Arabidopsis thaliana TBP-associated factor 5 is essential for plant growth and development

Mougiou, Niki; Poulios, Stylianos; Kaldis, Athanasios; Vlachonasios, Konstantinos E.

doi:10.1007/s11032-011-9626-2

Cited by 16 publications

(13 citation statements)

References 64 publications

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“…Of the 74 downregulated genes, a few of transcriptional regulatory genes which have been demonstrated to have important roles in plant growth and development were identified, including the phytochrome interacting factor 4 (Kunihiro et al ), the TATA binding protein‐associated factor 5 (Mougiou et al ), a BRX family gene (Briggs et al ), and the cyclin B2:1 (Weingartner et al ). In addition, three genes functioning in histone modification or chromatin remodeling were found to be downregulated, including the E3 ubiquitin‐protein ligase RING1a (Endoh et al ), a histone acetyltransferase of the GNAT family 2, and the SWI/SNF complex subunit SWI3D (Carrozza et al ; Jerzmanowski ).…”

Section: Resultsmentioning

confidence: 99%

Overexpression of the maize GRF10, an endogenous truncated growth‐regulating factor protein, leads to reduction in leaf size and plant height

Zhang

Xue

et al. 2014

JIPB

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It has long been thought that growth-regulating factors (GRFs) gene family members act as transcriptional activators to play important roles in multiple plant developmental processes. However, the recent characterization of Arabidopsis GRF7 showed that it functions as a transcriptional repressor of osmotic stress-responsive genes. This highlights the complex and diverse mechanisms by which different GRF members use to take action. In this study, the maize (Zea mays L.) GRF10 was functionally characterized to improve this concept. The deduced ZmGRF10 protein retains the N-terminal QLQ and WRC domains, the characteristic regions as protein-interacting and DNA-binding domains, respectively. However, it lacks nearly the entire C-terminal domain, the regions executing transactivation activity. Consistently, ZmGRF10 protein maintains the ability to interact with GRF-interacting factors (GIFs) proteins, but lacks transactivation activity. Overexpression of ZmGRF10 in maize led to a reduction in leaf size and plant height through decreasing cell proliferation, whereas the yield-related traits were not affected. Transcriptome analysis revealed that multiple biological pathways were affected by ZmGRF10 overexpression, including a few transcriptional regulatory genes, which have been demonstrated to have important roles in controlling plant growth and development. We propose that ZmGRF10 aids in fine-tuning the homeostasis of the GRF-GIF complex in the regulation of cell proliferation.

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Section: Resultsmentioning

confidence: 99%

Overexpression of the maize GRF10, an endogenous truncated growth‐regulating factor protein, leads to reduction in leaf size and plant height

Zhang

Xue

et al. 2014

JIPB

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“…; Mougiou et al . ). FBH3 and its homologs are the transcriptional activators of CONSTANS (CO), which is the photoperiodic flowering regulator (Ito et al .…”

Section: Discussionmentioning

confidence: 97%

“…FBH3 and TAF5 are likely to be involved in developmental processes (Ito et al 2012;Mougiou et al 2012). FBH3 and its homologs are the transcriptional activators of CONSTANS (CO),which is the photoperiodic flowering regulator (Ito et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Four Arabidopsis AREB/ABF transcription factors function predominantly in gene expression downstream of SnRK2 kinases in abscisic acid signalling in response to osmotic stress

