Arbuscular Mycorrhizal Fungi Associated with Bisbul (Diospyros blancoi)

Abstract

() is a kind of edible fruit and could be beneficial as inhibitor for skin ageing process. Majority of root plants have symbiotic associations with arbuscular mycorrhizal fungi (AMF); however, AMF in has never been reported. The objective of this study was to observe AMF colonies and to identify AMF spores in bisbul tree rhizospheres and in potcultured . Roots and soil samples from rhizospheres were collected from three locations in Bogor area. Roots were stained using trypan blue 0.05%. Soil samples were air dried, then parts of them were used for spore isolation and the rest were used for pot cultures. Spores were isolated by wet sieving and decanting method and then continued with centrifugation. The results showed that the structures of AMF colonies in roots were arbuscules, vesicles, coiled hyphae, and internal hyphae. Isolated AMF spores were identified as , .and . This is the first report of mycorrhizal infection in the root of and AMF association with root.AM Fungi, symbiosis,