Arabinose substitution degree in xylan positively affects lignocellulose enzymatic digestibility after various NaOH/H2SO4 pretreatments in Miscanthus

Li, Fengcheng; Ren, Shuai; Zhang, Wei; Xu, Zhengdan; Xie, Guangyong; Chen, Yan; Tu, Yuanyuan; Li, Qing; Zhou, Shiguang; Yu, Li; Tu, Fen; Liu, Lin; Wang, Yanting; Jiang, Jianxiong; Qin, Jingping; Li, Shizhong; Li, Qiwei; Jing, Hai‐Chun; Zhou, Fangjian; Gutterson, Neal; Peng, Liangcai

doi:10.1016/j.biortech.2012.12.107

Cited by 117 publications

(148 citation statements)

References 35 publications

Supporting

Mentioning

136

Contrasting

Unclassified

Order By: Relevance

“…Ramie showed a relatively high cellulose level (41.5% dry matter) and low lignin (22.5%), whereas jute had relatively high hemicelluloses content (26.0%; Table A.1), and kenaf had low cellulose content (34.8%; Table A.1). However, all three fiber crops exhibited much higher cellulose levels than that reported for most grass plants (Xu et al 2012;Wu et al 2013;Li et al 2013;Jia et al 2014). Hence, the three fiber crops could provide cellulose-rich biomass in the core stalks.…”

Section: Extraction Of Wall Polymers With Steam Explosionmentioning

confidence: 82%

“…Furthermore, the hemicellulose content is negatively correlated with the degree of cellulose crystallinity for high biomass saccharification under H2SO4 and NaOH pretreatments in many plant species examined (Xu et al 2012;Zhang et al 2013;Wu et al 2013;Jia et al 2014). In particular, arabinose substitution degree of xylans (reverse Xyl/Ara) has been discovered as a positive factor relative to biomass digestibility upon various chemical pretreatments (Wu et al 2013;Li et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Monosaccharides of hemicelluloses were determined using GC-MS (GCMSQP2010 Plus, SHIMADZU Corporation, Japan) as previously described by Li et al (2013). TFA (Trifluoroacetic acid) and myo-inositol were obtained from Aladdin Reagent Inc. Acetic anhydride and monosaccharide standards (L-rhamnose, L-arabinose, L-fucose, D-xylose, D-galactose, Dglucose, and D-mannose) were purchased from Sinopharm Chemical Reagent Co., Ltd. 1-Methylimidazole was obtained from Sigma-Aldrich Co. LLC.…”

Section: Hemicellulose Monosaccharide Determination By Gc-msmentioning

confidence: 99%

See 2 more Smart Citations

Three Fiber Crops Show Distinctive Biomass Saccharification under Physical and Chemical Pretreatments by Altered Wall Polymer Features

Wei¹,

Zhou²,

Huang³

et al. 2016

BioResources

Self Cite

View full text Add to dashboard Cite

Jute, kenaf, and ramie are important fiber crops in the textile industry, but their core stalks have not been well used. In this study, three fiber crops were examined with enhanced hexoses yields (% cellulose) up to 4.8-, 3.6-, and 1.9-fold from enzymatic hydrolysis of the steam-exploded stalks. Sequential dilute acid/alkali pretreatments achieved the increased hexoses yields up to 7.6-, 4.8-, and 2.8-fold. Without steam explosion, the three crops could increase hexoses yields under extremely high concentrations of NaOH (12% to 16%). While kenaf and ramie mainly showed hemicelluloses removal with steam explosion, jute exhibited an effective co-extraction of hemicelluloses and lignin, which greatly reduced its cellulose DP, hemicellulosic Xyl/Ara, and G-monomer. These findings explain why jute had the highest hexoses yield among the three crops and also suggest that G-monomer and Xyl may interact in a way that mainly determines wall polymers co-extraction with steam explosion.

show abstract

Section: Extraction Of Wall Polymers With Steam Explosionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Section: Hemicellulose Monosaccharide Determination By Gc-msmentioning

confidence: 99%

See 1 more Smart Citation

Three Fiber Crops Show Distinctive Biomass Saccharification under Physical and Chemical Pretreatments by Altered Wall Polymer Features

