Arabidopsis Voltage-Dependent Anion Channel 1 (AtVDAC1) Is Required for Female Development and Maintenance of Mitochondrial Functions Related to Energy-Transaction

Pan, Xiaodi; Chen, Ziwei; Yang, Xue; Liu, Guoqin

doi:10.1371/journal.pone.0106941

Cited by 17 publications

(15 citation statements)

References 69 publications

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“…Last, O 2 uptake by the OXPHOS pathway was reduced in vdac1, and also probably in vdac3. Reduced ATP synthesis and reduced membrane potential have already been observed in vdac1 mutants [4,40], and reduced membrane potential has been found in vdac3, but also in vdac2 and vdac4 KO lines [4]. VDAC3 overexpression did not seem able to restore OXPHOS respiration in vdac1_OEV3, suggesting that VDAC1 and VDAC3 have some specialized functions that do not overlap.…”

Section: Discussionmentioning

confidence: 88%

Arabidopsis Voltage-Dependent Anion Channels (VDACs): Overlapping and Specific Functions in Mitochondria

Hémono

Ubrig

Azeredo

et al. 2020

Cells

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Voltage-dependent anion channels (VDACs) are essential components of the mitochondrial outer membrane. VDACs are involved in the exchange of numerous ions and molecules, from ATP to larger molecules such as tRNAs, and are supposed to adjust exchanges in response to cell signals and stresses. Four major VDACs have been identified in Arabidopsis thaliana. The goal of this study was to explore the specific functions of these proteins, in particular, in tRNA import into mitochondria and stress response. The main results were: (i) VDACs appeared to differentially interact with tRNAs, and VDAC4 could be the major tRNA channel on the outer membrane, (ii) a VDAC3 mRNA isoform was found induced by different stresses, suggesting that VDAC3 might be specifically involved in early steps of stress response and (iii) an analysis of vdac3 and vdac1 mutant lines showed that VDAC3 and VDAC1 shared some, but not all functions. In conclusion, this work brings new knowledge on VDACs, which do not appear as interchangeable pores of the outer membrane and each VDAC has its own specificity.

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Section: Discussionmentioning

confidence: 88%

Arabidopsis Voltage-Dependent Anion Channels (VDACs): Overlapping and Specific Functions in Mitochondria

Hémono

Ubrig

Azeredo

et al. 2020

Cells

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“…seedlings. In Arabidopsis, VDAC1 has a ubiquitous expression pattern but shows particularly high expression levels in reproductive organs and during germination and early seedling establishment (Pan et al, 2014).…”

Section: Matr Expression Profiles In Arabidopsismentioning

confidence: 99%

“…The levels of VDAC1 were also higher in germinating seeds compared mature (i.e., 3-week-old) seedlings. In Arabidopsis, VDAC1 is required for maintaining the steady state of mitochondrial transmembrane potential (DC) and hence for optimal respiratory activities, and its functions were found to be critical during the reproductive stage and during early germination (Pan et al, 2014). In contrast to MatR and Porin, the steady-state levels of various other mitochondrial proteins, including Nad9, CA2, RISP, COX2, and AtpB, all increased in during plant development, with the highest accumulation levels seen in mature plants.…”

Section: The Expression Of Matr Is Developmentally Regulatedmentioning

confidence: 99%

The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria

et al. 2016

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Group II introns are large catalytic RNAs that are ancestrally related to nuclear spliceosomal introns. Sequences corresponding to group II RNAs are found in many prokaryotes and are particularly prevalent within plants organellar genomes. Proteins encoded within the introns themselves (maturases) facilitate the splicing of their own host pre-RNAs. Mitochondrial introns in plants have diverged considerably in sequence and have lost their maturases. In angiosperms, only a single maturase has been retained in the mitochondrial DNA: the matR gene found within NADH dehydrogenase 1 (nad1) intron 4. Its conservation across land plants and RNA editing events, which restore conserved amino acids, indicates that matR encodes a functional protein. However, the biological role of MatR remains unclear. Here, we performed an in vivo investigation of the roles of MatR in Brassicaceae. Directed knockdown of matR expression via synthetically designed ribozymes altered the processing of various introns, including nad1 i4. Pull-down experiments further indicated that MatR is associated with nad1 i4 and several other intron-containing pre-mRNAs. MatR may thus represent an intermediate link in the gradual evolutionary transition from the intron-specific maturases in bacteria into their versatile spliceosomal descendants in the nucleus. The similarity between maturases and the core spliceosomal Prp8 protein further supports this intriguing theory.

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“…therein), and it is important to note that a functional interplay between these transport proteins is essential for mitochondrial activity and energy supply for the cell. Interestingly, in mammals, VDACs have been associated with PCD (Pan et al, 2014), while in plants, their contribution to cell death mechanisms remains elusive (Robert et al, 2012;Godbole et al, 2013).…”

Section: Isoforms Of Mitochondrial Transporters Are Differentially Exmentioning

confidence: 99%

Dissecting the Metabolic Role of Mitochondria during Developmental Leaf Senescence

Chrobok

Law²,

Brouwer³

et al. 2016

Plant Physiol.

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The functions of mitochondria during leaf senescence, a type of programmed cell death aimed at the massive retrieval of nutrients from the senescing organ to the rest of the plant, remain elusive. Here, combining experimental and analytical approaches, we showed that mitochondrial integrity in Arabidopsis (Arabidopsis thaliana) is conserved until the latest stages of leaf senescence, while their number drops by 30%. Adenylate phosphorylation state assays and mitochondrial respiratory measurements indicated that the leaf energy status also is maintained during this time period. Furthermore, after establishing a curated list of genes coding for products targeted to mitochondria, we analyzed in isolation their transcript profiles, focusing on several key mitochondrial functions, such as the tricarboxylic acid cycle, mitochondrial electron transfer chain, iron-sulfur cluster biosynthesis, transporters, as well as catabolic pathways. In tandem with a metabolomic approach, our data indicated that mitochondrial metabolism was reorganized to support the selective catabolism of both amino acids and fatty acids. Such adjustments would ensure the replenishment of α-ketoglutarate and glutamate, which provide the carbon backbones for nitrogen remobilization. Glutamate, being the substrate of the strongly up-regulated cytosolic glutamine synthase, is likely to become a metabolically limiting factor in the latest stages of developmental leaf senescence. Finally, an evolutionary age analysis revealed that, while branched-chain amino acid and proline catabolism are very old mitochondrial functions particularly enriched at the latest stages of leaf senescence, auxin metabolism appears to be rather newly acquired. In summation, our work shows that, during developmental leaf senescence, mitochondria orchestrate catabolic processes by becoming increasingly central energy and metabolic hubs.

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Arabidopsis Voltage-Dependent Anion Channel 1 (AtVDAC1) Is Required for Female Development and Maintenance of Mitochondrial Functions Related to Energy-Transaction

Cited by 17 publications

References 69 publications

Arabidopsis Voltage-Dependent Anion Channels (VDACs): Overlapping and Specific Functions in Mitochondria

Arabidopsis Voltage-Dependent Anion Channels (VDACs): Overlapping and Specific Functions in Mitochondria

The Reverse Transcriptase/RNA Maturase Protein MatR Is Required for the Splicing of Various Group II Introns in Brassicaceae Mitochondria

Dissecting the Metabolic Role of Mitochondria during Developmental Leaf Senescence

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