Arbuscular mycorrhizal fungi play a role in protecting roots of Sophora viciifolia Hance. from Pb damage associated with increased phytochelatin synthase gene expression

Xu, Zhouying; Ban, Yihui; Li, Zhen; Chen, Hui; Yang, Ren; Tang, Ming

doi:10.1007/s11356-014-3209-9

Cited by 16 publications

(14 citation statements)

References 78 publications

(85 reference statements)

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“…[ 37 ]. Furthermore, AMF symbiosis also induce the expression of the phytochelatin synthase gene ( PCS1 ) in a leguminous plant ( Sophora viciifolia ) that was exposed to Pb pollution [ 42 ], indicating another mechanism for Pb immobilization in the roots of plants that were enhanced by AMF.…”

Section: Discussionmentioning

confidence: 99%

The Combined Effects of Arbuscular Mycorrhizal Fungi (AMF) and Lead (Pb) Stress on Pb Accumulation, Plant Growth Parameters, Photosynthesis, and Antioxidant Enzymes in Robinia pseudoacacia L.

et al. 2015

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Arbuscular mycorrhizal fungi (AMF) are considered as a potential biotechnological tool for improving phytostabilization efficiency and plant tolerance to heavy metal-contaminated soils. However, the mechanisms through which AMF help to alleviate metal toxicity in plants are still poorly understood. A greenhouse experiment was conducted to evaluate the effects of two AMF species (Funneliformis mosseae and Rhizophagus intraradices) on the growth, Pb accumulation, photosynthesis and antioxidant enzyme activities of a leguminous tree (Robinia pseudoacacia L.) at Pb addition levels of 0, 500, 1000 and 2000 mg kg-1 soil. AMF symbiosis decreased Pb concentrations in the leaves and promoted the accumulation of biomass as well as photosynthetic pigment contents. Mycorrhizal plants had higher gas exchange capacity, non-photochemistry efficiency, and photochemistry efficiency compared with non-mycorrhizal plants. The enzymatic activities of superoxide dismutase (SOD), ascorbate peroxidases (APX) and glutathione peroxidase (GPX) were enhanced, and hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents were reduced in mycorrhizal plants. These findings suggested that AMF symbiosis could protect plants by alleviating cellular oxidative damage in response to Pb stress. Furthermore, mycorrhizal dependency on plants increased with increasing Pb stress levels, indicating that AMF inoculation likely played a more important role in plant Pb tolerance in heavily contaminated soils. Overall, both F. mosseae and R. intraradices were able to maintain efficient symbiosis with R. pseudoacacia in Pb polluted soils. AMF symbiosis can improve photosynthesis and reactive oxygen species (ROS) scavenging capabilities and decrease Pb concentrations in leaves to alleviate Pb toxicity in R. pseudoacacia. Our results suggest that the application of the two AMF species associated with R. pseudoacacia could be a promising strategy for enhancing the phytostabilization efficiency of Pb contaminated soils.

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Section: Discussionmentioning

confidence: 99%

The Combined Effects of Arbuscular Mycorrhizal Fungi (AMF) and Lead (Pb) Stress on Pb Accumulation, Plant Growth Parameters, Photosynthesis, and Antioxidant Enzymes in Robinia pseudoacacia L.

et al. 2015

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“… 23 . In addition, the presence of AMF increased the expression of the phytochelatin synthase gene ( PCS1 ) in legume plant ( Sophora viciifolia ) exposed to Pb stress 24 , suggesting another mechanism of Pb immobilization in the roots of plants enhanced by mycorrhizal inoculation.…”

Section: Discussionmentioning

confidence: 99%

The roles of arbuscular mycorrhizal fungi (AMF) in phytoremediation and tree-herb interactions in Pb contaminated soil

Yang

Liang

Han

et al. 2016

Sci Rep

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105

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Understanding the roles of arbuscular mycorrhizal fungi (AMF) in plant interaction is essential for optimizing plant distribution to restore degraded ecosystems. This study investigated the effects of AMF and the presence of legume or grass herbs on phytoremediation with a legume tree, Robinia pseudoacacia, in Pb polluted soil. In monoculture, mycorrhizal dependency of legumes was higher than that of grass, and AMF benefited the plant biomass of legumes but had no effect on grass. Mycorrhizal colonization of plant was enhanced by legume neighbors but inhibited by grass neighbor in co-culture system. N, P, S and Mg concentrations of mycorrhizal legumes were larger than these of non-mycorrhizal legumes. Legume herbs decreased soil pH and thereby increased the Pb concentrations of plants. The neighbor effects of legumes shifted from negative to positive with increasing Pb stress levels, whereas grass provided a negative effect on the growth of legume tree. AMF enhanced the competition but equalized growth of legume-legume under unpolluted and Pb stress conditions, respectively. In conclusion, (1) AMF mediate plant interaction through directly influencing plant biomass, and/or indirectly influencing plant photosynthesis, macronutrient acquisition, (2) legume tree inoculated with AMF and co-planted with legume herbs provides an effective way for Pb phytoremediation.

