Arabinogalactan:β-glucan as novel biodegradable carriers for recombinant human thrombin

Abstract

The aim of this work was to evaluate the effects of incorporating thrombin in arabinogalactan (AG)/β-glucan (BG)-based carriers. The products were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction (XRPD) and X-ray photoelectron spectroscopy techniques. Results, especially deconvoluted XRPD patterns indicated creation of new phases and potential complex formation. Results also highlighted that the AG carrier leads to higher residual thrombin-specific activity, while the in vivo haemostatic effect was enhanced when insoluble BG was present in the matrix. Our results confirm that thrombin can be successfully added to the carriers and that these materials are promising alternatives to standard vehicles.

Thrombin‐Loaded Poly(butylene succinate)‐Based Electrospun Membranes for Rapid Hemostatic Application

“…Many substances such as arabinogalactan and gelatin have stabilizing effect on thrombin . Prior to immobilization of thrombin onto PBS membrane, the effect of gelatin on stabilization of thrombin solution was investigated in order to evaluate the necessity to include gelatin into the membrane.…”

“…Immobilizing thrombin onto support materials can not only facilitate its application by avoiding pre‐application preparation but also increase its storage stability compared to thrombin solution, and therefore rapid hemostatic materials with easy handling, direct application, and longer shelf life could be prepared by good choice of support materials (carriers) coupled with enzymatic activity of thrombin. Some successful work has been reported . For example, Baker et al have immobilized thrombin onto mesocellular silicate foams, and the thrombin‐loaded foam shows shorter blood clotting time in vitro than the corresponding neat foam and QuickClot, a commercial hemostatic material.…”

Optimizing the synthesis of yeast Beta-glucan via response surface methodology for nanotechnology application

“…The absorbance of aliphatic CH x bands present in the range 2800–3000 cm −1 and the intensity of vibration of CH 3 is greater than aliphatic CH 2 or CH stretching vibration [ 39 ]. The band observed at 3300.17 cm −1 reflects the stretching of the hydroxyl group (O–H) that shares a hydrogen bond formation; the main cause of the helix structure of β-glucan [ 40 ]. As shown in Fig.…”

Bioactive Hydrogels with Pro‐coagulation Effect for Hemostasis†

“…[ 111 ] Thrombin can be loaded into hemostatic materials by adsorption of positive molecules such as chitosan and mussel‐like structures such as gallic acid and polydopamine. [ 112‐113 ] Whether natural polypeptides, polysaccharides, [ 114‐118 ] or synthetic fiber materials, [ 119‐120 ] thrombin can be easily loaded in. For example, mixing thrombin into electrospinning fiber precursors can easily obtain the hemostatic material.…”