Arachidonic acid supplementation does not affect N-methyl-N-nitrosourea-induced renal preneoplastic lesions in young Lewis rats

Abstract

Arachidonic acid (AA) is naturally found in human breast milk. AA, together with docosahexaenoic acid, is commonly added as a functional food ingredient to commercial infant formula worldwide, in accordance with the international standards of Codex Alimentarius. However, few studies of the possible renal carcinogenic effects of AA supplementation during neonatal life have been performed. The effect of dietary AA supplementation in dams during gestation and lactation was investigated on N-methyl-N-nitrosourea (MNU)-induced preneoplastic lesions in the kidneys of young Lewis rats. Dams were fed a 2.0% AA diet or a basal diet (<0.01% AA). At birth (postnatal day 0), male and female pups received a single intraperitoneal injection of 35 mg/kg MNU or vehicle. Renal morphology was examined after 7, 14, 21, 28 and 60 days. Histopathologically, renal preneoplastic lesions, such as nephroblastomatosis and mesenchymal cell proliferation, were found on day 60 in both the MNU-treated groups. There was no significant difference in lesion incidence of 38% in the basal diet group and 31% in the AA diet group. In conclusion, an AA-rich diet for dams during gestation and lactation does not modify MNU-induced renal preneoplastic lesions in their offspring.

Chronic Arachidonic Acid Administration Decreases Docosahexaenoic Acid- and Eicosapentaenoic Acid-Derived Metabolites in Kidneys of Aged Rats

“…These results agree with those of our previous study [ 16 ]. Yoshizawa et al reported that an ARA-rich diet for dams during gestation and lactation does not modify N -methyl- N -nitrosourea-induced renal preneoplastic lesions in their offspring [ 25 ], indicating that exogenous free-form ARA does not induce inflammation or oxidative stress in the kidney.…”

<i>N</i>-Methyl-<i>N </i>-nitrosourea-induced Renal Tumors in Rats: Immunohistochemical Comparison to Human Wilms Tumors

“…Fourteen surviving female rats were used for the analysis at the age of 16 weeks. Rats were maintained under specific pathogen-free conditions and had free access to water and a CE-2 diet (CLEA Japan, Tokyo, Japan) according to a previous study 12 . Animals were housed in plastic cages with paper-chip bedding (Paper Clean, SLC, Hamamatsu, Japan) in an air-conditioned room at 22 ± 2°C and 60 ± 10% relative humidity with a 12-hr light/dark cycle.…”

“…Nitrosourea compounds including N -methyl- N -nitrosourea (MNU) also possess carcinogenic potency in the kidneys 8 , 9 , and MNU induces renal mesenchymal tumors (RMTs) and nephroblastomas in rats 10 , 11 , 12 . The formation and persistence of DNA adducts, such as O 6 -methylguanine, and K- ras codon 12 and 16 point mutations in renal cortical tubular cells and/or mesenchymal interstitial cells is related to the tumor development induced by alkylating agents 9 , 13 .…”

N-methyl-N-nitrosourea-induced cerebellar hypoplasia in rats: Effect of arachidonic acid supplementation during the gestational, lactational and post-weaning periods

“…As in previous studies, the AA-supplemented diet was formulated by CLEA Japan, Inc. (Tokyo, Japan) (9,10,31). AA was purchased from Cargill Alking Bioengineering LLC (Wuhan and Hubei, China).…”