Arachidonic acid facilitates experimental chronic osteomyelitis in rats

Rissing, J. Peter; Buxton, Thomas B.; Fisher, J. Edward; Harris, Rose; Shockley, Robert K.

doi:10.1128/iai.49.1.141-144.1985

Cited by 37 publications

(11 citation statements)

References 13 publications

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“…The rat has been a good model for studying intramedullary rodding (15), but it has not been a good model for studying infections as it is difficult to cause infections in rats. Only recently have there been reports indicating that the rat is suitable for studying osteomyelitis in the intact tibia using sodium morrhuate or arachidonic acid similar to that described using the rabbit (17,18). With the exception of these very recent reports, the infection rate, dose of organisms, and type of organisms causing infection are very different in the rat from those in human infections.…”

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confidence: 99%

Role of internal fixation in infection of open fractures: Studies with Staphylococcus aureus and Proteus mirabilis

Merritt

Dowd

1987

Journal Orthopaedic Research

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Studies of infections in open fractures are described. The hamster was chosen as the experimental model. Osteotomies of the femur were created with an osteotomy saw. Some osteotomies were left to heal and others were fixed with an 0.9 mm K-wire. The infection rates in fixed and unfixed fractures were compared. The first group of hamsters with fixed and unfixed osteotomies was returned to cages with open wounds. There was no difference in the infection rate at 2 weeks. The second group was deliberately contaminated with Staphylococcus aureus and then returned to cages with open wounds. In this group, the infection rate at 2 weeks was lower in the internally-fixed fractures than in the unfixed fractures. The third group was deliberately contaminated with the gram negative organism Proteus mirabilis. In these animals, the infection rate was increased in the presence of the internal fixation device. The fourth group was deliberately contaminated with both Staphylococcus aureus and Proteus mirabilis. The infection rate in these animals was very high; Proteus was recovered from those animals with internal fixation and Staphylococcus was recovered from those animals without internal fixation. These studies in the hamster document the usefulness of this animal as an inexpensive and reproducible model for studying infection of open fractures. The hamsters tolerated the procedure well, and wound and fracture healing progressed satisfactorily.

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confidence: 99%

Role of internal fixation in infection of open fractures: Studies with Staphylococcus aureus and Proteus mirabilis

Merritt

Dowd

1987

Journal Orthopaedic Research

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“…In previous animal osteomyelitis models, local injection of vascular sclerosing agent was used to simulate clinical ischemic symptoms. However, there are few patients with osteomyelitis caused by local injection of vascular sclerosing agent into bone tissue [12] . Therefore, there is a gap between the osteomyelitis model made by sclerosing agent and the actual incidence of osteomyelitis, which may lead to changes in the in ammatory pathological mechanism of the bone infection model, which is not conducive to the later study of the treatment of osteomyelitis.…”

Section: Discussionmentioning

confidence: 99%

“…Eighteen rats were randomly divided into 3 groups. Group A: femoral artery ligation + Kirschner needle with S. aureus bio lm internal xation; Group B (positive control, vascular sclerosing agent group [12] ): vascular sclerosing agent + Kirschner needle with S. aureus bio lm internal xation; Group C (blank control): noninfected aseptic operation group and sterile Kirschner needle internal xation.…”

Section: Experiments Groupingmentioning

confidence: 99%

Establishment of an animal model of chronic osteomyelitis with Staphylococcus aureus by ligating the femoral artery of rats

