Arachidonic-acid metabolism in normal and experimentally-inflamed rat dental pulp

Gene Expression Analysis of Membrane Transport Proteins in Normal and Lipopolysaccharide-inflamed Rat Dental Pulp

“…Experimental pulp inflammation was induced by applying bacterial LPS (Escherichia coli 0.55:B5), according to the method previously described (11,12). Under pentobarbital sodium anesthesia (30 mg/kg, intraperitoneally), the crowns of upper incisors were cut off at the gingival margin, and the coronal pulp chamber was instrumented to size 45 and to 7 mm in length with stainless steel files.…”

“…Regarding the dental pulp, PGE 2 is considered an important modulator of inflammation, because PGE 2 synthesis increases in experimentally inflamed pulps of rats (11)(12)(13) and dogs (14), and PGE 2 levels in pulp blood samples are higher in inflamed pulps than in clinically intact teeth (15). NSAIDs reduce the increase of PGE 2 synthesis and vascular permeability in lipopolysaccharide (LPS)-stimulated rat incisor pulps (12), which might implicate PGE 2 in the increase of vascular permeability.…”

Orthodontic Adhesives Induce Human Gingival Fibroblast Toxicity and Inflammation

“…7 The prostaglandin E2 inflammatory mediator is known to exert diverse physiologic actions in different tissues and to be involved in the inflammation process. [8][9][10] The enzymes, including phospholipase A2 and cyclooxygenase (COX), regulate the production of the prostaglandin. Prostaglandins are produced by the action of COX enzymes on the free arachidonic acid liberated from membrane phospholipids by phospholipases.…”

Inhibition of Lipoxygenase by Phenolic compounds.

“…Lessard et al showed an increase in LTC4 level by in flammatory treatments in canine tooth pulp (6). Okiji et al demonstrated that arachidonic acid was converted to 12-HETE (12-hydroxy 5,8,10,14-eicosatetraenoic acid) by incubation with rat dental pulp homogenate and the con version increased in the inflamed pulp (7). The evidence suggests that the lipoxygenase system is present in dental pulp tissue and may be involved in the development of pulp inflammation.…”

“…The pulp was sonicated at 20 kHz for 15 sec in 2 vol. of 0.1 M potassium phosphate buffer (pH 7.4) and used as the enzyme preparation as previously reported (7). PMN were isolated from peripheral blood collected from healthy volunteers as described previously (8).…”