Arabinoxylan from Argentinian whole wheat flour promote the growth of Lactobacillus reuteri and Bifidobacterium breve

Abstract

Arabinoxylans are part of dietary fibre and have received attention given their emergent prebiotic character. Four arabinoxylans extracts were obtained from Argentinian soft and hard wheat. In vitro assays were performed to describe the extent to which the extracts from whole wheat flour support selective growth of Bifidobacterium breve and probiotic Lactobacillus reuteri ATCC23272 in a defined media. The prebiotic effect was evaluated by three quantitative scores: relative growth, prebiotic activity score and prebiotic index. For prebiotic index equation the growth of Bacteroides and Clostridium strains was compared to that of bifidobacteria and lactic acid bacteria. All the arabinoxylans extracts supported the growth of Lactobacillus and Bifidobacterium, reaching higher prebiotic activity score values than inulin (0·37 and 0·36 for Lactobacillus and Bifidobacterium respectively). AX2 from soft wheat and AX4 from hard showed similar prebiotic index value to commercial inulin (2·64, 2·52 and 2·22 respectively), and AX3 extract presented higher prebiotic index value (4·09) than the positive control and other prebiotic index reported for arabinoxylans. These extracts could be used as prebiotic, synbiotic compositions or novel food prototypes to treat dysbiosis associated with many diseases. Significance and Impact of the Study The present work demonstrates that AX extracts from Argentinian soft and hard wheat promote efficiently the growth of probiotic strain L. reuteri ATCC23272 and B. breve 286, validated with three different parameters that consider the growth of representative strains of Bacteria genera found in the gut. The evaluation of AX extracts as a food supplement in a murine model could confirm their ability to modulate the microbiome. Novel food prototypes including AX and probiotics could relieve local symptoms and may act as psychobiotics with a beneficial effect on microbiome‐brain axis.

Fermentation of Ferulated Arabinoxylan Recovered from the Maize Bioethanol Industry

“…Crittenden et al [18] found that B. longum is more efficient than Bacteroides strains when using AX as a growth substrate. Additionally, evident growth of Bifidobacterium breve 286 on wheat AX was observed by Paesani et al [27]. The degradation of AX by Bifidobacteria was demonstrated to be strain-dependent [28] and susceptible to cross-feeding between two strains, as previously reported with B. longum and B. adolescentis [14].…”

Early life Western diet-induced memory impairments and gut microbiome changes in female rats are long-lasting despite healthy dietary intervention

“…arabinoxylan [corn, wheat], beta glucan [oat], cellulose [corn, alfalfa, wheat, beet], hemicellulose [corn, alfalfa, wheat, beet], pectin [beet] [55][56][57][58]. These different dietary fibers have been shown to serve as nutrient sources for a variety of gut microbial taxa, including the genus Bifidobacterium (cellulose, pectin, oat, arabinoxylan) [59][60][61][62][63], the genus Parasutterella (pectin) [63], the phylum Actinobacteria (pectin) [63], and the genera Bacteroides-Prevotella (oat, arabinoxylan) [59], among others. The present study was not designed to pinpoint the effects of specific dietary fibers (or lack thereof) on cognitive function or the microbiome, but this is an important area of future work.…”

Correlation between faecal microbial taxa and ulcerative colitis in different phases of disease activity in a north Indian cohort

“…This suggests that these “good” commensals can become “bad” and can cause unwarranted immune stimulation in genetically susceptible individuals reiterating the likely effect of host genetics on microbiome composition. Other than host genetics, differential selection pressure through diet 42,43 ; disease characteristics; and persistent use of 5-ASA (which changes the colonic luminal pH and hence promote growth of Bifidobacterium and Lactobacillus) 35 , could be the non-genetic factors that may have contributed on the gut microbial assemblage. Considering the global uniformity of treatment modalities it seems that effect of disease modifying drugs on expansion of these genera is minimal.…”