Feng

Yang

Wang

et al. 2020

Preprint

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Background Osteomyelitis caused by Staphylococcus aureus (S. aureus) is an important post-operation complication, especially after fracture internal fixation and artificial joint replacement. Animal models play an indispensable role in exploring the pathogenesis of osteomyelitis. Most models use internal fixation, bacterial suspension and vascular sclerosing agent to destroy blood vessels. Vascular sclerosing agents not only damage blood vessels but also lead to local inflammatory immune disorders, which is different from simple vascular disease and osteomyelitis caused by ischemia in clinical practice. Methods The experimental animals were randomly divided into three groups: femoral artery ligation group, vascular sclerosing agent group and non-infection aseptic operation group. In the femoral artery ligation group, the femoral artery was ligated to reduce the blood flow of the affected limb to simulate the clinical ischemic state and increase the susceptibility, then the Kirschner needle with S. aureus biofilm was implanted into the tibia of rats, and the bone defect was sealed with aseptic paraffin. The non-infection aseptic operation group and the infection model group caused by vascular sclerosing agent have been used as the blank and positive control group. After operation, survival rate, body temperature and incision healing were monitored. Four weeks later, radiological and pathological changes of all animals were evaluated, and the secretions from osteomyelitis experienced etiological separation and cultivation. Results The chronic osteomyelitis model was established successfully by ligating femoral artery and implanting Kirschner needle covered with S. aureus biofilm. Signs of chronic osteomyelitis were observed in all rats of femoral artery ligation infection group and positive control group. No signs of infection and chronic osteomyelitis were found in the non-infection aseptic operation control group. Conclusion The method of ligating the femoral artery and implanting Kirschner needle with S. aureus biofilm into the tibia of rats can effectively establish a stable and reproducible chronic osteomyelitis model which is closer to the clinical pathogenesis and natural route of infection. This model could be useful for the study of pathogenesis and therapeutics of chronic osteomyelitis with S. aureus.

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“…Different osteomyelitis experimental models have been proposed in the bibliography using a variety of strategies and agents to induce osseous infection. As an example, authors used morrhuate sodium or its derivative arachidonic acid to induce aseptic osseous necrosis, which is an ideal situation for bacterial proliferation [ 36 ]. The insertion of foreign bodies provides a surface for bacterial adhesion and further biofilm formation, resulting in an increase in bacterial resistance to the host defenses [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

In vivo bactericidal efficacy of the Ti6Al4V surface after ultraviolet C treatment

Constantino¹,

Delgado-Rastrollo

Pacha-Olivenza

et al. 2016

J Orthopaed Traumatol

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BackgroundBiomaterial-associated infections are one of the most important complications in orthopedic surgery. The main goal of this study was to demonstrate the in vivo bactericidal effect of ultraviolet (UV) irradiation on Ti6Al4V surfaces.Materials and methodsAn experimental model of device-related infections was developed by direct inoculation of Staphylococcus aureus into the canal of both femurs of 34 rats. A UV-irradiated Ti6Al4V pin was press-fit into the canal by retrograde insertion in one femur and the control pin was inserted into the contralateral femur. To assess the efficacy of UV radiation, the mean colony counts after inoculation in the experimental subjects and the control group were compared at different times of sacrifice and at different inoculum doses.ResultsAt 72 h, the mean colony counts after inoculation in experimental femurs were significantly lower than those of the control group, with a reduction percentage of 76 % (p = 0.041). A similar difference between control and experimental pins was observed at 24 h using an inoculum dose <104 colony-forming units (CFU), for which the reduction percentage was 70.48 % (p = 0.017).ConclusionThe irradiated surface of Ti6Al4V is able to reduce early bacterial colonization of Ti6AlV pins located in the medullar channel and in the surrounding femur. The reductions depend on the initial inoculums used to cause infection in the animals and the greatest effects are detected for inoculums <104 CFU.Level of evidenceNot applicable.

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Arachidonic acid facilitates experimental chronic osteomyelitis in rats

Cited by 37 publications

References 13 publications

Role of internal fixation in infection of open fractures: Studies with Staphylococcus aureus and Proteus mirabilis

Role of internal fixation in infection of open fractures: Studies with Staphylococcus aureus and Proteus mirabilis

Establishment of an animal model of chronic osteomyelitis with Staphylococcus aureus by ligating the femoral artery of rats

In vivo bactericidal efficacy of the Ti6Al4V surface after ultraviolet C treatment

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