Are chitosan–mucin interactions specific to different regions of the stomach? Velocity ultracentrifugation offers a clue

Deacon, Matthew P.; Davis, S.S.; White, Robert J.; Nordman, Henrik; Carlstedt, Ingemar; Errington, Neil; Rowe, Arthur J.; Harding, Stephen E.

doi:10.1016/s0144-8617(98)00097-6

Cited by 41 publications

(16 citation statements)

References 11 publications

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“…Earlier hydrodynamic studies [13,29] have shown a clear effect on mucin-chitosan complexes by increasing the ionic strength. Figure 5 shows the characteristic mucin (Figures 5a and 5c) and complex (Figures 5b and 5d) structures at 0.2 M and 0.3 M ionic strength, respectively.…”

Section: Complexes Of Pgm and Chitosan At 02 M And 03 M Ionic Strengthmentioning

confidence: 94%

“…PGM (sample MD [13]), was purified and isolated according to the modified procedure described in [14]. The purified mucin preparations were gently defrosted and dialysed into buffer overnight at 4 mC before use.…”

Section: Methodsmentioning

confidence: 99%

“…NaCl, [13] were pipetted (20 µl) into an eppendorf. Glycerol solution [15-25 % (v\v)] was then added to a volume of 0.5 ml and left for 30 min.…”

Section: Figure 1 Afm Of the Substrate Mica And Aptes-coated Micamentioning

confidence: 99%

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Atomic force microscopy of gastric mucin and chitosan mucoadhesive systems

Deacon

McGurk

Roberts

et al. 2000

Biochemical Journal

117

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Atomic force microscopy has been utilized to probe, at a molecular level, the interaction between purified pig gastric mucin (PGM) and a mucoadhesive cationic polymer, chitosan (sea cure 210j), with a low degree (approx. 11 %) of acetylation. Images were produced detailing the structures of both PGM and chitosan in 0.1 M acetate buffer (pH 4.5), followed by the complex of the two structures in the same buffer. PGM in 0.1 M acetate buffer revealed long linear filamentous structures, consistent with earlier electron microscopy and scanning tunnelling micoscopy studies. The chitosan molecules also adopted a linear conformation in the same buffer, although with a smaller average

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Section: Complexes Of Pgm and Chitosan At 02 M And 03 M Ionic Strengthmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

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Atomic force microscopy of gastric mucin and chitosan mucoadhesive systems

Deacon

McGurk

Roberts

et al. 2000

Biochemical Journal

117

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“…[61] When hydroxypropylmethylcellulose (HPMC) was added to mucin dispersion instead of chitosans and Carbopols, the zeta-potential of particles remained unchanged, indicating poor mucoadhesive affinity of this non-ionic polysaccharide. [61] In a series of publications Harding and coworkers [69][70][71][72] have reported the use of analytical centrifugation to probe mucoadhesive interactions between freshly isolated mucins and various polymers in solution mixtures. They demonstrated that interactions between mucins and mucoadhesive polymers result in the changes of the sedimentation velocity and molecular weights due to the formation of large complex aggregates.…”

Section: Methods To Study Mucoadhesive Interactionsmentioning

confidence: 99%

Advances in Mucoadhesion and Mucoadhesive Polymers

Khutoryanskiy

2010

Macromolecular Bioscience

504

421

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Mucoadhesion is the ability of materials to adhere to mucosal membranes in the human body and provide a temporary retention. This property has been widely used to develop polymeric dosage forms for buccal, oral, nasal, ocular and vaginal drug delivery. Excellent mucoadhesive properties are typical for hydrophilic polymers possessing charged groups and/or non-ionic functional groups capable of forming hydrogen bonds with mucosal surfaces. This feature article considers recent advances in the study of mucoadhesion and mucoadhesive polymers. It provides an overview on the structure of mucosal membranes, properties of mucus gels and the nature of mucoadhesion. It describes the most common methods to evaluate mucoadhesive properties of various dosage forms and discusses the main classes of mucoadhesives.

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“…However, other separation systems are now available. such as 'field flow fractionation' (FFF) and work is currently in progress to monitor the effectiveness of these systems for the characterisation of mucoadhesives (Deacon, 1999; …”

Section: Viscometry and Rheologymentioning

confidence: 99%