Archaeal TFEα/β is a hybrid of TFIIE and the RNA polymerase III subcomplex hRPC62/39

Blombach, Fabian; Salvadori, Enrico; Fouqueau, Thomas; Yan, Jun; Reimann, Julia; Sheppard, Carol; Smollett, Katherine; Albers, Sonja‐Verena; Kay, Christopher W. M.; Thalassinos, Konstantinos; Werner, Finn

doi:10.7554/elife.08378

Cited by 53 publications

(92 citation statements)

References 59 publications

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“…Like TFEα, TFEβ is also a bipartite protein consisting of an N-terminal classic winged-helix (WH) domain and a C-terminal domain containing a structural and seemingly redox inactive cubane [4Fe-4S] cluster. The former domain seems not to be required for TFE activity in vitro, while the latter domain is essential for heterodimerisation with the α−subunit and recruitment to the RNAP [11]. The gene encoding TFEβ is essential in Sulfolobus acidocaldarius [11].…”

Section: Topology Of the Archaeal Tata-tbp-tfb-rnap Pre-initiation Comentioning

confidence: 99%

“…Pioneering work from Wolfram Zillig's laboratory identified an AT-rich sequence upstream of the TSS important for the promoter activity [36]. Permanganate foot-printing experiments on the Sso and Mja OC have revealed similar boundaries for the initially melted region (IMR) of the promoter DNA extending up to position -12 relative to the TSS [11,37,38].…”

Section: A Recruitment Cascade Nucleates Transcription Initiationmentioning

confidence: 99%

“…Using chimeric archaeal promoter constructs we have shown that the IMR can change the promoter strength dramatically and independently of the TATA and BRE motifs of the promoter. The IMR is an important determinant for the strength of TFE stimulation [11]. However, it does not preclude additional sequencespecific interactions of this region with the transcription machinery, particularly RNAP, TFB and TFE.…”

Section: A Recruitment Cascade Nucleates Transcription Initiationmentioning

confidence: 99%

“…However, the prototypical TFEα/β exemplified by Sso is a heterodimer homologous to eukaryotic TFIIEα/β. In Sso the gene coding for TFEβ is essential while deletion of the gene coding for TFEα has not been attempted [11]. In the euryarchaeon Methanococcus maripaludis the gene coding for monomeric TFEα is essential [48].…”

Section: Topology Of the Archaeal Tata-tbp-tfb-rnap Pre-initiation Comentioning

confidence: 99%

“…The archaeal RNAP, RNAPII and -III employ a third conserved factor that stimulates OC formation, called transcription factor E (TFE), TFIIE and the RPC82/34 complex (hRPC62/39 in human), respectively [10]. Recent publications have provided intriguing insights into the structural organisation of these factors [11][12][13][14][15][16][17].…”

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confidence: 99%

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Molecular Mechanisms of Transcription Initiation—Structure, Function, and Evolution of TFE/TFIIE-Like Factors and Open Complex Formation

Blombach

Smollett

Grohmann

et al. 2016

Journal of Molecular Biology

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Open complex formation is an important target for the regulation of transcription in all domains of life. We propose that TFE and the bacterial general transcription factor CarD, though structurally and evolutionary unrelated, show interesting parallels in their mechanism to enhance open complex formation. We argue that open complex formation is used as a way 3 to regulate transcription in all domains of life, and these regulatory mechanisms co-evolved with the basal transcription machinery.

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Section: Topology Of the Archaeal Tata-tbp-tfb-rnap Pre-initiation Comentioning

confidence: 99%

Section: A Recruitment Cascade Nucleates Transcription Initiationmentioning

confidence: 99%

Section: A Recruitment Cascade Nucleates Transcription Initiationmentioning

confidence: 99%

Section: Topology Of the Archaeal Tata-tbp-tfb-rnap Pre-initiation Comentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular Mechanisms of Transcription Initiation—Structure, Function, and Evolution of TFE/TFIIE-Like Factors and Open Complex Formation

Blombach

Smollett

Grohmann

et al. 2016

Journal of Molecular Biology

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Structure and Function of RNA Polymerases and the Transcription Machineries

Griesenbeck

Tschochner

Grohmann

2017

Subcellular Biochemistry

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In all living organisms, the flow of genetic information is a two-step process: first DNA is transcribed into RNA, which is subsequently used as template for protein synthesis during translation. In bacteria, archaea and eukaryotes, transcription is carried out by multi-subunit RNA polymerases (RNAPs) sharing a conserved architecture of the RNAP core. RNAPs catalyse the highly accurate polymerisation of RNA from NTP building blocks, utilising DNA as template, being assisted by transcription factors during the initiation, elongation and termination phase of transcription. The complexity of this highly dynamic process is reflected in the intricate network of protein-protein and protein-nucleic acid interactions in transcription complexes and the substantial conformational changes of the RNAP as it progresses through the transcription cycle.In this chapter, we will first briefly describe the early work that led to the discovery of multisubunit RNAPs. We will then discuss the three-dimensional organisation of RNAPs from the bacterial, archaeal and eukaryotic domains of life, highlighting the conserved nature, but also the domain-specific features of the transcriptional apparatus. Another section will focus on transcription factors and their role in regulating the RNA polymerase throughout the different phases of the transcription cycle. This includes a discussion of the molecular mechanisms and dynamic events that govern transcription initiation, elongation and termination.

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A Global Characterisation of the Archaeal Transcription Machinery

Smollett

Blombach

Fouqueau

et al. 2017

RNA Metabolism and Gene Expression in Archaea

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Archaea employ a eukaryote-like transcription apparatus to transcribe a bacteria-like genome; while the RNA polymerase, basal factors and promoter elements mirror the eukaryotic RNA polymerase II system, archaeal genomes are densely packed with genes organised into multicistronic transcription units. The molecular mechanisms of archaeal transcription have been studied and characterised in great detail in vitro, but until recently relatively little was known about its global characteristics. In this chapter we discuss an integrated view of transcription from the molecular to the global level. Systems biology approaches have provided compelling insights into promoter and terminator DNA elements, the genome-wide distribution of transcription initiation-and elongation factors and RNA polymerase, the archaeal transcriptome and chromatin organisation. Overall these analyses illuminate transcription from a genome-wide perspective and serve as a resource for the community. In addition, Big Data can often validate mechanistic models based on biochemical and structural information, and generate new working hypotheses that can be thoroughly tested and dissected in vitro. This is an exciting time to study gene expression in the archaea since we are at the brink of a comprehensive yet detailed understanding of transcription.

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Archaeal TFEα/β is a hybrid of TFIIE and the RNA polymerase III subcomplex hRPC62/39

Cited by 53 publications

References 59 publications

Molecular Mechanisms of Transcription Initiation—Structure, Function, and Evolution of TFE/TFIIE-Like Factors and Open Complex Formation

Molecular Mechanisms of Transcription Initiation—Structure, Function, and Evolution of TFE/TFIIE-Like Factors and Open Complex Formation

Structure and Function of RNA Polymerases and the Transcription Machineries

A Global Characterisation of the Archaeal Transcription Machinery

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