Arabidopsis thaliana mutant that develops as a light-grown plant in the absence of light

Chory, Joanne; Peto, Charles A.; Feinbaum, Rhonda; Pratt, Lee H.; Ausubel, Frederick M.

doi:10.1016/0092-8674(89)90950-1

Cited by 458 publications

(461 citation statements)

References 26 publications

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“…Photomorphogenetic mutants such as deetiolatedl (def? ; Chory et al, 1989) and constitutivephotomorphogenid (copl; Deng et al, 1991) have high-level expression of CAB and RBCS in the dark, similar to light-grown wild-type plants. Because CUE1 is required for the expression of CAB and RBCS in the light, it is formally possible that the high-level expression of these genes in dark-grown detl and cop?…”

Section: Discussionmentioning

confidence: 99%

CUE1: A Mesophyll Cell-Specific Positive Regulator of Light-Controlled Gene Expression in Arabidopsis.

et al. 1995

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Light plays a key role in the development and physiology of plants. One of the most profound effects of light on plant development is the derepression of expression of an array of light-responsive genes, including the genes encoding the chlorophyll alb binding proteins (CAB) of photosystem II. To understand the mechanism by which light signals nuclear gene expression, we developed a genetic selection to identify mutants with reduced CAB transcription. Here, we describe a new Arabidopsis locus, COE7 (for CAS ynderexpressed). Mutations at this locus result in defects in expression of several light-regulated genes, specifically in mesophyll but not in bundle-associated or epidermis cells. Reduced accumulation of CAB and other photosynthesis-related mRNAs in the mesophyll was correlated with defects in chloroplast development in these cells, resulting in a reticulate pattern with veins greener than the interveinal regions of leaves.Moreover, chalcone synthase mRNA, although known to be regulated by both phytochrome and a blue light receptor, accumulated normally in the leaf epidermis. Dark basal levels of CAB expression were unaffected in etiolated cueí seedlings; however, induction of CAB transcription by pulses of red and blue light was reduced, suggesting that CUEl acts downstream from both phytochrome and blue light photoreceptors. CUEl appears to play a role in the primary derepression of mesophyll-specific gene expression in response to light, because cueí mutants are severely deficient at establishing photoautotrophic growth. Based on this characterization, we propose that CUEí is a cell-specific positive regulator linking light and intrinsic developmental programs in Arabidopsis leaf mesophyll cells.

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Section: Discussionmentioning

confidence: 99%

CUE1: A Mesophyll Cell-Specific Positive Regulator of Light-Controlled Gene Expression in Arabidopsis.

et al. 1995

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“…Second, promoters with paired LREs or a single LRE respond differently to the photomorphogenic repressor COP1 and DET1. The cop1 and det1 mutations lead to activation of many light-induced promoters in the dark (Chory et al, 1989;. Not surprisingly, cop1-4 and det1-1 mutations led to light-independent expression of the CAB1 promoter as well as light-inducible synthetic promoters (Figure 8).…”

Section: Light Regulation Of Synthetic Promoters Containing Paired Lrmentioning

confidence: 92%

“…The cop1-4 McNellis et al, 1994a) and det1-1 (Chory et al, 1989;Pepper et al, 1994) mutations used for this study have been described previously as indicated. All the promoter::GUS fusion constructs have been described by Puente et al (1996).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Combinatorial interaction of light‐responsive elements plays a critical role in determining the response characteristics of light‐regulated promoters in Arabidopsis

et al. 1998

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SummaryWe have studied the roles of PhyA, PhyB and CRY1 photoreceptors and the downstream light-signaling components, COP1 and DET1, in mediating high-irradiance light-controlled activity of promoters containing synthetic light-responsive elements (LRE). Promoters with paired LREs were able to respond to a wide spectrum of light through multiple photoreceptors, while the light-inducible single LRE promoters primarily responded to a specific wavelength of light. In addition, our results indicate that Cry1 is involved in PhyB-mediated red-light induction of the G-GATA/NOS101 promoter, and that both Cry1 and PhyB are required for effective repression of the GT1/ NOS101 promoter by red or blue light. An interaction between PhyA and PhyB in mediating GT1-GATA/NOS101 promoter light activation was also observed. Furthermore, our data indicate that COP1 and DET1 exert negative control in the dark only on paired LRE promoters but not single LRE promoters. From these results, we conclude that the combinatorial interaction of LREs is essential in determining the ability of light-responsive promoters to be modulated by crucial cellular regulators and to respond to diverse light environments.

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“…The Rim2 expression level was quanti®ed using a Phosphor Image System (Molecular Dynamics, Sunnyvale, Calif.), its expression level at time 0 was set at 1. The Northern blots were reprobed with the 18S Arabidopsis rDNA (Chory et al 1989) to normalize for variations in RNA loading.…”

Section: Northern and Southern Hybridizationsmentioning

confidence: 99%

The rice Rim2 transcript accumulates in response to Magnaporthe grisea and its predicted protein product shares similarity with TNP2-like proteins encoded by CACTA transposons

Dong

et al. 2000

Mol Gen Genet

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Arabidopsis thaliana mutant that develops as a light-grown plant in the absence of light

Cited by 458 publications

References 26 publications

CUE1: A Mesophyll Cell-Specific Positive Regulator of Light-Controlled Gene Expression in Arabidopsis.

CUE1: A Mesophyll Cell-Specific Positive Regulator of Light-Controlled Gene Expression in Arabidopsis.

Combinatorial interaction of light‐responsive elements plays a critical role in determining the response characteristics of light‐regulated promoters in Arabidopsis

The rice Rim2 transcript accumulates in response to Magnaporthe grisea and its predicted protein product shares similarity with TNP2-like proteins encoded by CACTA transposons

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