Architecture of a dual biocompatible platform to immobilize genistin: Fabrication with physio-chemical and in vitro evaluation

Abstract

In this research, genistin was obtained by extracting from soybean meal powder, and then by adding polycaprolactone (PCL), genistin nanocapsules were prepared. For the first time, we used a lipophilic nanophase (encapsulated genistin) coated in hydrophilic nanofibers (gelatin /polyvinyl alcohol) as a dual nanosystem by the electrospinning method. In the approach, the nanofibers mimic the natural extracellular matrix, interact favorably with cells being cultured from one side, and raise the local concentration of a bioactive compound at the cell surface. The encapsulated drug was loaded in fibers with various percentages, which showed appropriate and significant controlled release using high-performance liquid chromatography (HPLC). To prove the experiments, analysis using an ultraviolet-visible spectrometer (UV-Vis), 1 H NMR spectrometer, Fourier transforms infrared spectrometer (FTIR), mechanical test, scanning electron microscope (SEM) and microscope transmission electron microscopy (TEM) was performed. Finally, excellent biological effects, viability, and non-toxicity of the sample synthesized by the MTT method and significant proliferation and adhesion on mouse fibroblast cell line L929 were obtained.