Archiving genetically altered animals: a review of cryopreservation and recovery methods for genome edited animals

Hart-Johnson, Sarah; Mankelow, Katharine

doi:10.1177/00236772211007306

Cited by 12 publications

(10 citation statements)

References 79 publications

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“…The new era of genome editing has accelerated the generation of GEMMs reinforcing the need for management of their numbers. Sperm cryopreservation represents an efficient and economic method for archiving and distribution of mice [ 2 ]. C57BL/6 is the most common background for GEMMs but sperm cryopreservation has long been challenging for this strain [ 4 – 7 ].…”

Section: Discussionmentioning

confidence: 99%

“…To meet the demand for efficient and cost-effective archiving, distribution and colony management of these strains, cryopreservation of sperm for subsequent rederivation via i n v itro f ertilization (IVF) has become the method of choice. In contrast to embryos the cryopreservation of sperm does not involve breeding efforts, requires far less animals and enables generation of a large number of embryos upon rederivation [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

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A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm

2021

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The advent of genome editing tools like CRISPR/Cas has substantially increased the number of genetically engineered mouse models in recent years. In support of refinement and reduction, sperm cryopreservation is advantageous compared to embryo freezing for archiving and distribution of such mouse models. The in vitro fertilization using cryopreserved sperm from the most widely used C57BL/6 strain has become highly efficient in recent years due to several improvements of the procedure. However, purchase of the necessary media for routine application of the current protocol poses a constant burden on budgetary constraints. In-house media preparation, instead, is complex and requires quality control of each batch. Here, we describe a cost-effective and easily adaptable approach for in vitro fertilization using cryopreserved C57BL/6 sperm. This is mainly achieved by modification of an affordable commercial fertilization medium and a step-by-step description of all other necessary reagents. Large-scale comparison of fertilization rates from independent lines of genetically engineered C57BL/6 mice upon cryopreservation and in vitro fertilization with our approach demonstrated equal or significantly superior fertilization rates to current protocols. Our novel SEcuRe (Simple Economical set-up for Rederivation) method provides an affordable, easily adaptable and harmonized protocol for highly efficient rederivation using cryopreserved C57BL/6 sperm for a broad application of colony management in the sense of the 3Rs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm

2021

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“…Moreover, Seki and Rutz have optimized the method for Cas9/RNP (Ribonucleoprotein) transfection of primary mouse and human T cells without T cell receptor (TCR) stimulation, which almost causes the entire inhibition of target gene expression. This finding significantly simplify the gene editing process for next-generation immunotherapies [36].CRISPR/Cas systems are also capable of modifying genome in other experimental models including mouse and zebrafish [37].…”

Section: Genome Editingmentioning

confidence: 97%

Recent advances of the biological and biomedical applications of CRISPR/Cas systems

Wang

Zhao

2022

Mol Biol Rep

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It has also been reported that about 40% of spacers from lactic acid bacteria, namely Streptococcus thermophiles, have a homologue corresponding to either phage or plasmid DNA sequences among the isolated and sequenced phage's genome [3]. Thus, the spacers are proposed to be derived from foreign DNA including phage and plasmids. Bacteria generally acquire new spacers once they are exposed to new viruses in order to confer resistance to the cognate virus. Therefore, spacers present in a certain bacterium typically reveal the historical record of phages that the bacterium has been exposed to [4]. The leader sequence is commonly located 5' to the CRISPR locus and is rich in AT nucleotides. However, it has been demonstrated that the leader sequence is incapable of encoding RNAs or proteins, and that it is located next to the short repeats. Apart from the leader sequence, the genes encoding Cas proteins are found to be localized either upstream or downstream of the CRISPR locus.The CRISPR/Cas system is postulated to be formed in three stages. In the adaption stage, bacterial cells acquire sequences derived from foreign DNA such as viral DNA, and then incorporate them into the bacterial CRISPR array. During the expression stage, precursor CRISPR RNA (crRNA)

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“…The new era of genome editing has accelerated the generation of GEMMs reinforcing the need for management of their numbers. Sperm cryopreservation represents an efficient and economic method for archiving and distribution of mice [2]. C57BL/6 is the most common background for GEMMs but sperm cryopreservation has long been challenging for this strain [4][5][6][7].…”

Section: Discussionmentioning

confidence: 99%

“…To meet the demand for efficient and cost-effective archiving, distribution and colony management of these strains, cryopreservation of sperm for subsequent rederivation via in vitro fertilization (IVF) has become the method of choice. In contrast to embryos the cryopreservation of sperm does not involve breeding efforts, requires far less animals and enables generation of a large number of embryos upon rederivation [2].…”

Section: Introductionmentioning

confidence: 99%

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Archiving genetically altered animals: a review of cryopreservation and recovery methods for genome edited animals

Cited by 12 publications

References 79 publications

A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm

A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm

Recent advances of the biological and biomedical applications of CRISPR/Cas systems

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