Archetypical Conductive Polymer Structure for Specific Interaction with Proteins

Reuven, Darkeyah G.; Sil, Dwaipayan; Sannigrahi, Biswajit; Wang, Xiaoqian; Baird, Barbara; Khan, Ishrat M.

doi:10.1080/10601325.2012.662073

Cited by 3 publications

(3 citation statements)

References 12 publications

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“…the approach reported holds promise for developing biomarker detection device. 6,7 The polymerization of the first monomer, 2-methoxystyrene, is 100% complete within 40 min i.e. 100% of the monomer is converted to the polymer and the second monomer polymerization is slow requiring 2 days to polymerize.…”

Section: Discussionmentioning

confidence: 99%

Procedure for Fabricating Biofunctional Nanofibers

Doss

Olubi

Sannigrahi

et al. 2012

JoVE

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Electrospinning is an effective processing method for preparing nanofibers decorated with functional groups. Nanofibers decorated with functional groups may be utilized to study material-biomarker interactions i.e. act as biosensors with potential as single molecule detectors. We have developed an effective approach for preparing functional polymers where the functionality has the capacity of specifically binding with a model protein. In our model system, the functional group is 2,4-dinitrophenyl (DNP) and the protein is anti-DNP IgE H NMR and Gel Permeation Chromatography (GPC). The molecular weight distributions of the polymers were narrow (1.1-1.2) and polymers with molecular weights greater than 50,000 was used in this study. The polymers were yellow powders and soluble in tetrahydrofuran. A water soluble CDNP-PEO-P2MS-PEO-CDNP/ DMEG (dimethoxyethylene glycol) complex binds and achieves steady state binding with solution IgE within a few seconds. Higher molecular weight (water insoluble i.e. around 50,000) CDNP-PEO-P2MS-PEO-CDNP polymers, containing 1% single wall carbon nanotubes (SWCNT) were processed into electroactive nanofibers (100 nm to 500 nm in diameter) on silicon substrate. Fluorescence spectroscopy shows that anti-DNP IgE interacts with the nanofibers by binding with the DNP functional groups decorating the fibers. These observations suggest that appropriately functionalized nanofibers hold promise for developing biomarker detection device. Video LinkThe video component of this article can be found at

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Section: Discussionmentioning

confidence: 99%

Procedure for Fabricating Biofunctional Nanofibers

Doss

Olubi

Sannigrahi

et al. 2012

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“…Poly(ethylene oxide) has added advantages of surface activity if it is part of block copolymer. Electroactive block of copolymers [22] or hybrid hydrogel [23,24] could introduce conductivity which could essentially develop variety of applications such as biosensors, electrostimulated drug release devices, etc. Cytocompatible poly(2-hydroxy ethyl methacrylate) as a part of block copolymer could enhance the attachment and growth of various dependent cells [25].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Bioactive Surfaces by Functionalization of Electroactive and Surface-Active Block Copolymers

et al. 2014

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Biofunctional block copolymers are becoming increasingly attractive materials as active components in biosensors and other nanoscale electronic devices. We have described two different classes of block copolymers with biofuctional properties. Biofunctionality for block copolymers is achieved through functionalization with appropriate biospecific ligands. We have synthesized block copolymers of electroactive poly(3-decylthiophene) and 2-hydroxyethyl methacrylate by atom transfer radical polymerization. The block copolymers were functionalized with the dinitrophenyl (DNP) groups, which are capable of binding to Immunoglobulin E (IgE) on cell surfaces. The block copolymers were shown to be redox active. Additionally, the triblock copolymer of α, ω-bi-biotin (poly(ethylene oxide)-b-poly (styrene)-b-poly(ethylene oxide)) was also synthesized to study their capacity to bind fluorescently tagged avidin. The surface-active property of the poly(ethylene oxide) block improved the availability of the biotin functional groups on the polymer surfaces. Fluorescence microscopy observations confirm the specific binding of biotin with avidin.

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“…α,ω‐2,4‐dinitrophenyl (DNP) groups, which serve as a useful model system . We have recently reported on the biofunctional properties of α,ω‐bi[2,4‐dinitrophenylcaproic][poly(ethyleneoxide)‐b‐poly(2‐methoxystyrene)‐b‐poly(ethylene oxide) (henceforth referred to as P2MS) and a poly(pyrrole)‐based DNP‐functionalized electroactive polymer . In a preliminary fashion, both these polymers were electrospun into nanofibers in order to determine processability such that the polymers may be fabricated into components to develop a nanoscale sensor for antibodies of defined specificity.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of electroactive composite nanofibers of functionalized polymer and CNT capable of specifically binding with the IgE (Immunoglobulin E) antibody

Olubi

Gadi

Sannigrahi

et al. 2013

Surface & Interface Analysis

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Electroactive nanofibers decorated with a,v-bi-(dinitrophenylcaproic acid), abbreviated as DNP, have been prepared by electrospinning a solution of a,v-bi [2,4-dinitrophenylcaproic][poly(ethyleneoxide)-b-poly(2-methoxystyrene)-b-poly (ethylene oxide)], polystyrene and single-walled carbon nanotubes (SWCNTs). The a,v-bi [2,4-dinitrophenylcaproic] [poly(ethyleneoxide)-b-poly(2-methoxystyrene)-b-poly(ethyleneoxide)] polymers were synthesized by living anionic polymerization and subsequent esterification to obtain the DNP-functionalized polymers. The nanofibers (300 nm) were electrospun onto a silicon wafer substrate at a voltage of 10 kV using dimethylformamide and chlorobenzene as solvents. The nanofibers were characterized by Raman spectroscopy, fluorescence microscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM) and optical microscopy. The DNP groups, therein, were tethered to the nanofibers via oligo(oxyethylene) spacers. The DNP groups decorating the nanofibers were capable of specifically binding with anti-DNP immunoglobulin E (IgE). The binding specificity of nanofibers containing 1% SWCNTs with anti-DNP IgE was studied via fluorescently labeled (Fluorescein IsoThioCyanate) FITC-IgE. Electronic activity of the nanofibers was studied by I-V plots from Kelvin sensing. The I-V plots before sensitizing with IgE differed from the I-V plots after binding with IgE. The results of the studies suggest the possibility of developing functional nanofibers as the active component in biosensors.

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Archetypical Conductive Polymer Structure for Specific Interaction with Proteins

Cited by 3 publications

References 12 publications

Procedure for Fabricating Biofunctional Nanofibers

Procedure for Fabricating Biofunctional Nanofibers

Fabrication of Bioactive Surfaces by Functionalization of Electroactive and Surface-Active Block Copolymers

Fabrication of electroactive composite nanofibers of functionalized polymer and CNT capable of specifically binding with the IgE (Immunoglobulin E) antibody

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