Arcobacter thereius sp. nov., isolated from pigs and ducks

Houf, Kurt; On, Stephen L. W.; Coenye, Tom; Debruyne, Lies; Smet, Sarah De; Vandamme, Peter

doi:10.1099/ijs.0.006650-0

Cited by 78 publications

(59 citation statements)

References 37 publications

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“…On et al (119)(120)(121) standardized the inoculum and biochemical identification tests for campylobacters, and from their results a set of tests useful for distinguishing Arcobacter species were proposed in Bergey's Manual of Systematic Bacteriology in 2005 (148). However, eight new Arcobacter species have been described since then (24,28,34,35,52,74,75,94). Table 5 shows the most useful biochemical tests for differentiating the currently accepted and recently proposed Arcobacter species.…”

Section: Identification Phenotypic Identificationmentioning

confidence: 99%

“…Very recently, six new species have been added to the genus, which now therefore includes 12 species. Arcobacter mytili, isolated from mussels and brackish water in Spain, was the first species of the genus that is unable to hydrolyze indoxyl acetate (24); Arcobacter thereius has been isolated from livers and kidneys of spontaneously aborted porcines and from duck cloacal samples (74); Arcobacter marinus (reported on the basis of only one strain) has been isolated from a mixed sample of seawater, starfish, and seaweeds in Korea (94); Arcobacter trophiarum was isolated from feces of fattening pigs in Belgium (34); Arcobacter defluvii was isolated from sewage samples (28); and Arcobacter molluscorum was recovered from mussels and oysters and is the second species of the genus that does not hydrolyze indoxyl acetate (52). One strain isolated from a chicken cloacal swab sample in Valdivia (Chile) showed 99.9% 16S rRNA gene similarity (GenBank accession number GU300768) with the sequence of the type strain of A. trophiarum (34), indicating that the strain, recovered from a different origin and region, belonged to this new species (M. J. Figueras, L. Collado, A. Levican, and H. Fernández, unpublished data).…”

Section: Taxonomymentioning

confidence: 99%

“…Each of these methods has advantages and disadvantages related to its reproducibility, simplicity, discriminatory power, and cost (71). The most commonly used typing technique has been ERIC-PCR, which has been successfully applied for the investigation of outbreaks (150), for the characterization of isolates from foods and water (12,27,71), and for isolates included in the description of new Arcobacter species in order to find out whether they have a clonal origin (24,28,34,74,75). However, in the recent description of the new species A. trophiarum, 10 different AFLP profiles were recognized among the 16 isolates recovered from this species, while with ERIC-PCR only 4 genotypes could be identified (34).…”

Section: Genotypingmentioning

confidence: 99%

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Taxonomy, Epidemiology, and Clinical Relevance of the Genus Arcobacter

2011

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SUMMARY The genus Arcobacter , defined almost 20 years ago from members of the genus Campylobacter , has become increasingly important because its members are being considered emergent enteropathogens and/or potential zoonotic agents. Over recent years information that is relevant for microbiologists, especially those working in the medical and veterinary fields and in the food safety sector, has accumulated. Recently, the genus has been enlarged with several new species. The complete genomes of Arcobacter butzleri and Arcobacter nitrofigilis are available, with the former revealing diverse pathways characteristic of free-living microbes and virulence genes homologous to those of Campylobacter. The first multilocus sequence typing analysis showed a great diversity of sequence types, with no association with specific hosts or geographical regions. Advances in detection and identification techniques, mostly based on molecular methods, have been made. These microbes have been associated with water outbreaks and with indicators of fecal pollution, with food products and water as the suspected routes of transmission. This review updates this knowledge and provides the most recent data on the taxonomy, species diversity, methods of detection, and identification of these microbes as well as on their virulence potential and implication in human and animal diseases.

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Section: Identification Phenotypic Identificationmentioning

confidence: 99%

Section: Taxonomymentioning

confidence: 99%

Section: Genotypingmentioning

confidence: 99%

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Taxonomy, Epidemiology, and Clinical Relevance of the Genus Arcobacter

2011

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“…In humans, A. butzleri is predominantly associated with enteritis and septicemia (24,30,46), though Arcobacter cryaerophilus and Arcobacter skirrowii have also been isolated from diarrheal stool specimens (21,33,44). The other three species, Arcobacter cibarius (15), Arcobacter thereius (14), and Arcobacter trophiarum (4) are present in farm animals and on food of animal origin but have not yet been isolated from human specimens.…”

