Arbovirus Isolations from Mosquitoes Collected during and after the 1982–1983 Epizootic of Western Equine Encephalitis in Argentina

Cj, Mitchell; Tp, Monath; Ms, Sabattini; Jf, Daffner; Cb, Cropp; Calisher, C. H.; Rf, Darsie; Wl, Jakob

doi:10.4269/ajtmh.1987.36.107

Cited by 78 publications

(54 citation statements)

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“…Other studies conducted in province of Chaco (Argentina) documented the circulation of arbovirus transmitted by mosquitoes (Mitchell et al 1985, 1987, Pisano et al 2010). However, no systematic studies of mosquito larval habitats have been conducted in this province.…”

mentioning

confidence: 99%

Classification of immature mosquito species according to characteristics of the larval habitat in the subtropical province of Chaco, Argentina

Stein

Ludueña-Almeida

Willener

et al. 2011

Mem. Inst. Oswaldo Cruz

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mentioning

confidence: 99%

Classification of immature mosquito species according to characteristics of the larval habitat in the subtropical province of Chaco, Argentina

Stein

Ludueña-Almeida

Willener

et al. 2011

Mem. Inst. Oswaldo Cruz

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“…Supernatants to be tested for virus by plaque assay in Vero cell culture were poured into 1-dram screw-cap vials and stored at Ϫ70ЊC until tested as previously described. 21 The microfuge tubes containing pellets and the remaining supernatant were resuspended, then sonicated at 100 W for 10 sec. The tubes were centrifuged at 14,000 rpm for 2 min, and the supernatant was transferred to a new vial.…”

Section: Methodsmentioning

confidence: 99%

Detection of eastern equine encephalitis virus in infected mosquitoes using a monoclonal antibody-based antigen-capture enzyme-linked immunosorbent assay.

Brown

Mitchell²,

Nasci³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. Surveillance of mosquito populations for virus activity is not often performed by small, vector-control districts because they do not have the financial resources to use virus isolation, or newer methods such as the polymerase chain reaction. Consequently, development and refinements of rapid, sensitive, and simple enzyme-linked immunosorbent assays (ELISAs) applicable to a wide variety of public health settings are justified. We have developed an antigen-capture ELISA for the detection of eastern equine encephalitis (EEE) virus in mosquitoes that uses both monoclonal capture and detector antibodies. The sensitivity of this assay is 4.0-5.0 log 10 plaque-forming units/ml, which is comparable to previously published EEE antigen-capture assays developed with polyclonal antibody reagents. This test identifies only North American strains of EEE virus and does not react with either western equine encephalitis or Highlands J viruses. Test sensitivity was enhanced by sonicating mosquito pools, treating them with Triton X-100, and increasing the time and temperature of antigen incubation. The conversion of this ELISA to a monoclonal antibody-based format should result in a readily standardizable and transferable assay that will permit laboratories lacking virus isolation facilities to conduct EEE virus surveillance.

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“…This species is one of the most annoying in the region because of its insistent attacks on humans and domestic animals (Prosen et al, 1960), causing diseases and substantial economic losses (Ludueña Almeida, 1994). It is also a cause of concern from the point of view of sanitation, as this species has been involved in the transmission of the western equine encephalitis virus (Mitchell et al, 1987;Avilés et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Probable trajectories associated with the transport of Ochlerotatus albifasciatus during a strong wind event in Buenos Aires city (Argentina)

Bejarán¹,

Fischer²,

Garin³

et al. 2008

Meteorological Applications

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Because of its capacity to infect human beings and domestic animals with a number of different viruses, the study of the mosquito Ochlerotatus albifasciatus (Macquart, 1938, (Diptera: Culicidae)) assumes great importance from the point of view of sanitation. It is a well-known fact that the abundance of this species peaks after drought periods in the provinces of Buenos Aires and Cordoba. This article aims at explaining the presence of the high abundance of adult O. albifasciatus in Buenos Aires city in February 2005. As no breeding immatures were recorded during the previous weeks, the most likely hypothesis was that mosquitoes entered the city from breeding sites located in the outskirts. Wind observations, the existing knowledge on this species biology, atmospheric dynamic models, and meteorological data were used to support this hypothesis and identify the sources.Mass transport of these individuals was apparently the consequence of a convective process. It was demonstrated that the occurrence of a convective process transported air masses from the periphery towards the City of Buenos Aires. Air-parcel trajectories, Ezeiza radar images, and the flood zones observed in satellite images indicated that the source was located in the lowlands of Ezeiza in the Rio Matanza basin, some 50 km to the southwest of the City. The evidence suggests that O. albifasciatus is transported by wind, and the trajectory models are a very useful tool to locate the sources of proliferation of these insects.

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Arbovirus Isolations from Mosquitoes Collected during and after the 1982–1983 Epizootic of Western Equine Encephalitis in Argentina

Cited by 78 publications

References 0 publications

Classification of immature mosquito species according to characteristics of the larval habitat in the subtropical province of Chaco, Argentina

Classification of immature mosquito species according to characteristics of the larval habitat in the subtropical province of Chaco, Argentina

Detection of eastern equine encephalitis virus in infected mosquitoes using a monoclonal antibody-based antigen-capture enzyme-linked immunosorbent assay.

Probable trajectories associated with the transport of Ochlerotatus albifasciatus during a strong wind event in Buenos Aires city (Argentina)

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