gene metabolic parameters were as follows: TC 5.50 ± 0.79 mmol/L, TG 2.10 ± 0.8 mmol/L, HDL-C 1.22 ± 0.22 mmol/L, LDL-C 4.03 ± 0.76 mmol/L, AI 3.66 ± 0.84 U, Glucose 7.7 ± 2.34 mmol/L. In PH patients with TC-genotype the concentration of TC was 5.82 ± 1.15 mmol/L (pCC>0.05), TG 1.73 ± 0.55 mmol/L (pCC> 0.05), HDL-C 1.30 ± 0.21mmol/L (pCC> 0.05); LDL-C 4.39 ± 1.07 mmol/L (pCC> 0.05), AI 3.61 ± 0.95 (pCC> 0.05), Glucose 7.37 ± 2.34 mmol/L (pCC> 0.05). In TT-genotype carriers the concentration of TC was 6.6 ± 0.64 mmol/L (it was higher than in C-allelle carriers by 20.0% (pCC> 0.05) and 13.79% (pTC = 0.016) as much), TG – 2.6 ± 1.27 mmol/L, that was higher than in C-allelle patients by 23.81% (pCC> 0.05) and 52.94% (pTC = 0.038), respectively. The other parameters did not differed significantly between genotypes’ carriers and in mutation homozygous T-allele carriers were as follows: HDL-C 1.3 ± 0.05 mmol/L (pCC, TC> 0.05), LDL-C 4.7 ± 0.69 mmol/L (pCC, TC> 0.05), AI 4.0 ± 0.69 (pCC, TC> 0.05), Glucose 6.20 ± 1.2 mmol/L (pCC,TC> 0.05).Conclusions:Therefore, the metabolic disorders in hypertensive patients do not depend on the GN3 (rs5443) gene polymorphism.