Arbovirus circulation among febrile patients at the greater Accra Regional Hospital, Ghana

Manu, Simon Kofi; Bonney, Joseph Humphrey Kofi; Pratt, Deborah; Abdulai, Farida Njelba; Agbosu, Esinam; Frimpong, Prince Osei; Adiku, Theophilus

doi:10.1186/s13104-019-4378-x

Cited by 18 publications

(18 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With no statistical associations, the most commonly reported clinical symptoms among the chikungunya and malaria infections aside from the febrile condition were headache, muscular pains, chills, shivering & sweating joint pains, and nausea/vomiting. This result is in line with that of other studies [22,26,29,31]. The current study did not record any positive signal for either chikungunya virus, dengue virus, or Zika virus RNA detection by RT-qPCR-a similar observation was reported here in Ghana by Manu et al, [22].…”

Section: Discussionsupporting

confidence: 94%

“…This result is in line with that of other studies [22,26,29,31]. The current study did not record any positive signal for either chikungunya virus, dengue virus, or Zika virus RNA detection by RT-qPCR-a similar observation was reported here in Ghana by Manu et al, [22]. Dash et al, (2011) pointed out that the detection of chikungunya viral RNA is from the day of infection or onset of symptoms to day 7, after which acute infections detection is limited [36].…”

Section: Discussionsupporting

confidence: 93%

“…IgM and IgG seropositivities were 1.8% and 4.2% respectively. The overall CHIKV specific seropositivity reported in the current study was lower than the 27.69% reported by Manu et al, (2019) separate and independent studies conducted in different parts of Nigeria. However, the overall low CHIKV antibodies seropositivity observed in the current study was comparable to that (4.1%) reported by Akinola et al, (2017) in Nigeria [25].…”

Section: Discussioncontrasting

confidence: 90%

See 2 more Smart Citations

Evidence of chikungunya virus infections among febrile patients at three secondary health facilities in the Ashanti and the Bono Regions of Ghana

et al. 2021

View full text Add to dashboard Cite

Background Chikungunya is now of public health concern globally due to its re-emergence in endemic areas and introduction into new areas of the world. Worldwide, the vectors for transmission of the chikungunya virus are Aedes mosquitoes and these are prevalent in Ghana. Despite its global significance, the true burden of chikungunya virus infection in Ghana is largely unknown and the threat of outbreak remains high owing to international travel. This study sought to determine chikungunya virus infection among febrile patients suspected of having malaria infections at some selected health facilities in the Ashanti, Bono East, and Bono Regions of Ghana. Methodology This cross-sectional study recruited six hundred (600) febrile patients suspected of having malaria who submitted their clinical samples to the laboratories of the selected health facilities for the diagnosis of their infections. Five to ten millilitres (5-10ml) of venous blood were collected from each study participant. Sera were separated and tested for anti-chikungunya (IgM and IgG) antibodies using InBios ELISA kit following the manufacturer’s instruction. Samples positive for chikungunya IgM and IgG were selected and tested for chikungunya virus RNA using Reverse Transcription-quantitative Polymerase Chain Reaction. Malaria Rapid Diagnostic Test kits were used to screen the participants for malaria. Structured questionnaires were administered to obtain demographic and clinical information of the study participants. Result Of the 600 samples tested, the overall seroprevalence of chikungunya was 6%. The seroprevalence of chikungunya IgM and IgG antibodies were 1.8% and 4.2% respectively. None of the chikungunya IgM and IgG positive samples tested positive for chikungunya RNA by RT-qPCR. Of the 600 samples, tested 32.3% (194/600) were positive for malaria parasites. Malaria and chikungunya co-infection was detected in 1.8% (11/600) of the participants. Conclusion Findings from the current study indicate low-level exposure to the chikungunya virus suggesting the virus is circulating and potentially causing morbidity in Ghana.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 93%

Section: Discussioncontrasting

confidence: 90%

See 1 more Smart Citation

Evidence of chikungunya virus infections among febrile patients at three secondary health facilities in the Ashanti and the Bono Regions of Ghana

et al. 2021

View full text Add to dashboard Cite

show abstract

“…aegypti populations confirmed the autochthonous urban transmission of dengue in urban cities as Ouagadougou. They were more or least overlapping with human infections patterns where DENV-2 and DENV-3 serotypes were the most detected in humans [29][30][31][32] but exceptionally DENV-4 was reported by Ridde et al [23] together with DENV-2 and DENV-3. The presence of DENV-2 was also found in the blood of febrile travellers returning from Burkina Faso to France during this period [24].…”

