Arabinogalactan glycoprotein dynamics during the progamic phase in the tomato pistil

Lara-Mondragón, Cecilia Monserrat; MacAlister, Cora A.

doi:10.1007/s00497-021-00408-1

Cited by 11 publications

(9 citation statements)

References 113 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For tomato, we found that the pollen and PT samples were heavily enriched for expression of SlSEC1A , while the other SM genes had depleted expression in the pollen samples. In our original analysis of the transcriptomic data, we had identified the second (larger) tomato SEC1A model as one of the 1229 tomato genes with enriched expression in pollen grain samples (Lara‐Mondragón & MacAlister, 2021).…”

Section: Resultsmentioning

confidence: 99%

“…To explore the possible role of SEC1 proteins as a general feature of PT growth, we identified SM family members in species for which pollen expression data were available. We have recently published a transcriptomic data set for tomato ( Solanum lycopersicum ) reproductive tissues including pollen and PTs (Lara‐Mondragón & MacAlister, 2021). Transcriptomic data were also available for rice, maize and the basal angiosperm Amborella trichopoda (Wei et al., 2010; Davidson et al., 2011; Flores‐Tornero et al., 2020).…”

Section: Resultsmentioning

confidence: 99%

“…(b–e) Heatmaps corresponding to the log2 of the mean expression values for the indicated genes based on available mRNA‐seq or microarray data. (b) Tomato expression values (Lara‐Mondragón & MacAlister, 2021). (c) Maize expression values (Davidson et al., 2011).…”

Section: Resultsmentioning

confidence: 99%

“…Expression values of the SM homologs were gathered from published RNA‐seq data (tomato – Lara‐Mondragón & MacAlister, 2021; maize – Davidson et al., 2011; Amborella – Flores‐Tornero et al., 2020; Arabidopsis – Loraine et al., 2013) or microarray data (rice – Wei et al., 2010; Arabidopsis –Klepikova et al., 2016), and a heatmap corresponding to the log2 of the mean expression values per gene was plotted using the pheatmap package in Rstudio. Species icons were generated using BioRender.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

SEC1A is a major Arabidopsis Sec1/Munc18 gene in vesicle trafficking during pollen tube tip growth

et al. 2022

Self Cite

View full text Add to dashboard Cite

SUMMARY Pollen tubes (PTs) grow by the targeted secretion of new cell wall material to their expanding tip region. Sec1/Munc18 (SM) proteins promote membrane fusion through regulation of the SNARE complex. We have previously shown that disruption of protein glycosylation in the Arabidopsis thaliana hpat1 hpat3 double mutant leads to PT growth defects that can be suppressed by reducing secretion. Here, we identified five point mutant alleles of the SM protein SEC1A as hpat1/3 suppressors. The suppressors increased seed set, reduced PT growth defects and reduced the rate of glycoprotein secretion. In the absence of the hpat mutations, sec1a reduced pollen germination and PT elongation producing shorter and wider PTs. Consistent with a defect in membrane fusion, sec1a PTs accumulated secretory vesicles. Though sec1a had significantly reduced male transmission, homozygous sec1a plants maintained full seed set, demonstrating that SEC1A was ultimately dispensable for pollen fertility. However, when combined with a mutation in another SEC1‐like SM gene, keule, pollen fertility was totally abolished. Mutation in sec1b, the final member of the Arabidopsis SEC1 clade, did not enhance the sec1a phenotype. Thus, SEC1A is the major SM protein promoting pollen germination and tube elongation, but in its absence KEULE can partially supply this activity. When we examined the expression of the SM protein family in other species for which pollen expression data were available, we found that at least one Sec1‐like protein was highly expressed in pollen samples, suggesting a conserved role in pollen fertility in other species.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

SEC1A is a major Arabidopsis Sec1/Munc18 gene in vesicle trafficking during pollen tube tip growth

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, it was shown that cell wall O-glycans (i.e., AGPs) play a crucial role during pistil development in tomato flowers during the progamic phase. It was concluded that AGP accumulation correlates with stigma maturation [ 176 ]. A comprehensive overview of the role of O-glycans for plant development, and floral development in particular is given by Seifert [ 24 ].…”

Section: Developmental Consequences Of Glycosylation: From Flowers To Germinating Seedsmentioning

confidence: 99%

Sweet Modifications Modulate Plant Development

et al. 2021

View full text Add to dashboard Cite

Plant development represents a continuous process in which the plant undergoes morphological, (epi)genetic and metabolic changes. Starting from pollination, seed maturation and germination, the plant continues to grow and develops specialized organs to survive, thrive and generate offspring. The development of plants and the interplay with its environment are highly linked to glycosylation of proteins and lipids as well as metabolism and signaling of sugars. Although the involvement of these protein modifications and sugars is well-studied, there is still a long road ahead to profoundly comprehend their nature, significance, importance for plant development and the interplay with stress responses. This review, approached from the plants’ perspective, aims to focus on some key findings highlighting the importance of glycosylation and sugar signaling for plant development.

show abstract

JAGGER localization and function are dependent on GPI anchor addition

Figueiredo,

Costa,

Moreira

et al. 2024

Plant Reprod

View full text Add to dashboard Cite

Key message GPI anchor addition is important for JAGGER localization and in vivo function. Loss of correct GPI anchor addition in JAGGER, negatively affects its localization and function. Abstract In flowering plants, successful double fertilization requires the correct delivery of two sperm cells to the female gametophyte inside the ovule. The delivery of a single pair of sperm cells is achieved by the entrance of a single pollen tube into one female gametophyte. To prevent polyspermy, Arabidopsis ovules avoid the attraction of multiple pollen tubes to one ovule–polytubey block. In Arabidopsisjagger mutants, a significant number of ovules attract more than one pollen tube to an ovule due to an impairment in synergid degeneration. JAGGER encodes a putative arabinogalactan protein which is predicted to be anchored to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. Here, we show that JAGGER fused to citrine yellow fluorescent protein (JAGGER-cYFP) is functional and localizes mostly to the periphery of ovule integuments and transmitting tract cells. We further investigated the importance of GPI-anchor addition domains for JAGGER localization and function. Different JAGGER proteins with deletions in predicted ω-site regions and GPI attachment signal domain, expected to compromise the addition of the GPI anchor, led to disruption of JAGGER localization in the cell periphery. All JAGGER proteins with disrupted localization were also not able to rescue the polytubey phenotype, pointing to the importance of GPI-anchor addition to in vivo function of the JAGGER protein.

show abstract

Arabinogalactan glycoprotein dynamics during the progamic phase in the tomato pistil

Cited by 11 publications

References 113 publications

SEC1A is a major Arabidopsis Sec1/Munc18 gene in vesicle trafficking during pollen tube tip growth

SEC1A is a major Arabidopsis Sec1/Munc18 gene in vesicle trafficking during pollen tube tip growth

Sweet Modifications Modulate Plant Development

JAGGER localization and function are dependent on GPI anchor addition

Contact Info

Product

Resources

About