Yoshida

Fujita

Maruyama

et al. 2014

Plant Cell & Environment

485

430

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Under osmotic stress conditions such as drought and high salinity, the plant hormone abscisic acid (ABA) plays important roles in stress-responsive gene expression mainly through three bZIP transcription factors, AREB1/ABF2, AREB2/ABF4 and ABF3, which are activated by SNF1-related kinase 2s (SnRK2s) such as SRK2D/SnRK2.2, SRK2E/SnRK2.6 and SRK2I/SnRK2.3 (SRK2D/E/I). However, since the three AREB/ABFs are crucial, but not exclusive, for the SnRK2-mediated gene expression, transcriptional pathways governed by SRK2D/E/I are not fully understood. Here, we show that a bZIP transcription factor, ABF1, is a functional homolog of AREB1, AREB2 and ABF3 in ABA-dependent gene expression in Arabidopsis. Despite lower expression levels of ABF1 than those of the three AREB/ABFs, the areb1 areb2 abf3 abf1 mutant plants displayed increased sensitivity to drought and decreased sensitivity to ABA in primary root growth compared with the areb1 areb2 abf3 mutant. Genome-wide transcriptome analyses revealed that expression of downstream genes of SRK2D/E/I, which include many genes functioning in osmotic stress responses and tolerance such as transcription factors and LEA proteins, was mostly impaired in the quadruple mutant. Thus, these results indicate that the four AREB/ABFs are the predominant transcription factors downstream of SRK2D/E/I in ABA signalling in response to osmotic stress during vegetative growth.Abscisic acid (ABA) plays important roles in osmotic stress-responsive gene expression mainly through three bZIP transcription factors, AREB1, AREB2, and ABF3, which are activated by SnRK2s such as SRK2D, SRK2E, and SRK2I (SRK2D/E/I). However, transcription factors other than the three AREB/ABFs that function downstream of SRK2D/E/I remain obscure. Here, we report that ABF1 is a functional homolog of AREB1, AREB2, and ABF3 in ABA-dependent gene expression from a comparative analysis between the areb1 areb2 abf3 abf1 and areb1 areb2 abf3 mutants. Moreover, genome-wide transcriptome analyses revealed that expression of downstream genes of SRK2D/E/I were mostly impaired in the areb1 areb2 abf3 abf1 quadruple mutant, suggesting that the four AREB/ABFs are the predominant transcription factors downstream of SRK2D/E/I in ABA signaling in response to osmotic stress.

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“…Arabidopsis sgf29a⁻ shows a little delay in leaf and flower development [ 61 ]. Importantly, some reports on plant TAFs (TAF5, 6 and 10) indicated their indispensable role in plant development [ 81 , 83 , 90 ]. Notably, SAGA complex is also critically involved in developmental aspects and is indispensable for viability in metazoan [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

Spt-Ada-Gcn5-Acetyltransferase (SAGA) Complex in Plants: Genome Wide Identification, Evolutionary Conservation and Functional Determination

et al. 2015

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The recruitment of RNA polymerase II on a promoter is assisted by the assembly of basal transcriptional machinery in eukaryotes. The Spt-Ada-Gcn5-Acetyltransferase (SAGA) complex plays an important role in transcription regulation in eukaryotes. However, even in the advent of genome sequencing of various plants, SAGA complex has been poorly defined for their components and roles in plant development and physiological functions. Computational analysis of Arabidopsis thaliana and Oryza sativa genomes for SAGA complex resulted in the identification of 17 to 18 potential candidates for SAGA subunits. We have further classified the SAGA complex based on the conserved domains. Phylogenetic analysis revealed that the SAGA complex proteins are evolutionary conserved between plants, yeast and mammals. Functional annotation showed that they participate not only in chromatin remodeling and gene regulation, but also in different biological processes, which could be indirect and possibly mediated via the regulation of gene expression. The in silico expression analysis of the SAGA components in Arabidopsis and O. sativa clearly indicates that its components have a distinct expression profile at different developmental stages. The co-expression analysis of the SAGA components suggests that many of these subunits co-express at different developmental stages, during hormonal interaction and in response to stress conditions. Quantitative real-time PCR analysis of SAGA component genes further confirmed their expression in different plant tissues and stresses. The expression of representative salt, heat and light inducible genes were affected in mutant lines of SAGA subunits in Arabidopsis. Altogether, the present study reveals expedient evidences of involvement of the SAGA complex in plant gene regulation and stress responses.

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Arabidopsis thaliana TBP-associated factor 5 is essential for plant growth and development

Cited by 16 publications

References 64 publications

Overexpression of the maize GRF10, an endogenous truncated growth‐regulating factor protein, leads to reduction in leaf size and plant height

Overexpression of the maize GRF10, an endogenous truncated growth‐regulating factor protein, leads to reduction in leaf size and plant height

Four Arabidopsis AREB/ABF transcription factors function predominantly in gene expression downstream of SnRK2 kinases in abscisic acid signalling in response to osmotic stress

Spt-Ada-Gcn5-Acetyltransferase (SAGA) Complex in Plants: Genome Wide Identification, Evolutionary Conservation and Functional Determination

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