Arabidopsis thaliana Myb59 Gene Is Involved in the Response to Heterodera schachtii Infestation, and Its Overexpression Disturbs Regular Development of Nematode-Induced Syncytia

Wiśniewska, Anita; Wojszko, Kamila; Różańska, Elżbieta; Lenarczyk, Klaudia; Kuczerski, Karol; Sobczak, Mirosław

doi:10.3390/ijms22126450

Cited by 15 publications

(10 citation statements)

References 44 publications

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“…Therefore, according to the positions of the six GAMYB genes in the phylogenetic tree ( Supplementary Figure S3 ), a closer evolutionary relationship was observed with AtMYB33 ; therefore, they are renamed GmMYB33 a/b/c/d/e/f in our study ( Supplementary Figure S3 ). GAMYB genes were reported to function as immunity regulators in several plants such as rice [ 42 ] and Arabidopsis [ 49 ]. However, transcriptome analyses showed that soybean MYB genes expressed differently in both SCN resistance and susceptible cultivars [ 50 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

Soybean miR159-GmMYB33 Regulatory Network Involved in Gibberellin-Modulated Resistance to Heterodera glycines

Lei

Zhou

et al. 2021

IJMS

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Soybean cyst nematode (SCN, Heterodera glycines) is an obligate sedentary biotroph that poses major threats to soybean production globally. Recently, multiple miRNAome studies revealed that miRNAs participate in complicated soybean-SCN interactions by regulating their target genes. However, the functional roles of miRNA and target genes regulatory network are still poorly understood. In present study, we firstly investigated the expression patterns of miR159 and targeted GmMYB33 genes. The results showed miR159-3p downregulation during SCN infection; conversely, GmMYB33 genes upregulated. Furthermore, miR159 overexpressing and silencing soybean hairy roots exhibited strong resistance and susceptibility to H. glycines, respectively. In particular, miR159-GAMYB genes are reported to be involve in GA signaling and metabolism. Therefore, we then investigated the effects of GA application on the expression of miR159-GAMYB module and the development of H. glycines. We found that GA directly controls the miR159-GAMYB module, and exogenous GA application enhanced endogenous biologically active GA1 and GA3, the abundance of miR159, lowered the expression of GmMYB33 genes and delayed the development of H. glycines. Moreover, SCN infection also results in endogenous GA content decreased in soybean roots. In summary, the soybean miR159-GmMYB33 module was directly involved in the GA-modulated soybean resistance to H. glycines.

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Section: Discussionmentioning

confidence: 99%

Soybean miR159-GmMYB33 Regulatory Network Involved in Gibberellin-Modulated Resistance to Heterodera glycines

Lei

Zhou

et al. 2021

IJMS

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“… [ 18 ] C31(S13) AtMYB50, 55, 61 and 86 PtrMYB042, 055, 074, 121, 148, 170 and 216 MaMYB67 and 87 stomatal aperture [ 66 , 67 ] C32(S20) AtMYB2, 62, 78, 108, 112 and 116 PtrMYB034, 036, 066, 142, 164, 202 and 210 MaMYB40, 41, 74 and 95 response to fungal attack [ 61 , 68 , 69 ] C33(S19) AtMYB21, 24 and 57 PtrMYB204 and 207 MaMYB8 flower-specific transcription factor, is regulated by COP1 [ 70 , 71 ] C34(S18) AtMYB33, 65, 81, 97, 101, 104 and 120 PtrMYB007, 012, 024, 110, 124 and PtrMYB3R03 MaMYB2, 17 and 25 anther and pollen development. Response to ABA, anoxia and cold stress [ 1 ] C35 AtMYB71 and 79 PtrMYB014, 015,098, 195, 214 and 229 MaMYB12 stress response [ 72 ] C36 AtMYB27 PtrMYB103 MaMYB45 Phenylpropanoid pathway [ 73 ] C37 AtMYB48, 59, PtrMYB023 and 206 MaMYB69 Stress response [ 74 – 76 ] C38 AtMYB125 PtrMYB208 and 218 MaMYB84 male gametophyte development [ 17 ] C39(S25) AtMYB22, 98, 100, 115 and 118 PtrMYB001, 004, 022, 073, 147 and 151 MaMYB7, 68 and 88 Induction of green root and petal development [ 8 ] C40(S22) AtMYB44, 70, 73 and ...…”

