Architectural Arrangement of Neurons as Part of the Functional Unit of the Central Nervous System

Orehek, Allen J.; Iglesias-Rozas, José R.; Garrosa, Manuel

doi:10.2139/ssrn.3403363

Cited by 1 publication

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“…S2). Layer IV, the internal granular layer with a denser nuclei distribution between Layer II and the WM [30], can be defined using H&E staining on the GE slide. PCP4 gene expression was visualized on the spatial map to show up Layer V [29].…”

Section: Annotation Of Cortical Layers and The Wm Of The Human Mtgmentioning

confidence: 99%

Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer’s disease

Chen

Chang

et al. 2022

acta neuropathol commun

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Human middle temporal gyrus (MTG) is a vulnerable brain region in early Alzheimer’s disease (AD), but little is known about the molecular mechanisms underlying this regional vulnerability. Here we utilize the 10 × Visium platform to define the spatial transcriptomic profile in both AD and control (CT) MTG. We identify unique marker genes for cortical layers and the white matter, and layer-specific differentially expressed genes (DEGs) in human AD compared to CT. Deconvolution of the Visium spots showcases the significant difference in particular cell types among cortical layers and the white matter. Gene co-expression analyses reveal eight gene modules, four of which have significantly altered co-expression patterns in the presence of AD pathology. The co-expression patterns of hub genes and enriched pathways in the presence of AD pathology indicate an important role of cell–cell-communications among microglia, oligodendrocytes, astrocytes, and neurons, which may contribute to the cellular and regional vulnerability in early AD. Using single-molecule fluorescent in situ hybridization, we validated the cell-type-specific expression of three novel DEGs (e.g., KIF5A, PAQR6, and SLC1A3) and eleven previously reported DEGs associated with AD pathology (i.e., amyloid beta plaques and intraneuronal neurofibrillary tangles or neuropil threads) at the single cell level. Our results may contribute to the understanding of the complex architecture and neuronal and glial response to AD pathology of this vulnerable brain region.

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Section: Annotation Of Cortical Layers and The Wm Of The Human Mtgmentioning

confidence: 99%

Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer’s disease

Chen

Chang

et al. 2022

acta neuropathol commun

View full text Add to dashboard Cite

show abstract

Architectural Arrangement of Neurons as Part of the Functional Unit of the Central Nervous System

Cited by 1 publication

References 0 publications

Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer’s disease

Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer’s disease

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