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confidence: 99%

Occurrence of Putative Virulence Genes in Arcobacter Species Isolated from Humans and Animals

et al. 2012

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Interest in arcobacters in veterinary and human public health has increased since the first report of the isolation of arcobacters from food of animal origin. Since then, studies worldwide have reported the occurrence of arcobacters on food and in food production animals and have highlighted possible transmission, especially of Arcobacter butzleri, to the human population. In humans, arcobacters are associated with enteritis and septicemia. To assess their clinical relevance for humans and animals, evaluation of potential virulence factors is required. However, up to now, little has been known about the mechanisms of pathogenicity. Because of their close phylogenetic affiliation to the food-borne pathogen Campylobacter and their similar clinical manifestations, the presence of nine putative Campylobacter virulence genes (cadF, ciaB, cj1349, hecA, hecB, irgA, mviN, pldA, and tlyA) previously identified in the recent Arcobacter butzleri ATCC 49616 genome sequence was determined in a large set of human and animal Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii strains after the development of rapid and accurate PCR assays and confirmed by sequencing and dot blot hybridization.A rcobacters are increasingly being isolated from a wide range of food products all over the world. These Gram-negative bacteria have been classified into the family Campylobacteraceae (35), although a recent annotation of the Arcobacter butzleri genome suggests a closer phylogenetic relation to Sulfurimonas denitrificans and Wolinella succinogenes, both members of the Helicobacteraceae, as well as to the deep-sea vent Epsilonproteobacteria members Sulfurovum and Nitratiruptor (26). At present, 13 Arcobacter species have been characterized, of which 6 were isolated from mammals. In humans, A. butzleri is predominantly associated with enteritis and septicemia (24,30,46), though Arcobacter cryaerophilus and Arcobacter skirrowii have also been isolated from diarrheal stool specimens (21,33,44). The other three species, Arcobacter cibarius (15), Arcobacter thereius (14), and Arcobacter trophiarum (4) are present in farm animals and on food of animal origin but have not yet been isolated from human specimens.Contaminated drinking water is identified as a major source of human Arcobacter infection in developing regions (1), whereas in industrialized countries, infections are assumed to be food-borne. Close contact with pets and person-to-person transmission are the other potential risk factors (9, 36). Arcobacters seem to be commonly present on food of animal origin, with the highest prevalence reported for poultry followed by pork and beef (38,42). The origin of the contamination on poultry products is still debated (39), but for pork and beef, feces transmitted during the slaughter process is regarded as the initial source of contamination (40, 44).The A. butzleri ATCC 49616 genome revealed that this strain has putative virulence determinants such as genes cadF and cj1349, coding for fibronectin binding proteins; the invasi...

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“…Arcobacter nitrofigilis and Arcobacter cryaerophilus were previously included in the genus Campylobacter and later reassigned to the genus Arcobacter. In the past decade, the genus Arcobacter has been expanded to include 14 species with the following chronological order of discovery: Arcobacter butzleri, Arcobacter skirrowii, Arcobacter cibarius, Arcobacter halophilus, Arcobacter mytili, Arcobacter thereius, Arcobacter marinus, Arcobacter trophiarum, Arcobacter defluvii, Arcobacter molluscorum, Arcobacter bivalviorum, and Arcobacter venerupis (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). Among the Arcobacter species described to date, A. butzleri, A. skirrowii, and A. cryaerophilus are considered human pathogens causing gastroenteritis or bacteremia (16).…”

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confidence: 99%

Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species

et al. 2014

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This study aimed to develop a loop-mediated isothermal amplification (LAMP) method for the rapid detection of Arcobacter species. Specific primers targeting the 23S ribosomal RNA gene were used to detect Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii. The specificity of the LAMP primer set was assessed using DNA samples from a panel of Arcobacter and Campylobacter species, and the sensitivity was determined using serial dilutions of Arcobacter species cultures. LAMP showed a 10-to 1,000-fold-higher sensitivity than multiplex PCR, with a detection limit of 2 to 20 CFU per reaction in vitro. Whereas multiplex PCR showed cross-reactivity with Campylobacter species, the LAMP method developed in this study was more sensitive and reliable than conventional PCR or multiplex PCR for the detection of Arcobacter species.

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Arcobacter thereius sp. nov., isolated from pigs and ducks

Cited by 78 publications

References 37 publications

Taxonomy, Epidemiology, and Clinical Relevance of the Genus Arcobacter

Taxonomy, Epidemiology, and Clinical Relevance of the Genus Arcobacter

Occurrence of Putative Virulence Genes in Arcobacter Species Isolated from Humans and Animals

Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species

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