Arbuscular mycorrhiza alters the nutritional requirements in Salvia miltiorrhiza and low nitrogen enhances the mycorrhizal efficiency

Abstract

Salvia miltiorrhiza Bunge (danshen in Chinese) is one of the most important medicinal cash crops in China. Previously, we showed that arbuscular mycorrhizal fungi (AMF) can promote S. miltiorrhiza growth and the accumulation of bioactive compounds. Fertilization may affect mycorrhizal efficiency, and appropriate doses of phosphate (P) and nitrogen (N) fertilizers are key factors for obtaining mycorrhizal benefits. However, the optimal fertilization amount for mycorrhizal S. miltiorrhiza remains unclear. In this study, we studied the effects of AMF on the growth and bioactive compounds of S. miltiorrhiza under different doses (low, medium, and high) of P and N fertilizer. The results showed that the mycorrhizal growth response (MGR) and mycorrhizal response of bioactive compounds (MBC) decreased gradually with increasing P addition. Application of a low (N25) dose of N fertilizer significantly increased the MGR of mycorrhizal S. miltiorrhiza, and a medium (N50) dose of N fertilizer significantly increased the MBC of phenolic acids, but decreased the MBC of tanshinones. Our results also showed that the existence of arbuscular mycorrhiza changes nutrient requirement pattern of S. miltiorrhiza. P is the limiting nutrient of non-mycorrhizal plants whereas N is the limiting nutrient of mycorrhizal plants.

Use of the Biostimulant Based on the Mycorrhizae Consortium of the <i>Glomeraceae</i> Family in the Field to Improve the Production and Nutritional Status of Maize (<i>Zea mays</i> L.) Plants in Central Benin

“…This caused a reduction in the performance recorded in Gbanlin, although not significant, would be due to the initial quality of the Gbanlin soil because it is the poorest in C/N, exchangeable Ca, Mg, K, Na and cationic sum followed by the Minifi soil (Table 1). This observation was proved by [55] that the rational fertility of the soil is conducive to maximize the benefits of mycorrhization. This was explained by [56] that a nitrogen-mycorrhiza interaction makes the plant vigorous and naturally protected.…”

Impacts of fertilization methods on Salvia miltiorrhiza quality and characteristics of the epiphytic microbial community

“…(2023) reported that fertilization can significantly alter the rhizosphere microbial community, leading to improved biomass and medicinal quality of S. miltiorrhiza . Pu et al. (2022) found that different fertilization regimes can impact mycorrhizal symbiosis, thereby positively affecting the growth and active ingredient content of S. miltiorrhiza .…”

“…For instance, Wei et al (2023) reported that fertilization can significantly alter the rhizosphere microbial community, leading to improved biomass and medicinal quality of S. miltiorrhiza. Pu et al (2022) found that different fertilization regimes can impact mycorrhizal symbiosis, thereby positively affecting the growth and active ingredient content of S. miltiorrhiza. Despite these findings, there are still gaps in the overall understanding of how the salvia quality is associated with the structure of the epiphytic microbial community under different fertilization strategies.…”