Arcobacter butzleri survives within trophozoite of Acanthamoeba castellanii

Villanueva, María Paz; Medina, Gustavo; Fernández, Heriberto

doi:10.1016/j.ram.2015.12.003

Cited by 16 publications

(14 citation statements)

References 13 publications

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“…A. butzleri strains had the ability to survive inside the amoebae known as Acanthamoeba castellanii for the period of 240 h, which suggest that A. butzleri was resistant to amoebic digestion. Moreover, A. castellanii could act as an environmental reservoir and transmission vehicle for Arcobacter (Villanueva et al 2016).…”

Section: Epidemiology and Transmission In Animalsmentioning

confidence: 99%

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Ramees

Dhama

Karthik

et al. 2017

Veterinary Quarterly

127

107

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Arcobacter has emerged as an important food-borne zoonotic pathogen, causing sometimes serious infections in humans and animals. Newer species of Arcobacter are being incessantly emerging (presently 25 species have been identified) with novel information on the evolutionary mechanisms and genetic diversity among different Arcobacter species. These have been reported from chickens, domestic animals (cattle, pigs, sheep, horses, dogs), reptiles (lizards, snakes and chelonians), meat (poultry, pork, goat, lamb, beef, rabbit), vegetables and from humans in different countries. Arcobacters are implicated as causative agents of diarrhea, mastitis and abortion in animals, while causing bacteremia, endocarditis, peritonitis, gastroenteritis and diarrhea in humans. Three species including A. butzleri, A. cryaerophilus and A. skirrowii are predominantly associated with clinical conditions. Arcobacters are primarily transmitted through contaminated food and water sources. Identification of Arcobacter by biochemical tests is difficult and isolation remains the gold standard method. Current diagnostic advances have provided various molecular methods for efficient detection and differentiation of the Arcobacters at genus and species level. To overcome the emerging antibiotic resistance problem there is an essential need to explore the potential of novel and alternative therapies. Strengthening of the diagnostic aspects is also suggested as in most cases Arcobacters goes unnoticed and hence the exact epidemiological status remains uncertain. This review updates the current knowledge and many aspects of this important food-borne pathogen, namely etiology, evolution and emergence, genetic diversity, epidemiology, the disease in animals and humans, public health concerns, and advances in its diagnosis, prevention and control.

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Section: Epidemiology and Transmission In Animalsmentioning

confidence: 99%

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Ramees

Dhama

Karthik

et al. 2017

Veterinary Quarterly

127

107

View full text Add to dashboard Cite

show abstract

“…Arcobacter butzleri can establish a long-lasting relation with Acanthamoeba castellanii for up to 10 days in laboratory co-culture experiments [132]. The pathogen is also able to replicate inside the amoeba suggesting a possible endocytobiotic interaction and a possible role of the FLA as a transport vehicle for the bacteria [76,77].…”

Section: Host-microorganism Interactionsmentioning

confidence: 99%

Free-Living Amoebae as Hosts for and Vectors of Intracellular Microorganisms with Public Health Significance

Balczun

Scheid

2017

Viruses

116

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Free-living amoebae (FLA) are parasites within both humans and animals causing a wide range of symptoms and act as hosts of, and vehicles for phylogenetically diverse microorganisms, called endocytobionts. The interaction of the FLA with sympatric microorganisms leads to an exceptional diversity within FLA. Some of these bacteria, viruses, and even eukaryotes, can live and replicate intracellularly within the FLA. This relationship provides protection to the microorganisms from external interventions and a dispersal mechanism across various habitats. Among those intracellularly-replicating or -residing organisms there are obligate and facultative pathogenic microorganisms affecting the health of humans or animals and are therefore of interest to Public Health Authorities. Mimiviruses, Pandoraviruses, and Pithoviruses are examples for interesting viral endocytobionts within FLA. Future research is expected to reveal further endocytobionts within free-living amoebae and other protozoa through co-cultivation studies, genomic, transcriptomic, and proteomic analyses.

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“…It has been previously shown that FLA can act as hosts for some bacterial pathogens which are able to resist amoebal digestion (amoeba resisting bacteria, ARB). Different pathogens such as Legionella, Mycobacterium, Aeromonas, Listeria, Arcobacter or Campylobacter have been isolated from inside FLA in co-culture assays (Steinert et al, 1998;García et al, 2007;Snelling et al, 2008;Akya et al, 2009;Bui et al, 2012;Villanueva et al, 2016). Inside them, these bacteria survive and are more resistant to harsh environmental conditions that would normally kill them, such as chlorination or presence of biocides (Thomas et al, 2010;Dupuy et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Detection of viable Helicobacter pylori inside free‐living amoebae in wastewater and drinking water samples from Eastern Spain

Moreno-Mesonero

Moreno

Alonso

et al. 2017

Environmental Microbiology

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Helicobacter pylori is one of the most concerning emerging waterborne pathogens. It has been suggested that it could survive in water inside free-living amoebae (FLA), but nobody has studied this relationship in the environment yet. Thus, we aimed to detect viable H. pylori cells from inside FLA in water samples. Sixty-nine wastewater and 31 drinking water samples were collected. FLA were purified and identified by PCR and sequencing. For exclusively detecting H. pylori inside FLA, samples were exposed to sodium hypochlorite and assayed by specific PMA-qPCR, DVC-FISH and culture. FLA were detected in 38.7% of drinking water and 79.7% of wastewater samples, even after disinfection. In wastewater, Acanthamoeba spp. and members of the family Vahlkampfiidae were identified. In drinking water, Acanthamoeba spp. and Echinamoeba and/or Vermamoeba were present. In 39 (58.2%) FLA-positive samples, H. pylori was detected by PMA-qPCR. After DVC-FISH, 21 (31.3%) samples harboured viable H. pylori internalized cells. H. pylori was cultured from 10 wastewater samples. To our knowledge, this is the first report that demonstrates that H. pylori can survive inside FLA in drinking water and wastewater, strongly supporting the hypothesis that FLA could play an important role in the transmission of H. pylori to humans.

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Arcobacter butzleri survives within trophozoite of Acanthamoeba castellanii

Cited by 16 publications

References 13 publications

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Free-Living Amoebae as Hosts for and Vectors of Intracellular Microorganisms with Public Health Significance

Detection of viable Helicobacter pylori inside free‐living amoebae in wastewater and drinking water samples from Eastern Spain

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