Are chondrocytes damaged when rheumatologic inflammation is suppressed?

Güzelant, Aliye Yıldırım; Isyar, Mehmet; Yılmaz, İbrahim; Şirin, Duygu Yaşar; Çakmak, Selami; Mahiroğulları, Mahir

doi:10.3109/01480545.2016.1166249

Cited by 15 publications

(39 citation statements)

References 20 publications

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“…However, results are not transferable to human studies because of differences in physiological structure and sensitivity (15,16). Another study was performed with commercial cell lines rather than animal tissues (31).…”

Section: Discussionmentioning

confidence: 99%

Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue

Karaarslan

Batmaz²,

Yılmaz

et al. 2018

Exp Ther Med

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Non-steroidal anti-inflammatory drugs (NSAIDs) that are applied through oral, injectable or topical routes have been widely used in painful and inflammatory musculoskeletal diseases. The current study aimed to determine whether naproxen, an aryl acetic acid derivative with analgesic and anti-inflammatory effects, has a toxic effect on human chondrocytes. Samples containing monolayer primary chondrocyte cultures were prepared following resection from osteochondral tissues obtained from patients with gonarthrosis. Cell viability, toxicity and proliferation and levels of stage-specific embryonic antigen-1, a precursor to human prechondrocytes, were evaluated spectrophotometrically. The results from the untreated control group were compared with those of the study groups, where naproxen was administered in varying doses (1–1,000 µM). Surface morphologies of the cells were compared using inverted light and environmental scanning electron microscopy. Treatment groups were compared by analysis of variance with Tukey's honest difference post hoc test. P<0.01 was considered to indicate a statistically significant difference. The research revealed significant changes to proliferation and differentiation of chondrocytes in all treatment groups (P<0.01). Naproxen was demonstrated to suppress chondrocyte proliferation and differentiation, which may be an important factor to consider when prescribing this medication to patients.

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Section: Discussionmentioning

confidence: 99%

Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue

Karaarslan

Batmaz²,

Yılmaz

et al. 2018

Exp Ther Med

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“…From the point of view of clinical practice, effects of drugs are divided into two categories, desired effects and adverse effects. Most adverse effects are harmful regardless of the drug's indications of drugs, and these effects are called toxic (4,8,(26)(27)(28)(29)(30)(31).…”

Section: Discussionmentioning

confidence: 99%

“…Drug toxicity is a serious complication in treatments (4,8,(26)(27)(28)(29)(30)(31). these toxicities may result in dosage increases, delays, termination of treatments, or death.…”

Section: Discussionmentioning

confidence: 99%

“…these toxicities may result in dosage increases, delays, termination of treatments, or death. Increases in preventive treatment and developments in supportive care contribute to improvements and favorable results in treatments because the prime purpose is to provide the desired benefits with less toxicity (4,8,(26)(27)(28)(29)(30)(31).…”

Section: Discussionmentioning

confidence: 99%

“…A drug that is toxic to one person may not be toxic to another. In other words, reactions may occur with use of a drug that may differ from person to person, and some people may have severe reactions (4,8,(24)(25)(26)(27)(28)(29)(30)(31). For instance, in some studies, cigarette and alcohol users were more sensitive than non-smokers to the bronchoconstrictor effects of beta-blockers (35,36).…”

Section: Discussionmentioning

confidence: 99%

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Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?

Karaarslan¹,

Yılmaz²,

Şirin³

et al. 2018

J Turgut Ozal Med Cent

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Aim: Nimodipine is used to prevent cerebrovascular-originated ischemic neurological deficits, yet its effects on nucleus pulposus (NP) cells or annulus fibrosus (AF) cells weren't studied. This study aimed to examine nimodipine's effects on vitality and proliferation of chondroadherin (CHAD), type II collagen (COL2A1), and hypoxia-inducible factor 1 alpha (HIF 1α) gene expression in human primary NP/AF cells. Material and Methods: NP/AF cell cultures obtained from 6 patients who underwent microdiscectomy were treated with 100 µMolar nimodipine and analyzed at 0, 24, and 48 h. Data were evaluated using one-way ANOVA and post-hoc Tukey HSD with 95% confidence interval. Results: We observed suppressed cell proliferation and increased necrosis in nimodipine-treated NP/AF cell cultures, especially degenerated tissue. COL2A1 gene expression wasn't detected in any experimental groups. CHAD and HIF 1α expression had timedependent decreases in control. CHAD and HIF 1α expression were found to decrease at 24h, but increased at 48h in degenerated tissue. In nimodipine-applied intact tissues, CHAD expression was stable at 24h but 1.62 times higher than control at 48h. HIF 1α levels were lower than control. Conclusion: In nimodipine-treated degenerated AF/NP cultures, CHAD and HIF 1α expressions had time-dependent decreases. However, after complete RT-PCR data evaluation, no correlation between nimodipine application and gene expression occurred.

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The effects of TNF-alpha inhibition on cartilage: a systematic review of preclinical studies

Chisari

Yaghmour

Khan

2020

Osteoarthritis and Cartilage

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Objective: To report the most up-to-date evidence on the effects of tumour necrosis factor (TNF)-alpha inhibition on cartilage with a focus on its clinical relevance. Design: A systematic review was performed by searching PubMed, Embase and Cochrane Library databases. Inclusion criteria were studies of any level of evidence published in peer-reviewed journals reporting clinical or preclinical results written in English. Relative data were extracted and critically analysed. PRISMA guidelines were applied, and risk of bias was assessed as well as the methodological quality of the included studies. Results: 13 studies were included after applying the inclusion and exclusion criteria. Three were in vitro human studies from osteoarthritis (OA) patients. Ten were animal modal studies including two in vitro studies, and eight in vivo studies. TNF-alpha inhibition in in vitro studies was generally reported beneficial due to the improved osteochondral viability, proliferation and chondrogenesis. In addition, TNFalpha inhibition was noted to be beneficial in promoting the natural repair of osteochondral lesions and has a chondroprotective effect in in vivo studies. Conclusion:Based on current evidence, TNF might have the potential to interfere with the healing process of chondral and osteochondral defects occurring naturally or in low inflammatory environment after a cartilage repair procedure. Therefore, the use of biological agents to inhibit its action in cartilage repair surgery could be beneficial, and this could translate into a promising therapy that improves the outcome of currently available cartilage procedures.

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Are chondrocytes damaged when rheumatologic inflammation is suppressed?

Abstract: BAs are generally used during active inflammation, and following the management of inflammation, their dosage should be determined taking into consideration their cellular-level toxic effects on chondrocytes.

Cited by 15 publications

References 20 publications

Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue

Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue

Do we damage nucleus pulposus tissue while treating cerebrovascular ischemic neurological deficits with nimodipine?

The effects of TNF-alpha inhibition on cartilage: a systematic review of preclinical studies

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