Are lithium niobate (LiNbO3) and lithium tantalate (LiTaO3) ferroelectrics bioactive?

Vilarinho, Paula M.; Barroca, Nathalie; Złotnik, Sebastian; Félix, Pedro Tiago Albergaria; Fernandes, Maria Helena

doi:10.1016/j.msec.2014.03.026

Cited by 42 publications

(22 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other works deal with the bioactivity of LN and LT powders, but without any attention to the adhesion and morphology of the cells onto the crystal surface. 30 Conversely, different papers have been published in the past several years about the adhesion of osteoblastic cells to polarized ceramic substrates. 15,21−24 Therefore, today the literature lacks publications concerning the influence of the ferroelectric polarization on cell adhesion and morphology.…”

Section: ■ Discussionmentioning

confidence: 99%

Effects of Lithium Niobate Polarization on Cell Adhesion and Morphology

Marchesano

Gennari

Mecozzi

et al. 2015

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Understanding how the interfacial effects influence cell adhesion and morphology is of fundamental interest for controlling function, growth, and movement of cells in vitro and in vivo. In particular, the influence of surface charges is well-known but still controversial, especially when new functional materials and methods are introduced. Here, the influence of the spontaneous polarization of ferroelectric lithium niobate (LN) on the adhesion properties of fibroblast cells is investigated. The spontaneous polarization of LN has one of the largest known magnitudes at room temperature (∼78 μC/cm(2)), and its orientation can be patterned easily by an external voltage, this motivating highly the investigation of its interaction with cells. Immunofluorescence and migration assays show strong evidence that the surface polarity regulates the adhesion functions, with enhanced spreading of the cytoskeleton on the negative face. The results suggest the potential of LN as a platform for investigating the role of charges on cellular processes, thus favoring new strategies in fabricating those biocompatible constructs used for tissue engineering. In fact, the orientation of the high-magnitude polarization can be patterned easily and, in combination with piezoelectric, pyroelectric, and photorefractive properties, may open the route to more sophisticated charge templates for modulating the cell response.

show abstract

Section: ■ Discussionmentioning

confidence: 99%

Effects of Lithium Niobate Polarization on Cell Adhesion and Morphology

Marchesano

Gennari

Mecozzi

et al. 2015

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…The mechanism of cell adhesion and the resulting morphology on different surfaces is complex, often dependent on a wide range of factors such as the protein species adsorbed on the surfaces [ 16 , 17 ], surface structure geometries [ 17 , 18 , 19 , 20 , 21 ], roughness [ 22 , 23 , 24 , 25 , 26 , 27 ], and surface energy of the substrata [ 22 , 28 ]. Recently, novel functional biocompatible ferroelectric materials, such as lithium niobate and lithium tantalate, have been used to manipulate cell behavior [ 29 , 30 , 31 , 32 , 33 , 34 , 35 ]. In particular, the surface charge of these materials is able to enhance osteoblast function, mineral formation [ 31 ], and create human neuroblastoma cell patterns [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Nanoscale-Textured Tantalum Surfaces for Mammalian Cell Alignment

et al. 2018

View full text Add to dashboard Cite

Tantalum is one of the most important biomaterials used for surgical implant devices. However, little knowledge exists about how nanoscale-textured tantalum surfaces affect cell morphology. Mammalian (Vero) cell morphology on tantalum-coated comb structures was studied using high-resolution scanning electron microscopy and fluorescence microscopy. These structures contained parallel lines and trenches with equal widths in the range of 0.18 to 100 μm. Results showed that as much as 77% of adherent cell nuclei oriented within 10° of the line axes when deposited on comb structures with widths smaller than 10 μm. However, less than 20% of cells exhibited the same alignment performance on blanket tantalum films or structures with line widths larger than 50 μm. Two types of line-width-dependent cell morphology were observed. When line widths were smaller than 0.5 μm, nanometer-scale pseudopodia bridged across trench gaps without contacting the bottom surfaces. In contrast, pseudopodia structures covered the entire trench sidewalls and the trench bottom surfaces of comb structures with line-widths larger than 0.5 μm. Furthermore, results showed that when a single cell simultaneously adhered to multiple surface structures, the portion of the cell contacting each surface reflected the type of morphology observed for cells individually contacting the surfaces.

show abstract

“…However, the angiogenic effects of Li + released from biomaterials have not yet been assessed. The studies that have examined the effect of the addition of Li + into different biomaterials have focused on the evaluation of the physicochemical and structural properties as well as on the osteogenic potential of some of them [ 18 , 19 , 22 , 23 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 ]. In particular, the incorporation of Li + into bioactive glasses was first described by Khorami et al, who partially substituted Na 2 O by variable amounts of Li 2 O (3, 7, and 12 wt %) in a bioactive glass in the SiO 2 -CaO-Na 2 O-P 2 O 5 (45S5) system [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Human Umbilical Vein Endothelial Cells Response to Ionic Dissolution Products from Lithium-Containing 45S5 Bioactive Glass

et al. 2017

View full text Add to dashboard Cite

Since lithium (Li+) plays roles in angiogenesis, the localized and controlled release of Li+ ions from bioactive glasses (BGs) represents a promising alternative therapy for the regeneration and repair of tissues with a high degree of vascularization. Here, microparticles from a base 45S5 BG composition containing (wt %) 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5, in which Na2O was partially substituted by 5% Li2O (45S5.5Li), were obtained. The results demonstrate that human umbilical vein endothelial cells (HUVECs) have greater migratory and proliferative response and ability to form tubules in vitro after stimulation with the ionic dissolution products (IDPs) of the 45S5.5Li BG. The results also show the activation of the canonical Wnt/β-catenin pathway and the increase in expression of proangiogenic cytokines insulin like growth factor 1 (IGF1) and transforming growth factor beta (TGFβ). We conclude that the IDPs of 45S5.5Li BG would act as useful inorganic agents to improve tissue repair and regeneration, ultimately stimulating HUVECs behavior in the absence of exogenous growth factors.

show abstract

Are lithium niobate (LiNbO3) and lithium tantalate (LiTaO3) ferroelectrics bioactive?

Cited by 42 publications

References 24 publications

Effects of Lithium Niobate Polarization on Cell Adhesion and Morphology

Effects of Lithium Niobate Polarization on Cell Adhesion and Morphology

Nanoscale-Textured Tantalum Surfaces for Mammalian Cell Alignment

In Vitro Human Umbilical Vein Endothelial Cells Response to Ionic Dissolution Products from Lithium-Containing 45S5 Bioactive Glass

Contact Info

Product

Resources

About