Arachidonic Acid Pathways and Male Fertility: A Systematic Review

Hoxha, Malvina; Barbonetti, Arcangelo; Zappacosta, Bruno

doi:10.3390/ijms24098207

Cited by 4 publications

(1 citation statement)

References 95 publications

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“…Polyunsaturated fatty acids (PUFAs) play a crucial role in the biosynthesis of arachidonic acid (AA) and indirectly participate in the production of prostaglandin E 2 (PGE 2 ) [50,51]. PGE 2 has a significant impact on various physiological processes such as fluid secretion, immunity, ageing, and reproduction in insects, similar to vertebrates [52].…”

Section: Discussionmentioning

confidence: 99%

Validation of Diets with Tomato Pomace in Complete Cycle Breeding of Tenebrio molitor (L.) (Coleoptera: Tenebrionidae)

Baldacchino,

Spagnoletta,

Lamaj

et al. 2024

Insects

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By-product-based diets have the potential to improve the environmental and economic sustainability of Tenebrio molitor (Linnaeus, 1758) production. However, evaluations of the efficacy of new diets are generally focused on larval performance, while the effect on adults is poorly understood. This aim of this study was to evaluate diets enriched with tomato pomace over a complete breeding cycle. The results showed that when used as an oviposition substrate, all the tested diets, including tomato pomace (T), outperformed the control bran-yeast diet (WY, 95:5 ratio), possibly due to the presence of cholesterol and linoleic acid. The adults fed with the bran-tomato pomace-brewer’s spent grain diet (WTB, 50:27:23 ratio), the bran-tomato pomace-yeast diet (WTY, 50:41:9 ratio), and the bran-tomato pomace diet (WT, 50:50 ratio) produced significantly more larvae than those fed with the WY diet. The WTB diet (despite being yeast-free) performed similarly to the WY control diet during the subsequent larval growth phase, making it suitable for the entire production cycle. In conclusion, the results show that tomato pomace can be used a valid by-product in the formulation of efficient diets for the breeding of T. molitor and also provide an alternative to expensive yeast.

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Section: Discussionmentioning

confidence: 99%

Validation of Diets with Tomato Pomace in Complete Cycle Breeding of Tenebrio molitor (L.) (Coleoptera: Tenebrionidae)

Baldacchino,

Spagnoletta,

Lamaj

et al. 2024

Insects

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Multiple transcriptome analyses reveal mouse testis developmental dynamics

Chen,

Ji,

et al. 2024

BMC Genomics

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The testes are the organs of gamete production and testosterone synthesis. Up to date, no model system is available for mammalian testicular development, and only few studies have characterized the mouse testis transcriptome from no more than three postnatal ages. To describe the transcriptome landscape of the developing mouse testis and identify the potential molecular mechanisms underlying testis maturation, we examined multiple RNA-seq data of mouse testes from 3-week-old (puberty) to 11-week-old (adult). Sperm cells appeared as expected in 5-week-old mouse testis, suggesting the proper sample collection. The principal components analysis revealed the genes from 3w to 4w clustered away from other timepoints, indicating they may be the important nodes for testicular development. The pairwise comparisons at two adjacent timepoints identified 7,612 differentially expressed genes (DEGs), resulting in 58 unique mRNA expression patterns. Enrichment analysis identified functions in tissue morphogenesis (3-4w), regulation of peptidase activity (4-5w), spermatogenesis (7-8w), and antigen processing (10-11w), suggesting distinct functions in different developmental periods. 50 hub genes and 10 gene cluster modules were identified in the testis maturation process by protein-protein interaction (PPI) network analysis, and the miRNA-lncRNA-mRNA, miRNA-circRNA-mRNA and miRNA-circRNA-lncRNA-mRNA competing endogenous RNA (ceRNA) networks were constructed. The results suggest that testis maturation is a complex developmental process modulated by various molecules, and that some potential RNA-RNA interactions may be involved in specific developmental stages. In summary, this study provides an update on the molecular basis of testis development, which may help to understand the molecular mechanisms of mouse testis development and provide guidance for mouse reproduction.

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Identification of key metabolism-related genes and pathways in spontaneous preterm birth: combining bioinformatic analysis and machine learning

Lv,

Xie,

et al. 2024

Front. Endocrinol.

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BackgroundSpontaneous preterm birth (sPTB) is a global disease that is a leading cause of death in neonates and children younger than 5 years of age. However, the etiology of sPTB remains poorly understood. Recent evidence has shown a strong association between metabolic disorders and sPTB. To determine the metabolic alterations in sPTB patients, we used various bioinformatics methods to analyze the abnormal changes in metabolic pathways in the preterm placenta via existing datasets.MethodsIn this study, we integrated two datasets (GSE203507 and GSE174415) from the NCBI GEO database for the following analysis. We utilized the “Deseq2” R package and WGCNA for differentially expressed genes (DEGs) analysis; the identified DEGs were subsequently compared with metabolism-related genes. To identify the altered metabolism-related pathways and hub genes in sPTB patients, we performed multiple functional enrichment analysis and applied three machine learning algorithms, LASSO, SVM-RFE, and RF, with the hub genes that were verified by immunohistochemistry. Additionally, we conducted single-sample gene set enrichment analysis to assess immune infiltration in the placenta.ResultsWe identified 228 sPTB-related DEGs that were enriched in pathways such as arachidonic acid and glutathione metabolism. A total of 3 metabolism-related hub genes, namely, ANPEP, CKMT1B, and PLA2G4A, were identified and validated in external datasets and experiments. A nomogram model was developed and evaluated with 3 hub genes; the model could reliably distinguish sPTB patients and term labor patients with an area under the curve (AUC) > 0.75 for both the training and validation sets. Immune infiltration analysis revealed immune dysregulation in sPTB patients.ConclusionThree potential hub genes that influence the occurrence of sPTB through shadow participation in placental metabolism were identified; these results provide a new perspective for the development and targeting of treatments for sPTB.

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Arachidonic Acid Pathways and Male Fertility: A Systematic Review

Cited by 4 publications

References 95 publications

Validation of Diets with Tomato Pomace in Complete Cycle Breeding of Tenebrio molitor (L.) (Coleoptera: Tenebrionidae)

Validation of Diets with Tomato Pomace in Complete Cycle Breeding of Tenebrio molitor (L.) (Coleoptera: Tenebrionidae)

Multiple transcriptome analyses reveal mouse testis developmental dynamics

Identification of key metabolism-related genes and pathways in spontaneous preterm birth: combining bioinformatic analysis and machine learning

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