Architecture of the streptomyces lividans DnaA protein-replication origin complexes

Sequence Recognition, Cooperative Interaction, and Dimerization of the Initiator Protein DnaA of Streptomyces

“…According to our previous studies, the formation of the Streptomyces initial nucleoprotein complex involves the sequential binding of the DnaA protein molecules to 19 DnaA boxes. Subsequent protein-protein interaction leads to bending and looping of the Streptomyces oriC region (18).…”

“…The Streptomyces DnaA is the first chromosomal initiator for which the kinetics of two dimerization domains, I and III, have been determined. These domains dimerize independently (18). The dimerization of the DnaA protein does not occur in the absence of DNA.…”

“…Recently, we have shown that binding of the DnaA protein to both DnaA boxes exhibits a cooperative character (16). Domains I and III independently participate in the dimerization of the DnaA protein molecules (18). To evaluate in detail the kinetics of binding of the DnaA protein to DnaA boxes from the dnaA gene promoter region, we applied gel mobility shift assays to determine the binding constants for cooperativity as well as for dimerization (20).…”

“…Recently, it has been shown that the domains I and III are independently involved in dimerization of the Streptomyces lividans DnaA protein molecules. The interaction of Streptomyces DnaA protein with two DnaA boxes is cooperative and accompanied by strong DNA bending (16,18). However, we do not know the contribution of the different domains of the Streptomyces DnaA protein to cooperative binding.…”

Soil To Genomics: The Streptomyces Chromosome

“…However, whereas these other genomes initiate replication from the TP as a single strand from either end of the molecule (100), Streptomyces chromosomes replicate bidirectionally from a typical, centrally placed oriC (65). The two replication forks of circular bacterial chromosomes meet to complete the daughter molecule, but linear replicons face an "end problem": the leading strand is synthesized right to the chromosome ends, but removal of the RNA primer for the last Okazaki fragment of the lagging strand leaves a single-stranded 3 -gap, which is "end-patched" by DNA synthesis primed from the TP [the gap was estimated as ∼280 nt for pSLA2(21) and for some other Streptomyces replicons (C-H. Huang & C. W. Chen, personal communication)].…”