Arbitrarily Primed‐Polymerase Chain Reaction for Identification and Epidemiologic Subtyping of Oral Isolates of <i>Fusobacterium nucleatum</i>

Ávila-Campos, Mário Júlio; Sacchi, Cláudio Tavares; Whitney, Anne M.; Steigerwalt, Arnold G.; Mayer, Leonard W.

doi:10.1902/jop.1999.70.10.1202

Cited by 30 publications

(26 citation statements)

References 34 publications

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“…PCR amplification was performed using a thermalcycler (Perkin Elmer Cetus, Wellesley, MA, USA). The primer sequences and the PCR procedure were previously described with details by Avila-Campos et al 4 (1999) (F. nucleatum), Saiki et al 14 …”

Section: Methodsmentioning

confidence: 99%

Clinical and microbial evaluation of dental scaling associated with subgingival minocycline in chronic periodontitis subjects

et al. 2004

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ABSTRACT:The aims of this double-blind randomized clinical trial were to evaluate the presence of periodontal pathogens and the clinical response of periodontal pockets treatment to scaling and root planing (SRP) associated with subgingival minocycline (SM). A total of 36 subjects, 26 to 60 years old (40.7 ± 9.1), who had been previously diagnosed with chronic periodontitis, were included in the present study. Eighteen subjects were selected for the test group (TG), who were treated with SRP plus SM (new treatment), and 18 subjects for the control group (CG) who received SRP plus vehicle (current treatment). Two homologous sites in each subject with a probing depth (PD) ≥ 6 mm were chosen. To evaluate the clinical response after treatment, PD was measured at baseline and at 90 days. Microbiological evaluation was performed to detect 7 periodontal pathogens using polymerase chain reaction at baseline, 30, and 120 days. A mean reduction in PD of 2.8 and 2.1 mm was observed in the TG and CG, respectively. At baseline, P. gingivalis was the most prevalent organism in both test (65.8%) and control (48.6%) groups. After 120 days it fell to 30.8% in TG and to 23.1% in CG. There were no statistically significant differences between the test and control groups concerning PD (p > 0.05 by Wilcoxon test) or presence of periodontal pathogens (p > 0.05 by Wilcoxon and chi-square; p > 0.01 by Signal test). The results observed showed that the new treatment was as effective as the current treatment in reducing periodontal pathogens and PD among chronic periodontitis subjects. DESCRIPTORS: Bacteria; Dental scaling; Minocycline. RESUMO:O objetivo deste estudo randomizado duplo-cego foi avaliar a presença de periodontopatógenos e o comportamento clínico de bolsas periodontais tratadas com raspagem e aplainamento radicular (RAR) associado à minociclina subgengival (MS). Foram incluídos no presente estudo 36 indivíduos de 26 a 60 anos de idade (40,7 ± 9,1), previamente diagnosticados com periodontite crônica. Dezoito indivíduos foram selecionados para o grupo teste (GT), tratado com RAR e MS (novo tratamento), e 18 indivíduos, para o grupo controle (GC), que recebeu RAR e veículo (tratamento convencional). Foram selecionados dois sítios homólogos em cada indivíduo com profundidade de sondagem (PS) ≥ 6 mm para testar a hipótese proposta. Para avaliar o comportamento clí-nico após o tratamento, a mensuração da PS foi realizada inicialmente e após 90 dias. A avaliação microbiana foi realizada para detecção de 7 periodontopatógenos através de reação em cadeia da polimerase, inicialmente e após 30 e 120 dias. Observou-se redução média de 2,8 e 2,1 mm na PS nos grupos teste e controle, respectivamente. Inicialmente, P. gingivalis foi o microrganismo mais prevalente tanto no GT (65,8%) quanto no GC (48,6%). Após 120 dias, houve redução para 30,8% no GT e 23,1% no GC. Não houve diferenças estatisticamente significativas entre os grupos em relação à PS (teste Wilcoxon -p > 0,05) e presença dos periodontopatógenos (teste Wilcoxon e qui-quadra...

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Section: Methodsmentioning

confidence: 99%

Clinical and microbial evaluation of dental scaling associated with subgingival minocycline in chronic periodontitis subjects

et al. 2004

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“…Polymerase chain reaction (PCR) has been used in the direct identification of periodontal pathogens from subgingival specimens due to ability to accurately detect microbial species in mixed populations (3,4).…”

Section: Introductionmentioning

confidence: 99%

Detection of putative periodontal pathogens in subgingival specimens of dogs

Nishiyama¹,

Senhorinho²,

Gioso³

et al. 2007

Braz. J. Microbiol.

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In this study, the presence of putative periodontal organisms, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, Fusobacterium nucleatum, Dialister pneumosintes, Actinobacillus actinomycetemcomitans, Campylobacter rectus, Eikenella corrodens and Treponema denticola were examined from subgingival samples of 40 dogs of different breeds with (25) and without (15) periodontitis, by using the PCR method. The PCR products of each species showed specific amplicons. Of the 25 dogs with periodontitis, P. gingivalis was detected in 16 (64%) samples, C. rectus in 9 (36%), A. actinomycetemcomitans in 6 (24%), P. intermedia in 5 (20%), T. forsythensis in 5 (20%), F. nucleatum in 4 (16%) and E. corrodens in 3 (12%). T. denticola and D. pneumosintes were not detected in clinical samples from dogs with periodontitis. Moreover, P. gingivalis was detected only in one (6.66%) crossbred dog without periodontitis. Our results show that these microorganisms are present in periodontal microbiota of dogs with periodontitits, and it is important to evaluate the role of these putative periodontal microorganisms play in the periodontitis in household pets particularly, dogs in ecologic and therapeutic terms, since these animals might acquire these periodontopahogens from their respective owners.

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“…Polymerase chain reaction (PCR) has been used for direct identification of periodontal pathogens in subgingival specimens (3,11) and also for elucidating the role of specific bacteria in the periodontal disease because of the ability to accurately detect species in mixed populations. 16S rRNA gene amplification has been used for detection of microorganisms that cannot be cultivated but these methods have given crossreactions with related organisms (11).…”

Section: Introductionmentioning

confidence: 99%

PCR detection of four periodontopathogens from subgingival clinical samples

Ávila-Campos¹

2003

Braz. J. Microbiol.

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In this study, A. actinomycetemcomitans, B. forsythus, P. gingivalis and F. nucleatum were identified from subgingival plaque from 50 periodontal patients and 50 healthy subjects. Subgingival clinical samples were collected with sterilized paper points and transported in VMGA III. From all the diluted clinical samples (1:10), DNA was obtained by boiling, and after centrifugation the supernatant was used as template. Specific primers for each bacterial species were used in PCR. PCR amplification was sensitive to identify these organisms. PCR products from each species showed a single band and can be used to identify periodontal organisms from clinical specimens. PCR detection odds ratio values for A. actinomycetemcomitans and B. forsythus were significantly associated with disease showing a higher OR values for B. forsythus (2.97, 95% CI 1.88 -4.70). These results suggest a strong association among the studied species and the periodontal lesion.

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Arbitrarily Primed‐Polymerase Chain Reaction for Identification and Epidemiologic Subtyping of Oral Isolates of Fusobacterium nucleatum

Cited by 30 publications

References 34 publications

Clinical and microbial evaluation of dental scaling associated with subgingival minocycline in chronic periodontitis subjects

Clinical and microbial evaluation of dental scaling associated with subgingival minocycline in chronic periodontitis subjects

Detection of putative periodontal pathogens in subgingival specimens of dogs

PCR detection of four periodontopathogens from subgingival clinical samples

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