Wei¹,

Zhou²,

Huang³

et al. 2016

BioResources

Self Cite

View full text Add to dashboard Cite

show abstract

“…Hemicelluloses play an important role in biomass enzymatic digestion (Xu et al 2012;Zhang et al 2012;Li et al 2013a). Xylan, the major hemicellulosic polysaccharide present in plant cell walls, has a backbone of β-1,4-linked xylose residues and side chains of different substituents (Vázquez et al 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Miscanthus with high biomass yield and low nitrogen and water requirement is considered as one of leading feedstocks for biofuel and value-added chemicals production (Xu et al 2012;Li et al 2013a). The aim of this work was to investigate the application potential of xylan-degrading enzymes from A. niger BE-2 and Hypocrea orientalis EU7-22 for the bioconversion of Miscanthus into monosaccharides and XOS.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Enzymatic Hydrolysis of Miscanthus Xylan using Aspergillus niger, Hypocrea orientalis, and Trichoderma reesei Xylan-degrading Enzymes

Liu

et al. 2014

BioRes

View full text Add to dashboard Cite

Xylan-degrading enzymes from Aspergillus niger and Hypocrea orientalis were characterized by enzyme activity assays and protein profiling with SDS-PAGE and LC-MS/MS. The hydrolysis of Miscanthus xylan by xylan-degrading enzymes from A. niger, H. orientalis, and Trichoderma reesei were comparatively studied by HPLC analysis. It was found that the glycoside hydrolase families 10 xylanase was the main xylanase secreted by H. orientalis and A. niger when using corn cob and wheat bran as inducers. Compared to the enzymes from T. reesei,the enzymes from A. niger showed better efficiency in the hydrolysis of Miscanthus xylan into monosaccharides. Nevertheless, the enzymes from H. orientalis were more preferable for the hydrolysis of Miscanthus xylan into xylo-oligosaccharides (XOS), especially xylobiose and xylotriose. Miscanthus xylan degradation was significantly influenced by the activities of β-xylosidase and α-L-arabinofuranosidase. Xylan-degrading enzymes with high ratios of β-xylosidase and α-L-arabinofuranosidase are necessary for the efficient conversion of Miscanthus xylan into monosaccharides. However, xylan-degrading enzymes with low β-xylosidase activity and high α-L-arabinofuranosidase activity were required for producing XOS.

show abstract

Dielectric barrier discharge plasma microbubble reactor for pretreatment of lignocellulosic biomass

et al. 2018

View full text Add to dashboard Cite

A novel lignocellulosic biomass pretreatment reactor has been designed and tested to investigate pretreatment efficacy of miscanthus grass. The reactor was designed to optimize the transfer of highly oxidative species produced by dielectric barrier discharge plasma to the liquid phase immediately after generation, by arranging close proximity of the plasma to the gas-liquid interface of microbubbles. The reactor produced a range of reactive oxygen species and reactive nitrogen species, and the rate of production depended on the power source duty cycle and the temperature of the plasma. Ozone and other oxidative species were dispersed efficiently using energy efficient microbubbles produced by fluidic oscillations. A 5% (w/w) miscanthus suspension pretreated for 3 h at 10% duty cycle yielded 0.5% acid soluble lignin release and 26% sugar release post hydrolysis with accelerated pretreatment toward the latter stages of the treatment demonstrating the potential of this approach as an alternative pretreatment method.

show abstract

Arabinose substitution degree in xylan positively affects lignocellulose enzymatic digestibility after various NaOH/H2SO4 pretreatments in Miscanthus

Cited by 117 publications

References 35 publications

Three Fiber Crops Show Distinctive Biomass Saccharification under Physical and Chemical Pretreatments by Altered Wall Polymer Features

Three Fiber Crops Show Distinctive Biomass Saccharification under Physical and Chemical Pretreatments by Altered Wall Polymer Features

Comparative Analysis of Enzymatic Hydrolysis of Miscanthus Xylan using Aspergillus niger, Hypocrea orientalis, and Trichoderma reesei Xylan-degrading Enzymes

Dielectric barrier discharge plasma microbubble reactor for pretreatment of lignocellulosic biomass

Contact Info

Product

Resources

About