Section: Discussionmentioning

confidence: 73%

“…Aegypti [13]. Arboviruse's epidemiology in Africa during the past five years shows an expansion of some arboviruses such as West Nile, Chikungunya, Zika in West Africa [33][34][35].…”

Section: Discussionmentioning

confidence: 99%

Chikungunya (Togaviridae) and Dengue 2 (Flaviviridae) Viruses Isolated from Aedes aegypti Mosquitoes by qRT-PCR Technique: Xenosurveillance for Arboviruses Circulating in Burkina Faso

Hien¹,

Sangaré²,

Ouattara³

et al. 2021

Preprint

View full text Add to dashboard Cite

In 2016, we conducted an entomological survey in a railway transect between Banfora and Ouagadougou, Burkina Faso. The aim was to evaluate the risk factors for arbovirus epidemics, including vector infection status, in areas representative of the country. Aedes aegypti mosquitoes were collected at larval stage from four study sites and reared until adult stage and kept in RNAlater for detection of arbovirus RNA. In the laboratory, the mosquito specimens were screened for dengue virus (DENV) and chikungunya virus (CHIKV) using one step real-time qRT-PCR. We detected one DENV-2 positive pool from Ouagadougou, giving a minimum infec-tion rate (MIR) of 16.67, and 6 CHIKV positive pools, giving a MIR of 66.67 from Ouagadougou, but also in Banfora and Boromo. The qRT-PCR is a useful tool for the surveillance of arboviruses of public health importance in Burkina Faso and may be incorporated into disease surveillance and control programs in Burkina Faso.

show abstract

Tracing and tracking the emergence, epidemiology and dispersal of dengue virus to Africa during the 20th century

et al. 2021

View full text Add to dashboard Cite

The four mosquito-borne dengue virus serotypes (DENV1–DENV4) cause a high burden of disease throughout the tropical and sub-tropical regions of the world. Nevertheless, their precise epidemiological history in Africa, including when and where they originated and were distributed during the 20th century, remains unclear stressing the need for One Health focused research. Accordingly, we conducted a time-scaled molecular epidemiological reconstruction using publicly available and newly sequenced dengue virus genomes of African origin representing all four serotypes to deduce the most likely temporal and spatial transmission routes of each DENV serotype from their ancestral regions to, within and from Africa. Our analyses suggest that during the 20th century, serotypes DENV1–DENV3 were introduced to Africa from South East Asia on multiple occasions. The earliest evidence recorded indicates introduction of DENV2 during the early-1940s and of DENV1 during the mid-1940s to Western Africa from South East Asia. The analysis also implies an early introduction of DENV4 during the mid-1940s to Western Africa, alongside DENV1, probably originating in South East Asia. Establishment of DENV3 in Africa appears to have occurred later in the 1960s, apparently originating from South East Asia. However, with the re-establishment of DENV in the Americas, following the cessation of the PAHO mosquito control programme during the mid-20th century, evidence of introductions of DENV1 and DENV2 from the Americas to Western Africa was also observed. The data also identify intra-regional circulation of DENV, but also inter-regional dispersal of all four serotypes within Africa, which has led to a high degree of geographical overlap among serotypes. It is also noteworthy that DENV from both Eastern and Western Africa, have been introduced into Central Africa but there is no support for the converse relationship. For serotypes DENV1–DENV3, we observed probable exports from within established African DENV clusters (≥2 sequences) primarily to Eastern and Southern Asia. Collectively, our findings support the view that all DENV serotypes, apart from DENV4, have been introduced on multiple occasions to Africa, primarily originating from South East Asia, and subsequently to neighbouring regions within Africa.

show abstract

Arbovirus circulation among febrile patients at the greater Accra Regional Hospital, Ghana

Cited by 18 publications

References 21 publications

Evidence of chikungunya virus infections among febrile patients at three secondary health facilities in the Ashanti and the Bono Regions of Ghana

Evidence of chikungunya virus infections among febrile patients at three secondary health facilities in the Ashanti and the Bono Regions of Ghana

Chikungunya (Togaviridae) and Dengue 2 (Flaviviridae) Viruses Isolated from Aedes aegypti Mosquitoes by qRT-PCR Technique: Xenosurveillance for Arboviruses Circulating in Burkina Faso

Tracing and tracking the emergence, epidemiology and dispersal of dengue virus to Africa during the 20th century

Contact Info

Product

Resources

About