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“…Standard and environmental DNA samples were added at 2.0 µL per reaction. The reaction was carried out on a CFX Connect Real-Time PCR Detection System (Bio-Rad, USA) using a program of 95 • C for 10 min, followed by 40 cycles of 95 • C for 30 s, 55 • C for 30 s and 72 • C for 30 s according to our previous study [42]. Specific amplification of the target gene was confirmed on a 1% (w/v) agarose gel showing the band of the expected size and melting curve analysis resulting in a single peak.…”

Section: Quantitative Pcrmentioning

confidence: 99%

Comparisons of Soil Properties, Enzyme Activities and Microbial Communities in Heavy Metal Contaminated Bulk and Rhizosphere Soils of Robinia pseudoacacia L. in the Northern Foot of Qinling Mountain

Yang

Dong

Cao

et al. 2017

Forests

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Abstract:The toxic effects of heavy metal (HM) contamination on plant metabolism and soil microorganisms have been emphasized recently; however, little is known about the differences in soil physical, chemical, and biological properties between bulk and rhizosphere soils contaminated with HMs in forest ecosystem. The present study was conducted to evaluate the rhizosphere effect on soil properties, enzyme activities and bacterial communities associated with Robinia pseudoacacia L. along a HM contamination gradient. Soil organic matter (SOM), available nitrogen (AN) and phosphorus (AP) contents were significantly higher in rhizosphere soil than those in bulk soil at HM contaminated sites (p < 0.05). Compared to bulk soil, activities of four soil enzymes indicative of C cycle (β-glucosidase), N cycle (protease, urease) and P cycle (alkaline phosphatase) in rhizosphere soil across all study sites increased by 47.5%, 64.1%, 52.9% and 103.8%, respectively. Quantitative PCR (qPCR) and restriction fragment length polymorphism (RFLP) were used to determine the relative abundance, composition and diversity of bacteria in both bulk and rhizosphere soils, respectively. The copy number of bacterial 16S rRNA gene in bulk soil was significantly lower than that in rhizosphere soil (p < 0.05), and it had significantly negative correlations with total/DTPA-extractable Pb concentrations (p < 0.01). Alphaproteobacteria, Gammaproteobacteria and Firmicutes were the most dominant groups of bacteria at different study sites. The bacterial diversity index of Species richness (S) and Margalef (d Ma ) were significantly higher in rhizosphere soil compared with those in bulk soil, although no difference could be found in Simpson index (D) between bulk and rhizosphere soils (p > 0.05). Redundancy analysis (RDA) results showed that soil pH, EC, SOM and total/DTPA-extractable Pb concentrations were the most important variables affecting relative abundance, composition and diversity of bacteria (p < 0.05). Our study highlights the importance of rhizosphere effect on soil nutrient content, enzyme activity, bacterial abundance and community in HM contaminated forest soils. Further study is still required to understand the specific processes in the rhizosphere to achieve a suitable rhizosphere biotechnology for restoration of degraded forest ecosystem.

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Arbuscular mycorrhizal fungi play a role in protecting roots of Sophora viciifolia Hance. from Pb damage associated with increased phytochelatin synthase gene expression

Cited by 16 publications

References 78 publications

The Combined Effects of Arbuscular Mycorrhizal Fungi (AMF) and Lead (Pb) Stress on Pb Accumulation, Plant Growth Parameters, Photosynthesis, and Antioxidant Enzymes in Robinia pseudoacacia L.

The Combined Effects of Arbuscular Mycorrhizal Fungi (AMF) and Lead (Pb) Stress on Pb Accumulation, Plant Growth Parameters, Photosynthesis, and Antioxidant Enzymes in Robinia pseudoacacia L.

The roles of arbuscular mycorrhizal fungi (AMF) in phytoremediation and tree-herb interactions in Pb contaminated soil

Comparisons of Soil Properties, Enzyme Activities and Microbial Communities in Heavy Metal Contaminated Bulk and Rhizosphere Soils of Robinia pseudoacacia L. in the Northern Foot of Qinling Mountain

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