Arctic antibiotic resistance gene contamination, a result of anthropogenic activities and natural origin

Tan, Lu; Li, Linyun; Ashbolt, Nicholas J.; Wang, Xiaolong; Cui, Yuxiao; Zhu, Xiao; Xu, Yan; Yang, Yang; Mao, Daqing; Luo, Yi

doi:10.1016/j.scitotenv.2017.10.110

Cited by 103 publications

(33 citation statements)

References 82 publications

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“…The increasing ARGs have been recognized as a consequence of the massive use of antibiotics in therapeutics and agriculture (Levy and Marshall, 2004; Huerta et al, 2013). However, more evidence shows that the dissemination of ARGs can also be influenced by heavy metal contamination (Pruden et al, 2006; Knapp et al, 2017; Tan et al, 2018). As early as in the 1970s, it was found that heavy metal resistance and antibiotic resistance can be selected simultaneously in the heavy metal contaminant ecosystem (Timoney et al, 1978).…”

Section: Introductionmentioning

confidence: 99%

Bacterial Heavy-Metal and Antibiotic Resistance Genes in a Copper Tailing Dam Area in Northern China

et al. 2019

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Heavy metal resistance genes (MRGs) and antibiotic resistance genes (ARGs) in bacteria can respond to the inducement of heavy metals. However, the co-occurrence of MRGs and ARGs in the long-term heavy metal contaminated area is still poorly understood. Here, we investigated the relationship between the abundance of soil bacteria MRGs, ARGs and heavy metal pollution in a copper tailing dam area of northern China. We found that ars C and ere A genes coding for resistance mechanisms to arsenic and to macrolides, respectively, are the most abundant MRG and ARG in the study area. The abundance of MRGs is positively correlated with cadmium (Cd) concentration, and this indicates the importance of Cd in the selection of MRGs. The network analysis results show that sul II and MRGs co-occur and cop B occur with ARGs, which suggests that MRGs and ARGs can be co-selected in the soil contaminated by heavy metal. The network analysis also reveals the co-occurrence of Cd and MRGs, and thus heavy metal with a high ‘toxic-response’ factor can be used as the indicator of MRGs. This study improves the understanding of the relationship between bacterial resistance and multi-metal contamination, and underlies the exploration of the adaptive mechanism of microbes in the multi-metal contaminated environment.

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Section: Introductionmentioning

confidence: 99%

Bacterial Heavy-Metal and Antibiotic Resistance Genes in a Copper Tailing Dam Area in Northern China

et al. 2019

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“…In addition to the antibiotic contamination of water bodies, which exerts a selective pressure on aquatic microbial communities, their use in the aquaculture industry causes the accumulation of antibiotic residues in fish and shellfish tissues, which in turn selects for resistances in the fish microbiota [ 6 ]. Although the presence of antibiotics, and consequently ARGs, in aquatic environments subjected to a high anthropogenic impact is well established [ 7 ], there is less information available about ARGs in areas with few human activities, such as polar aquatic environments, where ARGs have been detected [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems

et al. 2020

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Anthropogenic activities are a key factor in the development of antibiotic resistance in bacteria, a growing problem worldwide. Nevertheless, antibiotics and resistances were being generated by bacterial communities long before their discovery by humankind, and might occur in areas without human influence. Bacteriophages are known to play a relevant role in the dissemination of antibiotic resistance genes (ARGs) in aquatic environments. In this study, five ARGs (blaTEM, blaCTX-M-1, blaCTX-M-9, sul1 and tetW) were monitored in phage particles isolated from seawater of two different locations: (i) the Mediterranean coast, subjected to high anthropogenic pressure, and (ii) the Antarctic coast, where the anthropogenic impact is low. Although found in lower quantities, ARG-containing phage particles were more prevalent among the Antarctic than the Mediterranean seawater samples and Antarctic bacterial communities were confirmed as their source. In the Mediterranean area, ARG-containing phages from anthropogenic fecal pollution might allow ARG transmission through the food chain. ARGs were detected in phage particles isolated from fish (Mediterranean, Atlantic, farmed, and frozen), the most abundant being β-lactamases. Some of these particles were infectious in cultures of the fecal bacteria Escherichia coli. By serving as ARG reservoirs in marine environments, including those with low human activity, such as the Antarctic, phages could contribute to ARG transmission between bacterial communities.

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“…Nevertheless, acquired antibiotic resistance is not exclusively triggered by the use of antibiotics. Bacterial resistance to heavy metals (e.g., copper, chromium, cadmium, arsenic) and antibiotics simultaneously increased with the presence of heavy metals alone ( Pruden et al, 2006 ; Knapp et al, 2011 ; Tan et al, 2018 ); hence, water pollution can also be a source of antibiotic resistance development in V. anguillarum . For example, the tet(34) gene increased by 27.70 times in bacteria exposed to arsenic for six hours, and the co-selected resistance was well-maintained for up to seven days without selective pressure ( Zhang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of 44 Vibrio anguillarum genomes reveals high genetic diversity

Hansen

Kudirkienė

Dalsgaard

2020

PeerJ

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Vibriosis, a hemorrhagic septicemic disease caused by the bacterium Vibrio anguillarum, is an important bacterial infection in Danish sea-reared rainbow trout. Despite of vaccination, outbreaks still occur, likely because the vaccine is based on V. anguillarum strains from abroad/other hosts than rainbow trout. Information about the genetic diversity of V. anguillarum specifically in Danish rainbow trout, is required to investigate this claim. Consequently, the aim of the present investigation was to sequence and to characterize a collection of 44 V. anguillarum strains obtained primarily from vibriosis outbreaks in Danish rainbow trout. The strains were sequenced, de novo assembled, and the genomes examined for the presence of plasmids, virulence, and acquired antibiotic resistance genes. To investigate the phylogeny, single nucleotide polymorphisms were identified, and the pan-genome was calculated. All strains carried tet(34) encoding tetracycline resistance, and 36 strains also contained qnrVC6 for increased fluoroquinolone/quinolone resistance. But interestingly, all strains were phenotypic sensitive to both oxytetracycline and oxolinic acid. Almost all serotype O1 strains contained a pJM1-like plasmid and nine serotype O2A strains carried the plasmid p15. The distribution of virulence genes was rather similar across the strains, although evident variance among serotypes was observed. Most significant, almost all serotype O2 and O3 strains, as well as the serotype O1 strain without a pJM1-like plasmid, carried genes encoding piscibactin biosynthesis. Hence supporting the hypothesis, that piscibactin plays a crucial role in virulence for pathogenic strains lacking the anguibactin system. The phylogenetic analysis and pan-genome calculations revealed great diversity within V. anguillarum. Serotype O1 strains were in general very similar, whereas considerable variation was found among serotype O2A strains. The great diversity within the V. anguillarum serotype O2A genomes is most likely the reason why vaccines provide good protection from some strains, but not from others. Hopefully, the new genomic data and knowledge provided in this study might help develop an optimized vaccine against V. anguillarum in the future to reduce the use of antibiotics, minimize economic losses and improve the welfare of the fish.

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Arctic antibiotic resistance gene contamination, a result of anthropogenic activities and natural origin

Cited by 103 publications

References 82 publications

Bacterial Heavy-Metal and Antibiotic Resistance Genes in a Copper Tailing Dam Area in Northern China

Bacterial Heavy-Metal and Antibiotic Resistance Genes in a Copper Tailing Dam Area in Northern China

Antibiotic Resistance Genes in Phage Particles from Antarctic and Mediterranean Seawater Ecosystems

Analysis of 44 Vibrio anguillarum genomes reveals high genetic diversity

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