Árbol filogenético y características de bacterias endófitas asociadas a Theobroma cacao L. en una zona de la provincia de Esmeraldas, Ecuador

Vera-Loor, María Aracely; Bernal-Cabrera, Alexander; Vera-Coello, Danilo; Leiva-Mora, Michel; Rivero-Aragón, Alán; Villegas, Lisbeth Morales-Díaz de

doi:10.51372/bioagro333.8

Cited by 3 publications

References 9 publications

(6 reference statements)

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Implications of Domestication in Theobroma cacao L. Seed-Borne Microbial Endophytes Diversity

Toloza-Moreno,

Yockteng,

Pérez-Zuñiga

et al. 2024

Microb Ecol

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The study of plant–microbe interactions is a rapidly growing research field, with increasing attention to the role of seed-borne microbial endophytes in protecting the plant during its development from abiotic and biotic stresses. Recent evidence suggests that seed microbiota is crucial in establishing the plant microbial community, affecting its composition and structure, and influencing plant physiology and ecology. For Theobroma cacao L., the diversity and composition of vertically transmitted microbes have yet to be addressed in detail. We explored the composition and diversity of seed-borne endophytes in cacao pods of commercial genotypes (ICS95, IMC67), recently liberated genotypes from AGROSAVIA (TCS01, TCS19), and landraces from Tumaco (Colombia) (AC9, ROS1, ROS2), to evaluate microbial vertical transmission and establishment in various tissues during plant development. We observed a higher abundance of Pseudomonas and Pantoea genera in the landraces and AGROSAVIA genotypes, while the commercial genotypes presented a higher number of bacteria species but in low abundance. In addition, all the genotypes and plant tissues showed a high percentage of fungi of the genus Penicillium. These results indicate that domestication in cacao has increased bacterial endophyte diversity but has reduced their abundance. We isolated some of these seed-borne endophytes to evaluate their potential as growth promoters and found that Bacillus, Pantoea, and Pseudomonas strains presented high production of indole acetic acid and ACC deaminase activity. Our results suggest that cacao domestication could lead to the loss of essential bacteria for seedling establishment and development. This study improves our understanding of the relationship and interaction between perennial plants and seed-borne microbiota.

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Implications of Domestication in Theobroma cacao L. Seed-Borne Microbial Endophytes Diversity

Toloza-Moreno,

Yockteng,

Pérez-Zuñiga

et al. 2024

Microb Ecol

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Implications of Domestication in Theobroma Cacao L. Seed-borne Microbial Endophytes Diversity

Toloza-Moreno,

Yockteng,

Zuñiga

et al. 2024

Preprint

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The study of plant-microbe interactions is a rapidly growing research field, with increasing attention to the role of seed-borne microbial endophytes in protecting the plant during its development from abiotic and biotic stresses. Recent evidence suggests that seed microbiota is crucial in establishing the plant microbial community, affecting its composition and structure, and influencing plant physiology and ecology. For Theobroma cacao L., the diversity and composition of vertically transmitted microbes have yet to be addressed in detail. We explored the composition and diversity of seed-borne endophytes in cacao pods of commercial genotypes (ICS95, IMC67), AGROSAVIA genotypes (TCS01, TCS19), and landraces from Tumaco (Colombia) (AC9, ROS1, ROS2), to evaluate microbial vertical transmission and establishment in various tissues during plant development. We observed a higher abundance of Pseudomonas and Pantoea genera in the landraces and AGROSAVIA genotypes, while the commercial genotypes presented a higher number of bacteria species but in low abundance. In addition, all the genotypes and plant tissues showed a high percentage of fungi of the genus Penicillium. These results indicate that domestication in cacao has increased bacterial endophyte diversity but has reduced their abundance. We isolated some of these seed-borne endophytes to evaluate their potential as growth promoters and found that Bacillus, Pantoea, and Pseudomonas strains presented high production of indole acetic acid and ACC deaminase activity. Our results suggest that cacao domestication could lead to the loss of essential bacteria for seedling establishment and development. This study improves our understanding of the relationship and interaction between perennial plants and seed-borne microbiota.

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Bacillus spp. endófitos promueven diferencialmente el crecimiento de tres variedades de zarzamora (Rubus subgénero Eubatus)

Rojas-Sánchez

Santoyo

Delgado-Valerio

et al. 2022

Bioagro

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El género Bacillusproduce compuestos volátiles, hormonas vegetales, polisacáridos y enzimas relacionadas con el metabolismo de los fenilpropanoides, lo que representa un alto potencial para la promoción del crecimiento vegetal. En este estudio se analizaron los efectos de compuestos difusibles y volátiles producidos por cuatro endófitos bacterianos de Bacillus(Bacillussp. E25, B. toyonensisCOPE52, B. thuringiensisUM96 y Bacillussp. CR71) sobre la promoción del crecimiento de plántulas de zarzamora(Rubus fruticosus L.), cultivares Tupy, Kiowa y UM-13,mediante cultivo in vitro.Los resultados demostraron que la emisión de compuestos difusibles y orgánicos volátiles por la cepa COPE52 promovieron el aumento de la longitud y peso seco de la raíz, número de raíces y la concentración de clorofila en plántulas del cultivar Tupy. La emisión de compuestos difusibles de la cepa COPE52 indujo el aumento del peso seco de raíz y aéreo, y longitud de la raíz en el cultivar Kiowa, mientras que los compuestos volátiles emitidos porla cepa E25 promovieron incrementos en la mayoría de las variables analizadas en esta misma variedad. Los compuestos difusibles y volátiles producidos por la cepa CR71 tuvieron un mayor efecto sobre el número, longitud y peso seco de raíz en las plántulasdel cultivar UM-13, mientras que la concentración de clorofila aumentó con los compuestos producidos por la cepa E-25. En conclusión, las bacterias endófitas de Bacillusspp. promovieron diferencialmente el crecimiento de plántulas de los cultivares de zarzamora, en función del tipo de cepa inoculada y del modo de acción de los compuestos producidos.

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Árbol filogenético y características de bacterias endófitas asociadas a Theobroma cacao L. en una zona de la provincia de Esmeraldas, Ecuador

Cited by 3 publications

References 9 publications

Implications of Domestication in Theobroma cacao L. Seed-Borne Microbial Endophytes Diversity

Implications of Domestication in Theobroma cacao L. Seed-Borne Microbial Endophytes Diversity

Implications of Domestication in Theobroma Cacao L. Seed-borne Microbial Endophytes Diversity

Bacillus spp. endófitos promueven diferencialmente el crecimiento de tres variedades de zarzamora (Rubus subgénero Eubatus)

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