Architectural Transcription Factor HMGI(Y) Promotes Tumor Progression and Mesenchymal Transition of Human Epithelial Cells

Identification of molecular markers and signaling pathway in endometrial cancer in Hong Kong Chinese women by genome-wide gene expression profiling

“…CCNA1 is the cyclin required for progression from S phase into G2, whereas CCNE1 is necessary for transition through the G1/S and G2/M checkpoints (Schafer, 1998). Increased CCN1A expression may be stimulated by the transcription factors MYB and HMGA1 (Muller et al, 1999;Reeves et al, 2001). In addition, downregulation of WT-1 is associated with increased expression of cyclin E1 (Loeb et al, 2002).…”

Gene-specific nucleotide excision repair is impaired in human cells expressing elevated levels of high mobility group A1 nonhistone proteins

“…HTB-22), were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine, 10mM HEPES, 100 units/mL penicillin G sodium and 100μg/mL streptomycin sulfate. For routine maintenance, almost confluent monolayer cultures of cells were digested to single cell suspensions by gentle trypsinization, washed with phosphate buffered saline (PBS), counted and seeded into 100 mm culture dishes, as previously described [13]. Clonal cell lines MCF7-7C-Cs8 and MCF7-7C-Cs9 are stably transfected derivatives of MCF7/Tet-"OFF" cells containing a tetracycline-regulated pTRE vector encoding Hemagglutinin (HA)-tagged HMGA1a cDNA, maintained in the presence of 100 μg/ml of hygromycin [13].…”

“…For routine maintenance, almost confluent monolayer cultures of cells were digested to single cell suspensions by gentle trypsinization, washed with phosphate buffered saline (PBS), counted and seeded into 100 mm culture dishes, as previously described [13]. Clonal cell lines MCF7-7C-Cs8 and MCF7-7C-Cs9 are stably transfected derivatives of MCF7/Tet-"OFF" cells containing a tetracycline-regulated pTRE vector encoding Hemagglutinin (HA)-tagged HMGA1a cDNA, maintained in the presence of 100 μg/ml of hygromycin [13]. These different transgenic cell lines express high, but variable levels of transgenic HA-tagged HMGA1a protein when grown in medium lacking tetracycline (these are referred to as HMGA1 "ON" cells).…”

“…These genes were chosen because microarray and other analyses indicated that HMGA1 proteins do not regulate the transcription of either gene in the cell lines being investigated (unpublished data), an important consideration given that these proteins can either positively or negatively regulate the transcription of a large number of genes in vivo (reviewed in: [12]). The cell lines used in this study include stable transgenic MCF7 human breast cancer epithelial cell lines that contain a tetracycline-regulated HMGA1a transgene which can be experimentally induced (or not) to over-express HMGA1 protein, as previously described [13]. For comparison, we also assayed the efficiency of UV-induced lesion removal in non-transgenic MCF7 cells that express little, or no, detectable endogenous HMGA1 and in human embryonic fibroblasts (IMR90 cells), which naturally over-expresses high levels of these proteins.…”

AKNA: Another AT-hook transcription factor “hooking-up” with inflammation

“…The high mobility group A (HMGA) proteins are an important family of AT-hook chromatin remodeling proteins that orchestrate transcriptional complexes to regulate gene expression [1]. Recent studies have uncovered links between HMG AT-hook transcription factors and inflammation [1][2][3][4][5][6][7][8]. Moreover, emerging evidence also indicates that inflammatory pathways and downstream effects on tissues are important precursor lesions in diverse cancers [1].…”