Arbuscular mycorrhizal fungal community structure and diversity in response to long-term fertilization: a field case from China

Abstract

The influences of different fertilizer treatments on spore community structure and diversity of arbuscular mycorrhizal (AM) fungi (AMF) were investigated in a long-term fertilization experiment with seven treatments: organic manure (OM), half organic manure N plus half fertilizer N (1/2 OMN), fertilizer NPK, fertilizer NP, fertilizer NK, fertilizer PK, and the control (without fertilization). Fertilization generally increased the nutrient contained in the fertilizer and treatments with NPK and 1/2 OMN produced the highest crop yields. Thirty-five species of AMF within 6 genera, including 8 previously undescribed species, were recovered. Similarly in all seven treatments, the most abundant genus was Glomus, and followed by Acaulospora. All the fertilization treatments changed AM species composition, and NK treatment had the slightest influence. Fertilization with fertilizers NP, PK and NPK markedly increased AM fungal spore density, while 1/2 OMN, OM and NK treatments showed no significant influences. All the fertilizer treatments, especially OM, significantly decreased species richness and species diversity (Shannon-Weiner index). There were no significant correlations between AM fungal parameters (spore density, species richness and species diversity) and soil properties. The findings indicate that long-term fertilization all can change AM fungal community structure and decrease species diversity, while balanced fertilization with NPK or 1/2 OMN is the most suitable fertilization regime if taking both crop yields and AM species diversity into account.

Arbuscular mycorrhizal fungal species composition, propagule density, and soil alkaline phosphatase activity in response to continuous and alternate no-tillage in Northern China

“…A total of 30 AM fungal species was recorded in this study, slightly lower than the number of 35 reported from the same arable land (Wang et al, 2011a). The genus Acaulospora registered the largest species number, while Acaulospora denticulata was the most abundant species with a relative abundance (RA) of 21.8-25.1% (Table 3).…”

“…previous studies (Wang et al, 2011a;Yang et al, 2011). Spore specimens identified to species were used in the analysis of the relative abundance (RA) and the species richness (SR).…”

Microbial Response to Soil Liming of Damaged Ecosystems Revealed by Pyrosequencing and Phospholipid Fatty Acid Analyses

“…Therefore, some of these PLFAs identified in fungi especially in limed sites, might be from plant materials. In general, 16:1ω5 and 18:1ω7 are good indictors of AM fungi [41,42]. Both PLFAs were slightly more abundant in limed sites compared to unlimed samples.…”

Nitrogen and phosphorus additions impact arbuscular mycorrhizal abundance and molecular diversity in a tropical montane forest

“…Previous studies report lower AMF species richness because of N (Egerton-Warburton et al, 2007;Wang et al, 2011) and also P fertilization (Alguacil et al, 2010;Gosling et al, 2013). Previous studies report lower AMF species richness because of N (Egerton-Warburton et al, 2007;Wang et al, 2011) and also P fertilization (Alguacil et al, 2010;Gosling et al, 2013).…”