Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments

Pfeifer, Lukas; Shafee, Thomas; Johnson, Kim L.; Bacic, Antony; Classen, Birgit

doi:10.1038/s41598-020-65135-5

Cited by 44 publications

(68 citation statements)

References 71 publications

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“…In the field of wall proteins, the recent findings from our group (Pfeifer et al, 2020) could underline the hypothesis of an involvement of highly charged AGPs in calcium storage andsignaling (Lamport et al, 2006;Lamport et al, 2020). To answer the question whether AGPs are present in other seagrass species and whether they are structurally comparable to Zostera AGP is another challenge for the future.…”

Section: Conclusion With Regard To Existing Workmentioning

confidence: 86%

“…These assumptions were based on monosaccharide and amino acid composition of acidic wall preparations. Pfeifer et al (2020) performed the first isolation and structural characterization of a βGlcY-precipitable AGP from Z. marina (Table 2). Here whole plants, rhizomes, roots and leaves were extracted and investigated.…”

Section: Wall (Glyco)proteinsmentioning

confidence: 99%

“…The content of cellulose, hemicelluloses and lignin is often estimated by simple procedures using different hydrolytic methods and weighing of the residual material. On the other hand, some detailed investigations of single seagrass polysaccharides in single species shed light on some aspects of seagrass cell wall adaption to the marine habitat (e.g., Aquino et al, 2005;Gloaguen et al, 2010;Lv et al, 2015;Pfeifer et al, 2020). All together, similarities and differences between the different seagrass families and species and also the comparison to cell walls of monocotyledonous land plants are still a mystery.…”

Section: Conclusion With Regard To Existing Workmentioning

confidence: 99%

“…Furthermore, it has to be investigated whether the interesting unique polysaccharides/glycoproteins which have been isolated and carefully characterized for single seagrass species are also present in other or even all seagrasses. Especially the apiogalacturonans (Gloaguen et al, 2010;Lv et al, 2015), sulfated galactans (Aquino et al, 2005) and highly charged AGPs (Pfeifer et al, 2020) are interesting candidates for adaption to the marine habitat. Comparable to marine algae, charged polysaccharides/glycoproteins of the cell wall seem to be essential to cope with salt stress.…”

Section: Main Challenges For the Futurementioning

confidence: 99%

“…Distinct glycan modifications have been identified in different species and tissues and are suggested to influence both their physical properties and function. Recently, AGPs have been isolated and structurally characterized for the first time from a seagrass (Pfeifer et al, 2020). Although the common backbone structure of land plant AGPs is conserved, the glycan structures exhibit unique features, including a high degree of branching and an unusually high content of terminating 4-O-methyl-glucuronic acid (4-OMe GlcA) residues, suggesting a role of seagrass AGPs in osmoregulation (Lamport et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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The Cell Wall of Seagrasses: Fascinating, Peculiar and a Blank Canvas for Future Research

Pfeifer

Classen

2020

Front. Plant Sci.

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Seagrasses are a polyphyletic group of angiosperm plants, which evolved from early monocotyledonous land plants and returned to the marine environment around 140 million years ago. Today, seagrasses comprise the five families Zosteraceae, Hydrocharitaceae, Posidoniaceae, Cymodoceaceae, and Ruppiaceae and form important coastal ecosystems worldwide. Despite of this ecological importance, the existing literature on adaption of these angiosperms to the marine environment and especially their cell wall composition is limited up to now. A unique feature described for some seagrasses is the occurrence of polyanionic, low-methylated pectins mainly composed of galacturonic acid and apiose (apiogalacturonans). Furthermore, sulfated galactans have been detected in some species. Recently, arabinogalactan-proteins (AGPs), highly glycosylated proteins of the cell wall of land plants, have been isolated for the first time from a seagrass of the baltic sea. Obviously, seagrass cell walls are characterized by new combinations of structural polysaccharide and glycoprotein elements known from macroalgae and angiosperm land plants. In this review, current knowledge on cell walls of seagrasses is summarized and suggestions for future investigations are given.

