Arachidonic acid inhibits myelin basic protein phosphorylation in cultured oligodendrocytes

Takeda, Margaret; Soliven, Betty

doi:10.1002/(sici)1098-1136(199711)21:3<277::aid-glia3>3.0.co;2-2

Cited by 11 publications

(4 citation statements)

References 41 publications

(55 reference statements)

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“…38, 39, 40, 53, 54 Of the known NBIA genes, these have the strongest links to myelin and also showed the greatest changes in iron-loaded mouse brains. Products of pla2g6 activity are required for myelin homeostasis 55, 56, 57 and FA2H is crucial for biosynthesis of myelin lipid components. 58, 59 Patients with C19orf12 and ATP13A2 mutations show myelin reduction 8, 41, 60 and we observed decreased expression of these genes in iron-loaded mouse brains.…”

Section: Discussionmentioning

confidence: 99%

Brain iron accumulation affects myelin-related molecular systems implicated in a rare neurogenetic disease family with neuropsychiatric features

et al. 2016

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The ‘neurodegeneration with brain iron accumulation' (NBIA) disease family entails movement or cognitive impairment, often with psychiatric features. To understand how iron loading affects the brain, we studied mice with disruption of two iron regulatory genes, hemochromatosis (Hfe) and transferrin receptor 2 (Tfr2). Inductively coupled plasma atomic emission spectroscopy demonstrated increased iron in the Hfe−/− × Tfr2mut brain (P=0.002, n ≥5/group), primarily localized by Perls' staining to myelinated structures. Western immunoblotting showed increases of the iron storage protein ferritin light polypeptide and microarray and real-time reverse transcription-PCR revealed decreased transcript levels (P<0.04, n ≥5/group) for five other NBIA genes, phospholipase A2 group VI, fatty acid 2-hydroxylase, ceruloplasmin, chromosome 19 open reading frame 12 and ATPase type 13A2. Apart from the ferroxidase ceruloplasmin, all are involved in myelin homeostasis; 16 other myelin-related genes also showed reduced expression (P<0.05), although gross myelin structure and integrity appear unaffected (P>0.05). Overlap (P<0.0001) of differentially expressed genes in Hfe−/− × Tfr2mut brain with human gene co-expression networks suggests iron loading influences expression of NBIA-related and myelin-related genes co-expressed in normal human basal ganglia. There was overlap (P<0.0001) of genes differentially expressed in Hfe−/− × Tfr2mut brain and post-mortem NBIA basal ganglia. Hfe−/− × Tfr2mut mice were hyperactive (P<0.0112) without apparent cognitive impairment by IntelliCage testing (P>0.05). These results implicate myelin-related systems involved in NBIA neuropathogenesis in early responses to iron loading. This may contribute to behavioral symptoms in NBIA and hemochromatosis and is relevant to patients with abnormal iron status and psychiatric disorders involving myelin abnormalities or resistant to conventional treatments.

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Section: Discussionmentioning

confidence: 99%

Brain iron accumulation affects myelin-related molecular systems implicated in a rare neurogenetic disease family with neuropsychiatric features

et al. 2016

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“…Arachidonic acid has been proposed to play an important role in cultured oligodendrocytes where it inhibits phosphorylation of MBP, increases the level of [Ca 2+ ] i and inhibits potassium conductance (Soliven et al. , 1993; Soliven & Wang, 1995; Takeda & Soliven, 1997).…”

Section: Discussionmentioning

confidence: 99%

Mu‐ and delta‐opioid receptor antagonists decrease proliferation and increase neurogenesis in cultures of rat adult hippocampal progenitors

Persson

Thorlin

Bull

et al. 2003

Eur J of Neuroscience

105

100

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Opioids have previously been shown to affect proliferation and differentiation in various neural cell types. In the present study, cultured rat adult hippocampal progenitors (AHPs) were shown to release beta-endorphin. Membrane preparations of AHPs were found to bind [125I]beta-endorphin, and immunoreactivity for mu- and delta-opioid receptors (MORs and DORs), but not for kappa-opioid receptors (KORs), was found on cells in culture. Both DNA content and [3H]thymidine incorporation were reduced after a 48-h incubation with 100 microM naloxone, 10 micro m naltrindole or 10 microM beta-funaltrexamine, but not nor-binaltorphimine, suggesting proliferative actions of endogenous opioids against MORs and DORs on AHPs. Furthermore, analysis of gene and protein expression after incubation with MOR and DOR antagonists for 48 h using RT-PCR and Western blotting suggested decreased signalling through the mitogen-activated protein kinase (MAPK) pathway and lowered levels of genes and proteins that are important in cell cycling. Cultures were incubated with naloxone (10 or 100 microM) for 10 days to study the effects on differentiation. This resulted in an approximately threefold increase in neurogenesis, a threefold decrease in astrogliogenesis and a 50% decrease in oligodendrogenesis. In conclusion, this study suggests that reduced signalling through MORs and DORs decreases proliferation in rat AHPs, increases the number of in vitro-generated neurons and reduces the number of astrocytes and oligodendrocytes in culture.

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“…For instance, membrane depolarisation affects protein phosphorylation of myelin basic protein in oligodendrocytes, an important event in myelination (Takeda & Soliven, 1997).…”

Section: Polyunsaturated Fatty Acids (Pufas)mentioning

confidence: 99%

Antioxidants and polyunsaturated fatty acids in multiple sclerosis

Meeteren¹,

Teunissen

Dijkstra

et al. 2005

Eur J Clin Nutr

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Arachidonic acid inhibits myelin basic protein phosphorylation in cultured oligodendrocytes

Cited by 11 publications

References 41 publications

Brain iron accumulation affects myelin-related molecular systems implicated in a rare neurogenetic disease family with neuropsychiatric features

Brain iron accumulation affects myelin-related molecular systems implicated in a rare neurogenetic disease family with neuropsychiatric features

Mu‐ and delta‐opioid receptor antagonists decrease proliferation and increase neurogenesis in cultures of rat adult hippocampal progenitors

Antioxidants and polyunsaturated fatty acids in multiple sclerosis

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