Section: Resultsmentioning

confidence: 99%

Comprehensive analysis of the MYB transcription factor gene family in Morus alba

et al. 2022

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Background The V-myb myeloblastosis viral oncogene homolog (MYB) family of proteins is large, containing functionally diverse transcription factors. However, MYBs in Morus are still poorly annotated and a comprehensive functional analysis of these transcription factors is lacking. Results In the present study, a genome-wide identification of MYBs in Morus alba was performed. In total 166 MaMYBs were identified, including 103 R2R3-MYBs and four 3R-MaMYBs. Comprehensive analyses, including the phylogenetic analysis with putative functional annotation, motif and structure analysis, gene structure organization, promoter analysis, chromosomal localization, and syntenic relationships of R2R3-MaMYBs and 3R-MaMYBs, provided primary characterization for these MaMYBs. R2R3-MaMYBs covered the subgroups reported for R2R3-MYBs in Arabidopsis and Populus, and had two Morus-specific subgroups, indicating the high retention of MYBs in Morus. Motif analysis revealed high conservative residues at the start and end of each helix and residues consisting of the third helix in R2 and R3 repeats. Thirteen intron/exon patterns (a–m) were summarized, and the intron/exon pattern of two introns with phase numbers of 0 and 2 was the prevalent pattern for R2R3-MaMYBs. Various cis-elements in promoter regions were identified, and were mainly related to light response, development, phytohormone response, and abiotic and biotic stress response and secondary metabolite production. Expression patterns of R2R3-MaMYBs in different organs showed that MaMYBs involved in secondary cell wall components and stress responsiveness were preferentially expressed in roots or stems. R2R3-MaMYBs involved in flavonoid biosynthesis and anthocyanin accumulation were identified and characterized based on functional annotation and correlation of their expression levels with anthocyanin contents. Conclusion Based on a comprehensive analysis, this work provided functional annotation for R2R3-MYBs and an informative reference for further functional dissection of MYBs in Morus.

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“… Nishida et al (2017) found that two homologous genes, AtMYB48 and AtMYB59, show altered DNA-binding sites created by alternative splicing in response to environmental stresses of potassium deficiency. AtMYB59 is involved in the response to Heterodera schachtii infestation in A. thaliana ( Wisniewska et al, 2021 ). In addition, an R2R3 TF ZmMYB31 isolated from Zea mays can enhance superoxide dismutase and ascorbate peroxidase activities to protect plants against low temperature stress ( Li et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

The R2R3 Transcription Factor CsMYB59 Regulates Polyphenol Oxidase Gene CsPPO1 in Tea Plants (Camellia sinensis)

Huang

Qin

et al. 2021

Front. Plant Sci.

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Polyphenol oxidase (PPO) plays a role in stress response, secondary metabolism, and other physiological processes during plant growth and development, and is also a critical enzyme in black tea production. However, the regulatory mechanisms of PPO genes and their activity in tea plants are still unclear. In this study, we measured PPO activity in two different tea cultivars, Taoyuandaye (TYDY) and Bixiangzao (BXZ), which are commonly used to produce black tea and green tea, respectively. The expression pattern of CsPPO1 was assessed and validated via transcriptomics and quantitative polymerase chain reaction in both tea varieties. In addition, we isolated and identified an R2R3-MYB transcription factor CsMYB59 that may regulate CsPPO1 expression. CsMYB59 was found to be a nuclear protein, and its expression in tea leaves was positively correlated with CsPPO1 expression and PPO activity. Transcriptional activity analysis showed that CsMYB59 was a transcriptional activator, and the dual-luciferase assay indicated that CsMYB59 could activate the expression of CsPPO1 in tobacco leaves. In summary, our study demonstrates that CsMYB59 represents a transcriptional activator in tea plants and may mediate the regulation of PPO activity by activating CsPPO1 expression. These findings provide novel insights into the regulatory mechanism of PPO gene in Camellia sinensis, which might help to breed tea cultivars with high PPO activity.

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Arabidopsis thaliana Myb59 Gene Is Involved in the Response to Heterodera schachtii Infestation, and Its Overexpression Disturbs Regular Development of Nematode-Induced Syncytia

Cited by 15 publications

References 44 publications

Soybean miR159-GmMYB33 Regulatory Network Involved in Gibberellin-Modulated Resistance to Heterodera glycines

Soybean miR159-GmMYB33 Regulatory Network Involved in Gibberellin-Modulated Resistance to Heterodera glycines

Comprehensive analysis of the MYB transcription factor gene family in Morus alba

The R2R3 Transcription Factor CsMYB59 Regulates Polyphenol Oxidase Gene CsPPO1 in Tea Plants (Camellia sinensis)

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