Architecture of the chloroplast PSI–NDH supercomplex in Hordeum vulgare

Shen, Liangliang; Tang, Kailu; Wang, Wenda; Wang, Chen; Wu, Haixiao; Mao, Zhiyuan; An, Shaoya; Chang, Shenghai; Kuang, Tingyun; Shen, Jian‐Ren; Han, Guangye; Zhang, Xing

doi:10.1038/s41586-021-04277-6

Cited by 42 publications

(46 citation statements)

References 51 publications

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“…Further focused refinement (Zhu et al, 2018) on two PSIs and two NDH regions (SubM-SubB-SubL and SubA-SubE) improved the resolution of these individual parts to 3.3 A ˚, 3.3 A ˚, 3.6 A ˚, and 4.3 A ˚, respectively (Supplemental Figure 3). The structure shows that one NDH complex is sandwiched between two PSIs (Figure 1), consistent with early negative staining results (Kouril et al, 2014;Yadav et al, 2017) and with the HvNDH-PSI structure (Shen et al, 2021). A total of 58 protein subunits and 415 cofactors were identified in the supercomplex structure (Supplemental Table 2).…”

Section: Overall Structuresupporting

confidence: 81%

“…They have been suggested to localize in between the left PSI and NDH, or each replaces one major Lhca in the LHCI belt of each PSI, mediating the interactions of PSI-LHCI with NDH (Kouril et al, 2014;Otani et al, 2018). Recently, a 4.4 A ˚resolution structure of the NDH-PSI 2 supercomplex from Hordeum vulgare (HvNDH-PSI) was reported (Shen et al, 2021). These previously reported biochemical and structural data have provided an important basis for understanding the subunit arrangement of NDH and its interaction with PSI.…”

Section: Introductionmentioning

confidence: 99%

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Supramolecular assembly of chloroplast NADH dehydrogenase-like complex with photosystem I from Arabidopsis thaliana

et al. 2022

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Section: Overall Structuresupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Supramolecular assembly of chloroplast NADH dehydrogenase-like complex with photosystem I from Arabidopsis thaliana

et al. 2022

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“…According to the molecular mass of PSI (~ 580 kDa) and NDH (~ 550 kDa), the PSI-mc1 and PSI-mc2 may represent PSI/NDH ratio of two and one, respectively. PSI megacomplexes with different PSI/NDH ratio of two and more were also detected earlier [ 34 , 37 , 43 , 48 , 49 , 70 , 71 ]. In agreement with above mentioned data, the relationship of PSI-mc1,2,4 bands obtained by the second-dimensional BN PAGE verified that the solubilisation products of PSI-mc1 were PSI-mc1,2,4, and NDH complex and those of PSI-mc2 PSI-mc2,4 and NDH complex (Fig.…”

Section: Resultssupporting

confidence: 65%

Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE

et al. 2022

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Background Blue Native polyacrylamide gel electrophoresis (BN PAGE) followed by denaturing PAGE is a widely used, convenient and time efficient method to separate thylakoid complexes and study their composition, abundance, and interactions. Previous analyses unravelled multiple monomeric and dimeric/oligomeric thylakoid complexes but, in certain cases, the separation of complexes was not proper. Particularly, the resolution of super- and megacomplexes, which provides important information on functional interactions, still remained challenging. Results Using a detergent mixture of 1% (w/V) n-dodecyl-β-d-maltoside plus 1% (w/V) digitonin for solubilisation and 4.3–8% gel gradients for separation as methodological improvements in BN PAGE, several large photosystem (PS) I containing bands were detected. According to BN(/BN)/SDS PAGE and mass spectrometry analyses, these PSI bands proved to be PSI-NADH dehydrogenase-like megacomplexes more discernible in maize bundle sheath thylakoids, and PSI complexes with different light-harvesting complex (LHC) complements (PSI-LHCII, PSI-LHCII*) more abundant in mesophyll thylakoids of lincomycin treated maize. For quantitative determination of the complexes and their comparison across taxa and physiological conditions, sample volumes applicable to the gel, correct baseline determination of the densitograms, evaluation methods to resolve complexes running together, calculation of their absolute/relative amounts and distribution among their different forms are proposed. Conclusions Here we report our experience in Blue/Clear-Native polyacrylamide gel electrophoretic separation of thylakoid complexes, their identification, quantitative determination and comparison in different samples. The applied conditions represent a powerful methodology for the analysis of thylakoid mega- and supercomplexes.

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“…This allows eukaryotic cells to adapt metabolically to nutrient and proteome limitations. Crowding of large complexes in the membrane systems of thylakoid 35, 36 , mitochondrial cristae 37 , as well as in the cytosol, e.g. ribosome dimers 38 is an established and conserved mechanism for a more efficient differentiation of functions, ecological adaptation, and material storage during less active periods.…”

Section: Mainmentioning

confidence: 99%

Algal photosystem I dimer and high resolution model of PSI:plastocyanin complex

Naschberger

Mosebach

Tobiasson

et al. 2021

Preprint

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Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular organization affects the dynamics of photosynthetic membranes. Here, we reveal a chloroplast PSI from the green alga Chlamydomonas reinhardtii that is organized as a homodimer, comprising 40 protein subunits with 118 transmembrane helices that provide scaffold for 568 pigments. Our cryo-EM structure identifies the light-harvesting protein Lhca9 as the key element for the dimerization. Furthermore, the absence of Lhca2 and PsaH, gives rise to a head-to-head relative orientation of the PSI-LHCI monomers, in a way that is essentially different from the oligomer formation in cyanobacteria. The interface between the monomers partially overlaps with the surface area that would bind one of the LHCII complexes in state transitions. We also define the most accurate available PSI-LHCI model at 2.3 Å resolution, including a flexibly bound electron donor plastocyanin, and assign correct identities and orientations of all the pigments, as well as 486 water molecules that affect energy transfer pathways.

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Architecture of the chloroplast PSI–NDH supercomplex in Hordeum vulgare

Cited by 42 publications

References 51 publications

Supramolecular assembly of chloroplast NADH dehydrogenase-like complex with photosystem I from Arabidopsis thaliana

Supramolecular assembly of chloroplast NADH dehydrogenase-like complex with photosystem I from Arabidopsis thaliana

Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE

Algal photosystem I dimer and high resolution model of PSI:plastocyanin complex

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