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Section: Conclusion With Regard To Existing Workmentioning

confidence: 86%

Section: Wall (Glyco)proteinsmentioning

confidence: 99%

Section: Conclusion With Regard To Existing Workmentioning

confidence: 99%

Section: Main Challenges For the Futurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Cell Wall of Seagrasses: Fascinating, Peculiar and a Blank Canvas for Future Research

Pfeifer

Classen

2020

Front. Plant Sci.

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Enhancing the fire resistance properties of thermally modified Robinia pseudoacacia wood with natural and synthetic flame retardants: chemical characterisation and fire behaviour

Marino,

Gaff,

Sethy

et al. 2024

Eur. J. Wood Prod.

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The increasing demand for wood with enhanced flame retardant characteristics in construction applications necessitates strategic interventions. This study explores the fire behaviour and chemical characterisation of Robinia pseudoacacia wood subjected to thermal modification and flame retardant treatments. Thermal modification was carried out at three different temperatures (160 °C, 180 °C and 240 °C). The fire properties of wood coated with Flame Gard (F), a commercial flame retardant, arabinogalactan (A), a natural flame retardant, melamine adhesive (MF) with ammonium polyphosphate (AP), nanosilica (NS), nanoclay (NC) (MF-AP-NS and MF-AP-NC) and arabinogalactan with AP, NS and NC (A-AP-NS and A-AP-NC), were assessed using cone calorimetry in terms of the weight loss rate, ignition time and heat release rate. The commercial flame retardant Flame Gard outperformed the natural and fortified flame retardants in terms of the weight loss rate, heat release rate (HRR) and ignition time (tig). Unmodified samples exhibited superior fire properties in terms of tig and HRR compared to thermally modified samples. The peak heat release rate (kW.m− 2) and time to peak heat release rate (s) showed a moderate degree of dependency on the chemical constituents of the wood.

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Different responses of banana classical AGP genes and cell wall AGP components to low-temperature between chilling sensitive and tolerant cultivars

et al. 2022

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Classical arabinogalactan proteins (AGPs) belong to glycosylphosphatidylinositol-anchored proteins, which are proved to be involved in signaling and cell wall metabolism upon stresses. However, rare information is available on the roles of classical AGPs in low temperature (LT) tolerance. Cultivation of banana in tropical and subtropical region is seriously threatened by LT stress. In the present study, 17 classical MaAGPs and nine MbAGPs in banana A and B genome were identi ed and characterized, respectively. Great diversity was present among different classical MaAGP/MbAGP members while ve members (AGP3/6/11/13/14) showed 100% identity between these two gene families. We further investigated different responses of classical AGPs to LT between a chilling sensitive (CS) and tolerant (CT) banana cultivars. In addition, different changes in the temporal and spatial distribution of cell wall AGP components under LTs between these two cultivars were compared using immuno uorescence labeling. Seven classical MbAGPs were upregulated by LT(s) in the CT cultivar. Classical MaAGP4/6 was induced by LT(s) in both cultivars while MaAGP1/2/9/16/17 only in the CT cultivar. Moreover, these genes showed signi cantly higher transcription abundance in the CT cultivar than the CS one under LT(s) except classical MaAGP4. Similar results were observed with the epitopes of JIM13 and LM2 antibodies. The antigens of these antibodies and classical MaAGP1/2/6/9/16/17 might be related to LT tolerance of banana. These results provide additional information about plant classical AGPs and their involvement in LT tolerance, as well as their potential as candidate genes to be targeted when breeding CT banana. Key MessageSeven-teen classical MaAGPs and nine MbAGPs were identi ed and analyzed. MaAGP1/2/6/9/16/17, the antigens of JIM13 and LM2 antiboides are likely to be involved in banana chilling tolerance.

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Arabinogalactan-proteins of Zostera marina L. contain unique glycan structures and provide insight into adaption processes to saline environments

Cited by 44 publications

References 71 publications

The Cell Wall of Seagrasses: Fascinating, Peculiar and a Blank Canvas for Future Research

The Cell Wall of Seagrasses: Fascinating, Peculiar and a Blank Canvas for Future Research

Enhancing the fire resistance properties of thermally modified Robinia pseudoacacia wood with natural and synthetic flame retardants: chemical characterisation and fire behaviour

Different responses of banana classical AGP genes and cell wall AGP components to low-temperature between chilling sensitive and tolerant cultivars

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